RBM16 Antibody | Affinity Biosciences

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RBM16 Antibody - Western blot analysis of extracts from mouse muscle, using RBM16 Antibody.

Product:	RBM16 Antibody
Catalog:	DF9790
Description:	Rabbit polyclonal antibody to RBM16
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	141kDa; 141kD(Calculated).
Uniprot:	Q9UPN6
RRID:	AB_2842985

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

RBM16 Antibody detects endogenous levels of total RBM16.

RRID:

AB_2842985
Cite Format: Affinity Biosciences Cat# DF9790, RRID:AB_2842985.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CDC5L complex associated protein 7; CDC5L complex-associated protein 7; Protein SCAF8; putative RNA binding protein 16; RBM 16; RNA binding motif protein 16; RNA binding protein 16; RNA-binding motif protein 16; SCAF 8; SCAF8; SCAF8_HUMAN; SR like CTD associated factor 8; SR related and CTD associated factor 8; SR-related and CTD-associated factor 8;

Immunogens

Immunogen:

Uniprot:

Q9UPN6(SCAF8_HUMAN) >>Visit HPA database.

Gene(ID):

SCAF8(22828)

Sequence:

Q9UPN6 SCAF8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEAVKTFNSELYSLNDYKPPISKAKMTQITKAAIKAIKFYKHVVQSVEKFIQKCKPEYKVPGLYVIDSIVRQSRHQFGQEKDVFAPRFSNNIISTFQNLYRCPGDDKSKIVRVLNLWQKNNVFKSEIIQPLLDMAAGIPPPVVTPVLASTTTAMSNTPGTPVTPVTPANVVQGLPDPWVSQITNTDTLAAVAQILQSPQGQQLQQLIQTLQIQQQKPQPSILQALDAGLVVQLQALTAQLTAAAAAANTLTPLEQGVSFNKKLMDRFDFGEDSEHSEEPKKEIPASQLSHVSESVNNSIFHQIAEQLQQQNLEHLRQQLLEQQQPQKATPQDSQEGTFGSEHSASPSQGSSQQHFLEPEVNLDDSIDIQQQDMDIDEGQDGVEEEVFEQEAKKVAVRSRSRTHSRSRSRSPRKRRSRSRSGSRKRKHRKRSRSRSRERKRKSSRSYSSERRAREREKERQKKGLPPIRSKTLSVCSTTLWVGQVDKKATQQDLTNLFEEFGQIESINMIPPRGCAYVCMVHRQDAFRALQKLSSGSYKIGSKVIKIAWALNKGVKTEYKQFWDVDLGVTYIPWEKVKVDDLEGFAEGGMIDQETVNTEWETVKSSEPVKETVQTTQSPTPVEKETVVTTQAEVFPPPVAMLQIPVAPAVPTVSLVPPAFPVSMPVPPPGFSPIPPPPFLRASFNPSQPPPGFMPPPVPPPVVPPPTIPPVVPTSLVQPSLSMTPETVKDVGFGSLVIPGGSVASNLATSALPAGNVFNAPTKQAEPEEKVPHLIDHQISSGENTRSVIPNDISSNAAILGGQPPNVTSNSGILGVQRPNVSSNSEILGVRPSNVSSSSGIIAAQPPNILNNSGILGIQPPSVSNSSGLLGVLPPNIPNNSGLVGVQPPNVPNTPGLLGTQPPAGPQNLPPLSIPNQRMPTMPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UPN6 As Substrate

Q9UPN6

Site	PTM Type	Source
T6	Phosphorylation	Uniprot
Y12	Phosphorylation	Uniprot
Y17	Phosphorylation	Uniprot
K18	Sumoylation	Uniprot
K18	Ubiquitination	Uniprot
S22	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
K53	Ubiquitination	Uniprot
K81	Ubiquitination	Uniprot
K119	Ubiquitination	Uniprot
K124	Ubiquitination	Uniprot
S273	Phosphorylation	Uniprot
K281	Ubiquitination	Uniprot
S443	Phosphorylation	Uniprot
S445	Phosphorylation	Uniprot
S447	Phosphorylation	Uniprot
K461	Acetylation	Uniprot
S536	Phosphorylation	Uniprot
K542	Ubiquitination	Uniprot
K609	Acetylation	Uniprot
T611	Phosphorylation	Uniprot
T614	O-Glycosylation	Uniprot
T615	O-Glycosylation	Uniprot
T615	Phosphorylation	Uniprot
S617	Phosphorylation	Uniprot
T619	Phosphorylation	Uniprot
S779	Phosphorylation	Uniprot
S780	Phosphorylation	Uniprot
R917	Methylation	Uniprot
R927	Methylation	Uniprot
R938	Methylation	Uniprot
R967	Methylation	Uniprot
R981	Methylation	Uniprot
R988	Methylation	Uniprot
R1062	Methylation	Uniprot
R1073	Methylation	Uniprot
K1091	Ubiquitination	Uniprot
R1120	Methylation	Uniprot
R1122	Methylation	Uniprot
R1124	Methylation	Uniprot
R1129	Methylation	Uniprot
R1133	Methylation	Uniprot
R1139	Methylation	Uniprot
Y1198	Phosphorylation	Uniprot
Y1237	Phosphorylation	Uniprot

Research Backgrounds

Q9UPN6_SCAF8_HUMAN

Function:

Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation.

Subcellular Location:

Nucleus. Nucleus matrix.
Note: Detected in granular nuclear foci which correspond to sites of active transcription.

Subunit Structure:

Interacts with POLR2A; via C-terminal heptapeptide repeat domain (CTD) phosphorylated at 'Ser-2' and 'Ser-5'. Identified in a complex with CDC5L and other spliceosomal proteins.

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RBM16 Antibody - #DF9790