ARHGAP22 Antibody | Affinity Biosciences

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ARHGAP22 Antibody - Western blot analysis of ARHGAP22 using K562 whole cell lysates.

Product:	ARHGAP22 Antibody
Catalog:	AF9012
Description:	Rabbit polyclonal antibody to ARHGAP22
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	77kDa; 77kD(Calculated).
Uniprot:	Q7Z5H3
RRID:	AB_2843203

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

ARHGAP22 Antibody detects endogenous levels of total ARHGAP22.

RRID:

AB_2843203
Cite Format: Affinity Biosciences Cat# AF9012, RRID:AB_2843203.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARHGAP22; RHG22_HUMAN; Rho GTPase activating protein 2; Rho GTPase activating protein 22; Rho GTPase-activating protein 22; Rho type GTPase activating protein 22; Rho-type GTPase-activating protein 22; RhoGAP2; RhoGap22;

Immunogens

Immunogen:

Uniprot:

Q7Z5H3(RHG22_HUMAN) >>Visit HPA database.

Gene(ID):

ARHGAP22(58504)

Sequence:

Q7Z5H3 RHG22_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLSPKIRQARRARSKSLVMGEQSRSPGRMPCPHRLGPVLKAGWLKKQRSIMKNWQQRWFVLRGDQLFYYKDKDEIKPQGFISLQGTQVTELPPGPEDPGKHLFEISPGGAGEREKVPANPEALLLMASSQRDMEDWVQAIRRVIWAPLGGGIFGQRLEETVHHERKYGPRLAPLLVEQCVDFIRERGLTEEGLFRMPGQANLVRDLQDSFDCGEKPLFDSTTDVHTVASLLKLYLRELPEPVVPFARYEDFLSCAQLLTKDEGEGTLELAKQVSNLPQANYNLLRYICKFLDEVQAYSNVNKMSVQNLATVFGPNILRPQVEDPVTIMEGTSLVQHLMTVLIRKHSQLFTAPVPEGPTSPRGGLQCAVGWGSEEVTRDSQGEPGGPGLPAHRTSSLDGAAVAVLSRTAPTGPGSRCSPGKKVQTLPSWKSSFRQPRSLSGSPKGGGSSLEVPIISSGGNWLMNGLSSLRGHRRASSGDRLKDSGSVQRLSTYDNVPAPGLVPGIPSVASMAWSGASSSESSVGGSLSSCTACRASDSSARSSLHTDWALEPSPLPSSSEDPKSLDLDHSMDEAGAGASNSEPSEPDSPTREHARRSEALQGLVTELRAELCRQRTEYERSVKRIEEGSADLRKRMSRLEEELDQEKKKYIMLEIKLRNSERAREDAERRNQLLQREMEEFFSTLGSLTVGAKGARAPK

PTMs - Q7Z5H3 As Substrate

Q7Z5H3

Site	PTM Type	Enzyme	Source
S14	Phosphorylation		Uniprot
S16	Phosphorylation	P31749 (AKT1)	Uniprot
S209	Phosphorylation		Uniprot
T221	Phosphorylation		Uniprot
K260	Ubiquitination		Uniprot
S304	Phosphorylation		Uniprot
T310	Phosphorylation		Uniprot
S346	Phosphorylation		Uniprot
S359	Phosphorylation		Uniprot
S372	Phosphorylation		Uniprot
S394	Phosphorylation		Uniprot
S395	Phosphorylation		Uniprot
T424	Phosphorylation		Uniprot
S439	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot
S476	Phosphorylation		Uniprot
S587	Phosphorylation		Uniprot
S636	Phosphorylation		Uniprot
K648	Acetylation		Uniprot
K648	Methylation		Uniprot
Y649	Phosphorylation		Uniprot
K655	Acetylation		Uniprot

Research Backgrounds

Q7Z5H3_RHG22_HUMAN

Function:

Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. Acts as a GTPase activator for the RAC1 by converting it to an inactive GDP-bound state. Inhibits RAC1-dependent lamellipodia formation. May also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter (By similarity).

Subcellular Location:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic. Some fraction is nuclear (By similarity).

Subunit Structure:

Interacts with VEZF1.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ARHGAP22 Antibody - #AF9012