DFNA5/GSDME Antibody - #DF9705

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Product: DFNA5/GSDME Antibody
Catalog: DF9705
Description: Rabbit polyclonal antibody to DFNA5/GSDME
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Mol.Wt.: 55 kDa; 55kD(Calculated).
Uniprot: O60443
RRID: AB_2842900

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
DFNA5/GSDME Antibody detects endogenous levels of total DFNA5/GSDME.
RRID:
AB_2842900
Cite Format: Affinity Biosciences Cat# DF9705, RRID:AB_2842900.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2310037D07Rik; 4932441K13Rik; Deafness, autosomal dominant 5; Deafness, autosomal dominant 5 protein; DFNA5; DFNA5 gene; DFNA5_HUMAN; Dfna5h; EG14210; Fin15; ICERE 1; ICERE-1; Inversely correlated with estrogen receptor expression 1; Non-syndromic hearing impairment protein 5; Nonsyndromic hearing impairment protein; Gasdermin-E;GSDME_HUMAN;ICERE1;

Immunogens

Immunogen:

A synthesized peptide derived from human DFNA5/GSDME, corresponding to a region within C-terminal amino acids.

Uniprot:

O60443(GSDME_HUMAN) >>Visit HPA database.

Gene(ID):
DFNA5(1687)
Expression:
O60443 GSDME_HUMAN:

Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.

Sequence:
MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS

Research Backgrounds

Function:

Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53.

Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis. Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents. Exhibits bactericidal activity.

PTMs:

Cleavage at Asp-270 by CASP3 (mature and uncleaved precursor forms) relieves autoinhibition and is sufficient to initiate pyroptosis.

Subcellular Location:

Cell membrane.

Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in cochlea. Low level of expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas, with highest expression in placenta.

Family&Domains:

Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of activation signal. The intrinsic pyroptosis-inducing activity is carried by the N-terminal domain, that is released upon cleavage by CASP3.

Belongs to the gasdermin family.

References

1). Platelets camouflaged nanovehicle improved bladder cancer immunotherapy by triggering pyroptosis. Theranostics, 2024 (PubMed: 39479459) [IF=12.4]

Application: WB    Species: Mouse    Sample: MBT-2 cells

Figure 1. The concentration-dependent induction of pyroptosis by nano-Erda. (A) Protein expression of key pyroptotic molecules, including caspase-3, cleaved caspase-3, GSDME (full-length GSDME), and GSDME-N (N-terminal-cleaved GSDME). The MBT-2 cells were treated with concentrations of 0, 10, 20, and 40 μM of nano-Erda for 24 hours. (B) Immunofluorescence detection of cleaved caspase-3 after the MBT-2 cells were treated with concentrations of nano-Erda. (C) Immunofluorescence detection of GSDME-N. (D) The concentrations of LDH released from MBT-2 cells treated with different concentrations of nano-Erda for 24 h. (E) The concentrations of HMGB1 released from MBT-2 cells. (F) The concentrations of IL-1β released from MBT-2 cells. (G) The concentrations of IL-18 released from MBT-2 cells. (H) Representative flow cytometry results of nano-Erda treated MBT-2 cells, which are Annexin V-PI- (LL), Annexin V+PI- (LR), Annexin V-PI+ (UL), or Annexin V+PI+ (UR). (I) viability (% of Annexin V+PI+ cells). *Significantly different compared to control (P < 0.05). **P < 0.01, ***P < 0.001, ****P < 0.0001.
2). Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination. Cell communication and signaling : CCS, 2023 (PubMed: 38102610) [IF=8.4]
3). Membrane Attack Complex C5b-9 Promotes Renal Tubular Epithelial Cell Pyroptosis in Trichloroethylene-Sensitized Mice. Frontiers in Pharmacology, 2022 (PubMed: 35656289) [IF=5.6]
4). Caspase-3 and gasdermin E mediate macrophage pyroptosis in periodontitis. Journal of periodontal research, 2024 (PubMed: 37885312) [IF=3.4]

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