EIF3A Antibody | Affinity Biosciences

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IHC
IF/ICC

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EIF3A Antibody - Western blot analysis of extracts from Huvec, using EIF3A Antibody.

EIF3A Antibody - DF9510 at 1/100 staining Mouse spleen tissue by IHC-P.

EIF3A Antibody - DF9510 staining Hela cells by IF/ICC.

Product:	EIF3A Antibody
Catalog:	DF9510
Description:	Rabbit polyclonal antibody to EIF3A
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	167kD; 167kD(Calculated).
Uniprot:	Q14152
RRID:	AB_2842706

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

EIF3A Antibody detects endogenous levels of total EIF3A.

RRID:

AB_2842706
Cite Format: Affinity Biosciences Cat# DF9510, RRID:AB_2842706.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

centrosomin homolog; cytoplasmic protein p167; eIF 3 theta; eIF-3-theta; EIF3; eIF3 p167; eIF3 p170; eIF3 p180; EIF3 p180 subunit; eIF3 p185; eIF3 theta; eIF3a; EIF3A_HUMAN; EIF3S10; eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD); eukaryotic translation initiation factor 3 subunit 10 (theta 170kD); eukaryotic translation initiation factor 3 subunit 10 170kDa; Eukaryotic translation initiation factor 3 subunit 10; eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa; Eukaryotic translation initiation factor 3 subunit A; OTTHUMP00000020589; P167; p180; p185; TIF32;

Immunogens

Immunogen:

Uniprot:

Q14152(EIF3A_HUMAN) >>Visit HPA database.

Gene(ID):

EIF3A(8661)

Sequence:

Q14152 EIF3A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPAYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQMVLDIEDLDNIQTPESVLLSAVSGEDTQDRTDRLLLTPWVKFLWESYRQCLDLLRNNSRVERLYHDIAQQAFKFCLQYTRKAEFRKLCDNLRMHLSQIQRHHNQSTAINLNNPESQSMHLETRLVQLDSAISMELWQEAFKAVEDIHGLFSLSKKPPKPQLMANYYNKVSTVFWKSGNALFHASTLHRLYHLSREMRKNLTQDEMQRMSTRVLLATLSIPITPERTDIARLLDMDGIIVEKQRRLATLLGLQAPPTRIGLINDMVRFNVLQYVVPEVKDLYNWLEVEFNPLKLCERVTKVLNWVREQPEKEPELQQYVPQLQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDLQVRIDHTSRTLSFGSDLNYATREDAPIGPHLQSMPSEQIRNQLTAMSSVLAKALEVIKPAHILQEKEEQHQLAVTAYLKNSRKEHQRILARRQTIEERKERLESLNIQREKEELEQREAELQKVRKAEEERLRQEAKEREKERILQEHEQIKKKTVRERLEQIKKTELGAKAFKDIDIEDLEELDPDFIMAKQVEQLEKEKKELQERLKNQEKKIDYFERAKRLEEIPLIKSAYEEQRIKDMDLWEQQEEERITTMQLEREKALEHKNRMSRMLEDRDLFVMRLKAARQSVYEEKLKQFEERLAEERHNRLEERKRQRKEERRITYYREKEEEEQRRAEEQMLKEREERERAERAKREEELREYQERVKKLEEVERKKRQRELEIEERERRREEERRLGDSSLSRKDSRWGDRDSEGTWRKGPEADSEWRRGPPEKEWRRGEGRDEDRSHRRDEERPRRLGDDEDREPSLRPDDDRVPRRGMDDDRGPRRGPEEDRFSRRGADDDRPSWRNTDDDRPPRRIADEDRGNWRHADDDRPPRRGLDEDRGSWRTADEDRGPRRGMDDDRGPRRGGADDERSSWRNADDDRGPRRGLDDDRGPRRGMDDDRGPRRGMDDDRGPRRGMDDDRGPRRGLDDDRGPWRNADDDRIPRRGAEDDRGPWRNMDDDRLSRRADDDRFPRRGDDSRPGPWRPLVKPGGWREKEKAREESWGPPRESRPSEEREWDREKERDRDNQDREENDKDPERERDRERDVDREDRFRRPRDEGGWRRGPAEESSSWRDSSRRDDRDRDDRRRERDDRRDLRERRDLRDDRDRRGPPLRSEREEVSSWRRADDRKDDRVEERDPPRRVPPPALSRDRERDRDREREGEKEKASWRAEKDRESLRRTKNETDEDGWTTVRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14152 As Substrate

Q14152

Site	PTM Type	Source
Y4	Phosphorylation	Uniprot
K13	Methylation	Uniprot
K13	Ubiquitination	Uniprot
K23	Ubiquitination	Uniprot
K24	Ubiquitination	Uniprot
Y32	Phosphorylation	Uniprot
K45	Ubiquitination	Uniprot
K68	Acetylation	Uniprot
K68	Ubiquitination	Uniprot
Y72	Phosphorylation	Uniprot
K75	Ubiquitination	Uniprot
K84	Ubiquitination	Uniprot
K95	Ubiquitination	Uniprot
K105	Ubiquitination	Uniprot
T147	Phosphorylation	Uniprot
K196	Acetylation	Uniprot
K196	Ubiquitination	Uniprot
S261	Phosphorylation	Uniprot
K265	Ubiquitination	Uniprot
K268	Ubiquitination	Uniprot
K285	Ubiquitination	Uniprot
Y300	Phosphorylation	Uniprot
S303	Phosphorylation	Uniprot
K308	Ubiquitination	Uniprot
T311	Phosphorylation	Uniprot
T326	Phosphorylation	Uniprot
S328	Phosphorylation	Uniprot
T332	Phosphorylation	Uniprot
K351	Ubiquitination	Uniprot
Y382	Phosphorylation	Uniprot
K402	Ubiquitination	Uniprot
K409	Ubiquitination	Uniprot
K420	Ubiquitination	Uniprot
C478	S-Nitrosylation	Uniprot
S492	Phosphorylation	Uniprot
Y499	Phosphorylation	Uniprot
S528	Phosphorylation	Uniprot
K538	Ubiquitination	Uniprot
K559	Methylation	Uniprot
T574	Phosphorylation	Uniprot
S584	Phosphorylation	Uniprot
K591	Ubiquitination	Uniprot
K603	Ubiquitination	Uniprot
K617	Ubiquitination	Uniprot
K621	Ubiquitination	Uniprot
K632	Methylation	Uniprot
K632	Ubiquitination	Uniprot
K633	Methylation	Uniprot
K633	Ubiquitination	Uniprot
K634	Methylation	Uniprot
T635	Phosphorylation	Uniprot
T646	Phosphorylation	Uniprot
K651	Ubiquitination	Uniprot
K654	Ubiquitination	Uniprot
K672	Ubiquitination	Uniprot
K679	Ubiquitination	Uniprot
K682	Ubiquitination	Uniprot
K689	Ubiquitination	Uniprot
K693	Acetylation	Uniprot
K694	Acetylation	Uniprot
K694	Ubiquitination	Uniprot
R700	Methylation	Uniprot
K711	Ubiquitination	Uniprot
K720	Ubiquitination	Uniprot
R757	Methylation	Uniprot
K775	Ubiquitination	Uniprot
K777	Ubiquitination	Uniprot
R782	Methylation	Uniprot
K824	Ubiquitination	Uniprot
R842	Methylation	Uniprot
Y844	Phosphorylation	Uniprot
S881	Phosphorylation	Uniprot
S882	Phosphorylation	Uniprot
S884	Phosphorylation	Uniprot
S888	Phosphorylation	Uniprot
S895	Phosphorylation	Uniprot
T898	Phosphorylation	Uniprot
S949	Phosphorylation	Uniprot
R956	Methylation	Uniprot
R960	Methylation	Uniprot
R966	Methylation	Uniprot
R976	Methylation	Uniprot
S978	Phosphorylation	Uniprot
R986	Methylation	Uniprot
S988	Phosphorylation	Uniprot
R1006	Methylation	Uniprot
R1026	Methylation	Uniprot
S1028	Phosphorylation	Uniprot
R1040	Methylation	Uniprot
R1046	Methylation	Uniprot
R1057	Methylation	Uniprot
R1071	Methylation	Uniprot
R1081	Methylation	Uniprot
R1087	Methylation	Uniprot
R1091	Methylation	Uniprot
R1097	Methylation	Uniprot
R1101	Methylation	Uniprot
R1107	Methylation	Uniprot
R1111	Methylation	Uniprot
R1117	Methylation	Uniprot
R1127	Methylation	Uniprot
R1137	Methylation	Uniprot
S1149	Phosphorylation	Uniprot
R1156	Methylation	Uniprot
K1174	Acetylation	Uniprot
S1188	Phosphorylation	Uniprot
S1198	Phosphorylation	Uniprot
R1238	Methylation	Uniprot
S1256	Phosphorylation	Uniprot
S1257	Phosphorylation	Uniprot
S1262	Phosphorylation	Uniprot
S1309	Phosphorylation	Uniprot
S1336	Phosphorylation	Uniprot
K1351	Acetylation	Uniprot
S1355	Phosphorylation	Uniprot
S1364	Phosphorylation	Uniprot
T1368	Phosphorylation	Uniprot
T1372	Phosphorylation	Uniprot
T1378	Phosphorylation	Uniprot
T1379	Phosphorylation	Uniprot

Research Backgrounds

Q14152_EIF3A_HUMAN

Function:

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

(Microbial infection) Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation.

(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

PTMs:

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Interacts with EIF4G1 (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2.

Family&Domains:

Belongs to the eIF-3 subunit A family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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EIF3A Antibody - #DF9510