PALLD Antibody | Affinity Biosciences

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IF/ICC
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PALLD Antibody - Western blot analysis of extracts from various samples, using PALLD Antibody.

PALLD Antibody - DF9731 staining Hela cells by IF/ICC.

PALLD Antibody - Figure 3:| OLA disrupts actin microfilaments organization in Sertoli cells.

Product:	PALLD Antibody
Catalog:	DF9731
Description:	Rabbit polyclonal antibody to PALLD
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	151kDa; 151kD(Calculated).
Uniprot:	Q8WX93
RRID:	AB_2842926

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(88%), Horse(100%), Sheep(80%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

PALLD Antibody detects endogenous levels of total PALLD.

RRID:

AB_2842926
Cite Format: Affinity Biosciences Cat# DF9731, RRID:AB_2842926.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CGI 151; CGI151; KIAA0992; MYN; Myoneurin; pa; Palladin cytoskeletal associated protein; Palld; PNCA1; Sarcoma antigen NY SAR 77; SIH002; Stx13bp1;

Immunogens

Immunogen:

Uniprot:

Q8WX93(PALLD_HUMAN) >>Visit HPA database.

Gene(ID):

PALLD(23022)

Expression:

Q8WX93 PALLD_HUMAN:

Detected in both muscle and non-muscle tissues. High expression in prostate, ovary, colon, and kidney. Not detected in spleen, skeletal muscle, lung and peripheral blood lymphocytes (at protein level). Protein is overexpressed in FA6, HPAF, IMIM-PC2, SUIT-2 and PancTu-II sporadic pancreatic cancer cell lines.

Sequence:

Q8WX93 PALLD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGTSSHESFYDSLSDMQEESKNTDFFPGLSAFLSQEEINKSLDLARRAIADSETEDFDSEKEISQIFSTSPASLCEHPSHKETKLGEHASRRPQDNRSTPVQPLAEKQTKSISSPVSKRKPAMSPLLTRPSYIRSLRKAEKRGAKTPSTNVKPKTPHQRKGGPQSQLCDKAANLIEELTSIFKAAKPRNRSPNGESSSPDSGYLSPKNQPSALLSASASQSPMEDQGEMEREVKSPGARHCYQDNQDLAVPHNRKSHPQPHSALHFPAAPRFIQKLRSQEVAEGSRVYLECRVTGNPTPRVRWFCEGKELHNTPDIQIHCEGGDLHTLIIAEAFEDDTGRYTCLATNPSGSDTTSAEVFIEGASSTDSDSESLAFKSRAGAMPQAQKKTTSVSLTIGSSSPKTGVTTAVIQPLSVPVQQVHSPTSYLCRPDGTTTAYFPPVFTKELQNTAVAEGQVVVLECRVRGAPPLQVQWFRQGSEIQDSPDFRILQKKPRSTAEPEEICTLVIAETFPEDAGIFTCSARNDYGSATSTAQLVVTSANTENCSYESMGESNNDHFQHFPPPPPILETSSLELASKKPSEIQQVNNPELGLSRAALQMQFNAAERETNGVHPSRGVNGLINGKANSNKSLPTPAVLLSPTKEPPPLLAKPKLDPLKLQQLQNQIRLEQEAGARQPPPAPRSAPPSPPFPPPPAFPELAACTPPASPEPMSALASRSAPAMQSSGSFNYARPKQFIAAQNLGPASGHGTPASSPSSSSLPSPMSPTPRQFGRAPVPPFAQPFGAEPEAPWGSSSPSPPPPPPPVFSPTAAFPVPDVFPLPPPPPPLPSPGQASHCSSPATRFGHSQTPAAFLSALLPSQPPPAAVNALGLPKGVTPAGFPKKASRTARIASDEEIQGTKDAVIQDLERKLRFKEDLLNNGQPRLTYEERMARRLLGADSATVFNIQEPEEETANQEYKVSSCEQRLISEIEYRLERSPVDESGDEVQYGDVPVENGMAPFFEMKLKHYKIFEGMPVTFTCRVAGNPKPKIYWFKDGKQISPKSDHYTIQRDLDGTCSLHTTASTLDDDGNYTIMAANPQGRISCTGRLMVQAVNQRGRSPRSPSGHPHVRRPRSRSRDSGDENEPIQERFFRPHFLQAPGDLTVQEGKLCRMDCKVSGLPTPDLSWQLDGKPVRPDSAHKMLVRENGVHSLIIEPVTSRDAGIYTCIATNRAGQNSFSLELVVAAKEAHKPPVFIEKLQNTGVADGYPVRLECRVLGVPPPQIFWKKENESLTHSTDRVSMHQDNHGYICLLIQGATKEDAGWYTVSAKNEAGIVSCTARLDVYTQWHQQSQSTKPKKVRPSASRYAALSDQGLDIKAAFQPEANPSHLTLNTALVESEDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Zebrafish

Sheep

Pig

Bovine

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WX93 As Substrate

Q8WX93

Site	PTM Type	Enzyme	Source
S53	Phosphorylation		Uniprot
S99	Phosphorylation		Uniprot
T110	Phosphorylation		Uniprot
S112	Phosphorylation		Uniprot
S114	Phosphorylation		Uniprot
S115	Phosphorylation		Uniprot
S118	Phosphorylation		Uniprot
S125	Phosphorylation		Uniprot
Y133	Phosphorylation		Uniprot
S136	Phosphorylation		Uniprot
S192	Phosphorylation		Uniprot
S356	Phosphorylation		Uniprot
S392	Phosphorylation		Uniprot
S394	Phosphorylation		Uniprot
S401	Phosphorylation		Uniprot
S423	Phosphorylation		Uniprot
S479	Phosphorylation		Uniprot
S484	Phosphorylation		Uniprot
S595	Phosphorylation		Uniprot
S632	Phosphorylation		Uniprot
T635	Phosphorylation		Uniprot
S641	Phosphorylation	P06493 (CDK1)	Uniprot
K659	Ubiquitination		Uniprot
S684	Phosphorylation		Uniprot
S688	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
T704	Phosphorylation		Uniprot
S708	Phosphorylation		Uniprot
S713	Phosphorylation		Uniprot
S717	Phosphorylation		Uniprot
S719	Phosphorylation		Uniprot
S725	Phosphorylation		Uniprot
S726	Phosphorylation		Uniprot
S728	Phosphorylation		Uniprot
Y731	Phosphorylation		Uniprot
S747	Phosphorylation		Uniprot
T751	Phosphorylation		Uniprot
S754	Phosphorylation		Uniprot
S755	Phosphorylation		Uniprot
S760	Phosphorylation		Uniprot
S763	Phosphorylation		Uniprot
S766	Phosphorylation	P06493 (CDK1)	Uniprot
R770	Methylation		Uniprot
S808	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
T877	Phosphorylation		Uniprot
S893	Phosphorylation		Uniprot
T900	Phosphorylation		Uniprot
K901	Ubiquitination		Uniprot
K915	Ubiquitination		Uniprot
C964	S-Nitrosylation		Uniprot
S970	Phosphorylation		Uniprot
Y974	Phosphorylation		Uniprot
S979	Phosphorylation		Uniprot
S984	Phosphorylation		Uniprot
Y990	Phosphorylation		Uniprot
K1029	Acetylation		Uniprot
K1029	Sumoylation		Uniprot
K1031	Acetylation		Uniprot
K1039	Acetylation		Uniprot
K1044	Ubiquitination		Uniprot
R1098	Methylation		Uniprot
S1101	Phosphorylation		Uniprot
S1104	Phosphorylation		Uniprot
S1106	Phosphorylation		Uniprot
S1116	Phosphorylation		Uniprot
S1118	Phosphorylation	P31749 (AKT1) , P31751 (AKT2)	Uniprot
S1121	Phosphorylation		Uniprot
T1163	Phosphorylation		Uniprot
S1179	Phosphorylation		Uniprot
K1232	Ubiquitination		Uniprot
Y1249	Phosphorylation		Uniprot
Y1326	Phosphorylation		Uniprot
T1327	Phosphorylation		Uniprot
S1333	Phosphorylation		Uniprot
S1335	Phosphorylation		Uniprot
K1337	Acetylation		Uniprot
Y1348	Phosphorylation		Uniprot
S1352	Phosphorylation		Uniprot
S1369	Phosphorylation		Uniprot
S1380	Phosphorylation		Uniprot

Research Backgrounds

Q8WX93_PALLD_HUMAN

Function:

Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci.

PTMs:

Phosphorylated predominantly on serines and, to a lesser extent, on tyrosines (By similarity). Phosphorylation at Ser-1118 by PKB/AKT1 modulates cytoskeletal organization and cell motility.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Focal adhesion. Cytoplasm>Myofibril>Sarcomere>Z line. Cell projection>Ruffle. Cell projection>Podosome. Cell projection>Lamellipodium. Cell projection>Axon. Cell projection>Growth cone.
Note: Localizes to stress fibers and Z lines (PubMed:11598191, PubMed:16125169, PubMed:17322171, PubMed:17537434). Preferentially expressed in the excitatory presynaptic terminals (By similarity).

Tissue Specificity:

Subunit Structure:

Interacts with EPS8 (By similarity). Interacts with LASP1 (By similarity). Interacts with VASP (By similarity). Interacts with ACTN. Interacts with ARGBP2. Interacts with PFN1. Interacts with LPP. Interacts with SPIN90. Interacts with SRC. Interacts with EZR. Interacts with RAI14 (By similarity).

Family&Domains:

Belongs to the myotilin/palladin family.

References

1). Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells. Oncotarget, 2017 (PubMed: 29179463)

Application: WB Species: mouse Sample: Sertoli cells

Figure 3:| OLA disrupts actin microfilaments organization in Sertoli cells.(B) Immunoblot analysis to assess the effects of OLA on the expression of actin regulatory proteins: Rac1, CDC42, N-WASP, Arp3 and Palladin. GAPDH served as protein loading control. Semiquantitative analysis of protein expression in following histogram (mean ± S.E.M., three independent replicates per groups). * p < 0.05; ** p < 0.01

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PALLD Antibody - #DF9731