A-Myb Antibody - #AF9007

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Product: A-Myb Antibody
Catalog: AF9007
Description: Rabbit polyclonal antibody to A-Myb
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 85kDa; 86kD(Calculated).
Uniprot: P10243
RRID: AB_2843198

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
A-Myb Antibody detects endogenous levels of total A-Myb.
RRID:
AB_2843198
Cite Format: Affinity Biosciences Cat# AF9007, RRID:AB_2843198.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A-Myb; AMYB; MGC120059; MGC120061; Myb related protein A; Myb-like protein 1; Myb-related protein A; MYBA_HUMAN; MYBL1;

Immunogens

Immunogen:

A synthesized peptide derived from human A-Myb, corresponding to a region within the internal amino acids.

Uniprot:

P10243(MYBA_HUMAN) >>Visit HPA database.

Gene(ID):
MYBL1(4603)
Expression:
P10243 MYBA_HUMAN:

Expressed in a variety of lymphoid and solid tumor lines cultured in vitro.

Sequence:
MAKRSRSEDEDDDLQYADHDYEVPQQKGLKKLWNRVKWTRDEDDKLKKLVEQHGTDDWTLIASHLQNRSDFQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSLIAKHLKGRIGKQCRERWHNHLNPEVKKSSWTEEEDRIIYEAHKRLGNRWAEIAKLLPGRTDNSIKNHWNSTMRRKVEQEGYLQDGIKSERSSSKLQHKPCAAMDHMQTQNQFYIPVQIPGYQYVSPEGNCIEHVQPTSAFIQQPFIDEDPDKEKKIKELEMLLMSAENEVRRKRIPSQPGSFSSWSGSFLMDDNMSNTLNSLDEHTSEFYSMDENQPVSAQQNSPTKFLAVEANAVLSSLQTIPEFAETLELIESDPVAWSDVTSFDISDAAASPIKSTPVKLMRIQHNEGAMECQFNVSLVLEGKKNTCNGGNSEAVPLTSPNIAKFSTPPAILRKKRKMRVGHSPGSELRDGSLNDGGNMALKHTPLKTLPFSPSQFFNTCPGNEQLNIENPSFTSTPICGQKALITTPLHKETTPKDQKENVGFRTPTIRRSILGTTPRTPTPFKNALAAQEKKYGPLKIVSQPLAFLEEDIREVLKEETGTDLFLKEEDEPAYKSCKQENTASGKKVRKSLVLDNWEKEESGTQLLTEDISDMQSENRFTTSLLMIPLLEIHDNRCNLIPEKQDINSTNKTYTLTKKKPNPNTSKVVKLEKNLQSNCEWETVVYGKTEDQLIMTEQARRYLSTYTATSSTSRALIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Zebrafish
82
Xenopus
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Transcription factor that specifically recognizes the sequence 5'-YAAC[GT]G-3'. Acts as a master regulator of male meiosis by promoting expression of piRNAs: activates expression of both piRNA precursor RNAs and expression of protein-coding genes involved in piRNA metabolism (By similarity). The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons, which is essential for the germline integrity (By similarity). Transcriptional activator of SOX30 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a variety of lymphoid and solid tumor lines cultured in vitro.

References

1). MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma. Cell death & disease, 2022 (PubMed: 35987690) [IF=8.1]

Application: IHC    Species: human    Sample:

Fig. 1: Upregulation of MYBL1 correlates with high EV density and associates with HCC patient prognosis. A IHC staining indicating the MYBL1 and CD31 protein expression in para-tumor tissues and HCC tissues. B MYBL1 were positively correlated with CD31 protein levels in clinical specimens. C Patients with high MYBL1 expression (left), CD31 density (middle), and both MYBL1 and CD31 expression (right) show progression-free survival in HCC patients. D The correlation analysis between the mRNA levels of MYBL1 and angiogenesis in TCGA datasets.
2). Downregulation of MYBL1 in endothelial cells contributes to atherosclerosis by repressing PLEKHM1-inducing autophagy. Cell biology and toxicology, 2024 (PubMed: 38797732) [IF=5.3]

Application: WB    Species: human    Sample:

Fig. 2 Different expression of MYBL1, ARHGAP30 and FAM20A was observed in GSE28829, GSE43292 and GSE41571 datasets (A) The intersection of differentially expressed genes of GSE28829, GSE43292 and GSE41571 datasets was selected. (B) Heat map of MYBL1, ARHGAP30 and FAM20A genes in early and late atherosclerotic tissues. (C-E) GEPIA analysis of the three genes MYBL1, ARHGAP30, and FAM20A was performed to obtain box plots of their expression. (F) Immumohistochemical staining was used to determine the protein expression of MYBL1 in advanced atherosclerotic plaques (AA) and normal atherosclerotic plaques (NA) (n = 8). Scale bars = 200 μm or 20 μm. (G)The protein expression of MYBL1 was detected by western blot in early atherosclerosis and advanced atherosclerosis (n = 8). (H) The quantitative analysis of MYBL1 protein expression in early atherosclerosis and advanced atherosclerosis were shown. *p 

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