TRAPPC5 Antibody - #DF9965

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Product: TRAPPC5 Antibody
Catalog: DF9965
Description: Rabbit polyclonal antibody to TRAPPC5
Application: WB IHC
Cited expt.: IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 21 kDa; 21kD(Calculated).
Uniprot: Q8IUR0
RRID: AB_2843159

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TRAPPC5 Antibody detects endogenous levels of total TRAPPC5.
RRID:
AB_2843159
Cite Format: Affinity Biosciences Cat# DF9965, RRID:AB_2843159.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

A synthesized peptide derived from human TRAPPC5, corresponding to a region within the internal amino acids.

Uniprot:

Q8IUR0(TPPC5_HUMAN) >>Visit HPA database.

Gene(ID):
TRAPPC5(126003)
Sequence:
MEARFTRGKSALLERALARPRTEVSLSAFALLFSELVQHCQSRVFSVAELQSRLAALGRQVGARVLDALVAREKGARRETKVLGALLFVKGAVWKALFGKEADKLEQANDDARTFYIIEREPLINTYISVPKENSTLNCASFTAGIVEAVLTHSGFPAKVTAHWHKGTTLMIKFEEAVIARDRALEGR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May play a role in vesicular transport from endoplasmic reticulum to Golgi.

Subcellular Location:

Golgi apparatus>cis-Golgi network. Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TRAPP small subunits family. BET3 subfamily.

References

1). MCT4 Promotes Hepatocellular Carcinoma Progression by Upregulating TRAPPC5 Gene. Journal of hepatocellular carcinoma, 2022 (PubMed: 35425722) [IF=4.1]

Application: IHC    Species: human    Sample: HCC tissue

Figure 2 TRAPPC5 is overexpressed in HCC tissue microarrays and indicated poor prognosis. (A, B) TRAPPC5 is overexpressed in HCC tissue microarrays (n=21). TRAPPC5 protein was detected in cancer tissues in higher levels, whereas paracancerous tissues expressed much lower. Scale bars, 100 μm (magnification, 100×). (C) TRAPPC5 is highly expressed in HCC tissues (n=369) compared with that in normal tissues (n=160) using the GEPIA database. (D) Kaplan-Meier analysis of overall survival showed that patients with overexpression of TRAPPC5 have a poor prognosis (n=182) using the GEPIA database. *P < 0.05.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.