Product: Cytokeratin 10 Antibody
Catalog: AF0197
Description: Rabbit polyclonal antibody to Cytokeratin 10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 59kDa; 59kD(Calculated).
Uniprot: P13645
RRID: AB_2833390

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Bovine(95%), Sheep(95%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 10 Antibody detects endogenous levels of total Cytokeratin 10.
RRID:
AB_2833390
Cite Format: Affinity Biosciences Cat# AF0197, RRID:AB_2833390.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BCIE; BIE; CK 10; CK-10; Cytokeratin-10; EHK; K10; K1C10_HUMAN; Keratin 10; Keratin 10 type I; Keratin; Keratin type i cytoskeletal 10; Keratin type I cytoskeletal 59 kDa; Keratin-10; Keratin10; KPP; KRT10; type I cytoskeletal 10;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P13645 K1C10_HUMAN:

Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines (PubMed:19627498).

Description:
K10 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Sequence:
MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGIFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Bovine
95
Sheep
95
Pig
89
Chicken
58
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13645 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
Y11 Phosphorylation
S13 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S38 Phosphorylation
S39 Phosphorylation
S42 Phosphorylation
S53 Phosphorylation
S56 Phosphorylation
S62 Phosphorylation
S71 Phosphorylation
S159 Phosphorylation
Y160 Phosphorylation
K163 Acetylation
K163 Ubiquitination
S170 Phosphorylation
Y172 Phosphorylation
S188 Phosphorylation
Y199 Phosphorylation
Y238 Phosphorylation
T277 Phosphorylation
Y282 Phosphorylation
K284 Sumoylation
K284 Ubiquitination
S298 Phosphorylation
Y325 Phosphorylation
K343 Ubiquitination
S344 Phosphorylation
T430 Phosphorylation
S458 Phosphorylation
S459 Phosphorylation
S535 Phosphorylation
S546 Phosphorylation
Y557 Phosphorylation
S568 Phosphorylation
S569 Phosphorylation
S571 Phosphorylation
S573 Phosphorylation
S577 Phosphorylation
S578 Phosphorylation
S579 Phosphorylation
Y584 Phosphorylation

Research Backgrounds

Function:

Plays a role in the establishment of the epidermal barrier on plantar skin.

(Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.

(Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.

Subcellular Location:

Secreted>Extracellular space. Cell surface.
Note: Localized on the surface of desquamated nasal epithelial cells (PubMed:12427098). Localized on the surface of lung cell lines (PubMed:19627498).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Heterodimer with KRT1. Two heterodimers of KRT1 and KRT10 form a heterotetramer. The KRT10 subunit in the heterotetramer is probably disulfide-linked (Probable).

(Microbial infection) Interacts (via C-terminal tail domain) with the S.aureus clumping factor, clfB; this interaction probably mediates S.aureus attachment to the keratinized squamous epithelial cells from the nasal cavity.

(Microbial infection) Interacts (via the C-terminal tail domain) with S.pneumoniae serine-rich repeat protein PsrP; this interaction probably mediates S.pneumoniae adherence to lung tissue and subsequent pathogenesis. Neither protein has to be glycosylated for the interaction to occur.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). Chen R et al. Alantolactone-loaded chitosan/hyaluronic acid nanoparticles suppress psoriasis by deactivating STAT3 pathway and restricting immune cell recruitment. Asian Journal of Pharmaceutical Sciences 2022 Mar;17(2):268-283. (PubMed: 35582636) [IF=10.2]

Application: IHC    Species: Mice    Sample: skin tissue

Fig. 6 CHALT suppresses keratinocyte proliferation in IMQ-induced skin. The skin was evaluated by (A) Masson's trichrome staining, and quantitative analysis was shown (D). Immunohistochemical staining of (B) CK10 and (C) Ki 67 was performed, and quantitative analysis was shown in (E) and (F), respectively. Scale bar: 100 µM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, indicate statistical difference compared to model group or between groups. ns indicates no statistical significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Application: IHC    Species: mouse    Sample: skin

Fig. 6|CHALT suppresses keratinocyte proliferation in IMQ-induced skin. The skin was evaluated by (A) Masson’s trichrome staining, and quantitative analysis was shown (D). Immunohistochemical staining of (B) CK10 and (C) Ki 67 was performed,and quantitative analysis was shown in (E) and (F), respectively.

2). Wang S et al. Stem cell membrane-coated isotretinoin for acne treatment. JOURNAL OF NANOBIOTECHNOLOGY 2020 Jul 28;18(1):106 (PubMed: 32723398) [IF=10.2]

3). Yao Q et al. Water-responsive gel extends drug retention and facilitates skin penetration for curcumin topical delivery against psoriasis. Asian Journal of Pharmaceutical Sciences 2023 Mar;18(100782) (PubMed: 36845839) [IF=10.2]

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