Product: PRRX2 Antibody
Catalog: DF9729
Description: Rabbit polyclonal antibody to PRRX2
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 27 kDa; 27kD(Calculated).
Uniprot: Q99811
RRID: AB_2842924

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
PRRX2 Antibody detects endogenous levels of total PRRX2.
RRID:
AB_2842924
Cite Format: Affinity Biosciences Cat# DF9729, RRID:AB_2842924.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Paired mesoderm homeobox protein 2; Paired related homeobox protein 2; paired-like homeobox gene 2; paired-like homeodomain protein PRX2; Paired-related homeobox protein 2; PMX2; Predicted NUP98/PRRX2 fusion protein; Prrx2; PRRX2_HUMAN; PRX 2; PRX-2; PRX2;

Immunogens

Immunogen:

A synthesized peptide derived from human PRRX2, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q99811 PRRX2_HUMAN:

In fetal skin, highest expression found in cells of mesodermal origin within the dermal papilla of the developing hair shaft. Not detected in epidermis or dermis. In adult skin, weakly expressed within the basal layers of the epidermis. Not expressed in dermis.

Sequence:
MDSAAAAFALDKPALGPGPPPPPPALGPGDCAQARKNFSVSHLLDLEEVAAAGRLAARPGARAEAREGAAREPSGGSSGSEAAPQDGECPSPGRGSAAKRKKKQRRNRTTFNSSQLQALERVFERTHYPDAFVREELARRVNLSEARVQVWFQNRRAKFRRNERAMLASRSASLLKSYSQEAAIEQPVAPRPTALSPDYLSWTASSPYSTVPPYSPGSSGPATPGVNMANSIASLRLKAKEFSLHHSQVPTVN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
88
Bovine
88
Sheep
88
Dog
88
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May play a role in the scarless healing of cutaneous wounds during the first two trimesters of development.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In fetal skin, highest expression found in cells of mesodermal origin within the dermal papilla of the developing hair shaft. Not detected in epidermis or dermis. In adult skin, weakly expressed within the basal layers of the epidermis. Not expressed in dermis.

Family&Domains:

Belongs to the paired homeobox family.

References

1). Spinal-Specific Super Enhancer in Neuropathic Pain. The Journal of neuroscience : the official journal of the Society for Neuroscience, 2023 (PubMed: 37802656) [IF=4.4]

Application: WB    Species: Mouse    Sample:

Figure 2. Suppression of Ntmt1/Prrx2-SE activity by a pharmacological BRD4 inhibitor alleviates neuropathic pain sensitivities. a, The expression of BRD4 in dorsal horn on day 7 after CCI surgery was not changed; n ¼ 6 mice/group. b, Binding activity of BRD4 to three SS-SE clusters. ChIP-PCR with anti-BRD4 in the dorsal horn of Sham mice or CCI mice on day 7 after surgery; n ¼ 4 mice/group’ *p , 0.05, **p , 0.01 versus Sham. c, Binding activity of RNA polymerase II (RPII) to Ntmt1/Prrx2-SE clusters. ChIP-PCR with anti-RPII in the dorsal horn of Sham mice or CCI mice on day 7 after surgery; n ¼ 3 mice/group; ***p , 0.001 versus Sham1DMSO; # p , 0.05, ##p , 0.01 versus CCI1DMSO. d, Intrathecal injection of JQ1 7 d after CCI reduced the activity of Ntmt1/Prrx2-SE in ipsilateral dorsal horn 24 h after injection. SE activity was identified by H3K27ac-ChIP-qPCR. DMSO was used as the vehicle control; n ¼ 4 mice/group, *p , 0.05, **p , 0.01, ***p , 0.001 versus Sham1DMSO; # p , 0.05, ###p , 0.001, CCI1DMSO. e–j, Time course of the effect of intrathecal injection of JQ1 or vehicle control into CCI or sham male (e–g) and female (h–j) mice on the ipsilateral PWF to 0.07 g and 0.4 g von Frey filaments and on PWLs to heat stimuli; n ¼ 8 mice/group, ***p , 0.001, versus the Sham1DMSO group at the corresponding time points; # p , 0.05, ##p , 0.01, ###p , 0.001 versus CCI1DMSO at the corresponding time points; two-way ANOVA, post hoc Tukey’s tests. Red arrows indicate CCI or sham surgery. Blue arrows indicate JQ1 or vehicle control injection.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.