ARHGAP1 Antibody - #AF9011

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Product: ARHGAP1 Antibody
Catalog: AF9011
Description: Rabbit polyclonal antibody to ARHGAP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 50kDa; 50kD(Calculated).
Uniprot: Q07960
RRID: AB_2843202

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ARHGAP1 Antibody detects endogenous levels of total ARHGAP1.
RRID:
AB_2843202
Cite Format: Affinity Biosciences Cat# AF9011, RRID:AB_2843202.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGAP 1; Arhgap1; CDC42 GTPase activating protein; Cdc42 GTPase-activating protein; CDC42GAP; GTPase activating protein rhoOGAP; GTPase-activating protein rhoOGAP; GTPase-activating protein, Rho, 1; OTTHUMP00000233485; OTTHUMP00000233486; p50 RhoGAP; p50-RhoGAP; p50rhoGAP; RHG01_HUMAN; Rho GTPase activating protein 1; Rho GTPase-activating protein 1; Rho related small GTPase protein activator; Rho type GTPase activating protein 1; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; RHOGAP; RHOGAP1;

Immunogens

Immunogen:
Uniprot:

Q07960(RHG01_HUMAN) >>Visit HPA database.

Gene(ID):
ARHGAP1(392)
Expression:
Q07960 RHG01_HUMAN:

Ubiquitous.

Sequence:
MDPLSELQDDLTLDDTSEALNQLKLASIDEKNWPSDEMPDFPKSDDSKSSSPELVTHLKWDDPYYDIARHQIVEVAGDDKYGRKIIVFSACRMPPSHQLDHSKLLGYLKHTLDQYVESDYTLLYLHHGLTSDNKPSLSWLRDAYREFDRKYKKNIKALYIVHPTMFIKTLLILFKPLISFKFGQKIFYVNYLSELSEHVKLEQLGIPRQVLKYDDFLKSTQKSPATAPKPMPPRPPLPNQQFGVSLQHLQEKNPEQEPIPIVLRETVAYLQAHALTTEGIFRRSANTQVVREVQQKYNMGLPVDFDQYNELHLPAVILKTFLRELPEPLLTFDLYPHVVGFLNIDESQRVPATLQVLQTLPEENYQVLRFLTAFLVQISAHSDQNKMTNTNLAVVFGPNLLWAKDAAITLKAINPINTFTKFLLDHQGELFPSPDPSGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
92
Chicken
45
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07960 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
T12 Phosphorylation
T16 Phosphorylation
S17 Phosphorylation
S27 Phosphorylation
K31 Ubiquitination
S35 Phosphorylation
S44 Phosphorylation
S47 Phosphorylation
K48 Ubiquitination
S49 Phosphorylation
S50 Phosphorylation
S51 Phosphorylation
K59 Ubiquitination
Y64 Phosphorylation
Y65 Phosphorylation
K80 Acetylation
K80 Ubiquitination
Y81 Phosphorylation
Y144 Phosphorylation
Y159 Phosphorylation
T169 Phosphorylation
K212 Acetylation
K212 Ubiquitination
K218 Methylation
K218 Ubiquitination
T220 Phosphorylation
K222 Ubiquitination
S223 Phosphorylation
Y365 Phosphorylation
K411 Ubiquitination
S433 Phosphorylation
S437 Phosphorylation

Research Backgrounds

Function:

GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with BNIPL.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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