Arrdc1 Antibody - #AF9016
| Product: | Arrdc1 Antibody |
| Catalog: | AF9016 |
| Description: | Rabbit polyclonal antibody to Arrdc1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Rat |
| Prediction: | Pig, Bovine, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 48kDa; 46kD(Calculated). |
| Uniprot: | Q8N5I2 |
| RRID: | AB_2843207 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9016, RRID:AB_2843207.
Fold/Unfold
ARRD1_HUMAN; ARRDC 1; ARRDC1; Arrestin domain containing 1; Arrestin domain containing protein 1; Arrestin domain-containing protein 1; MGC40555; OTTHUMP00000022707; OTTHUMP00000022709; RP11-48C7.5;
Immunogens
- Q8N5I2 ARRD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGRVQLFEISLSHGRVVYSPGEPLAGTVRVRLGAPLPFRAIRVTCIGSCGVSNKANDTAWVVEEGYFNSSLSLADKGSLPAGEHSFPFQFLLPATAPTSFEGPFGKIVHQVRAAIHTPRFSKDHKCSLVFYILSPLNLNSIPDIEQPNVASATKKFSYKLVKTGSVVLTASTDLRGYVVGQALQLHADVENQSGKDTSPVVASLLQKVSYKAKRWIHDVRTIAEVEGAGVKAWRRAQWHEQILVPALPQSALPGCSLIHIDYYLQVSLKAPEATVTLPVFIGNIAVNHAPVSPRPGLGLPPGAPPLVVPSAPPQEEAEAEAAAGGPHFLDPVFLSTKSHSQRQPLLATLSSVPGAPEPCPQDGSPASHPLHPPLCISTGATVPYFAEGSGGPVPTTSTLILPPEYSSWGYPYEAPPSYEQSCGGVEPSLTPES
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8N5I2 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T58 | Phosphorylation | Uniprot | |
| S69 | Phosphorylation | Uniprot | |
| T117 | Phosphorylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| K337 | Ubiquitination | Uniprot | |
| Y405 | Phosphorylation | Uniprot |
Research Backgrounds
Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates. Through an ubiquitination-dependent mechanism plays for instance a role in the incorporation of SLC11A2 into extracellular vesicles. More generally, plays a role in the extracellular transport of proteins between cells through the release in the extracellular space of microvesicles. By participating to the ITCH-mediated ubiquitination and subsequent degradation of NOTCH1, negatively regulates the NOTCH signaling pathway.
Ubiquitinated. Ubiquitination by WWP2; promotes localization to extracellular microvesicles. Ubiquitinated by WWP1.
Cell membrane.
Note: Also found in extracellular vesicles different from exosomes.
Interacts (via PPxY motifs) with ITCH (via WW domains); the interaction is direct and participates to the recruitment of the ubiquitin-protein ligase ITCH to the NOTCH1 receptor. Interacts with ARRB1 and ARRB2; the interaction is direct. Interacts with TSG101; may recruit TSG101 to the plasma membrane. Interacts (via PPxY motifs) with WWP2 (via WW domains); ubiquitinates ARRDC1. Interacts with SLC11A2; controls the incorporation of SLC11A2 into extracellular vesicles through an ubiquitination-dependent mechanism. Interacts with WWP1 (via WW domains). Interacts with NEDD4 (via WW domains). Interacts with ALIX.
The PPxY motifs mediate interaction with WW domain-containing ubiquitin-protein ligases.
Belongs to the arrestin family.
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