BRD3 Antibody - #AF9025

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Product: BRD3 Antibody
Catalog: AF9025
Description: Rabbit polyclonal antibody to BRD3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 90kDa; 80kD(Calculated).
Uniprot: Q15059
RRID: AB_2843216

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(82%), Rabbit(91%), Dog(91%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
BRD3 Antibody detects endogenous levels of total BRD3.
RRID:
AB_2843216
Cite Format: Affinity Biosciences Cat# AF9025, RRID:AB_2843216.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BRD 3; Brd3; BRD3_HUMAN; Bromodomain containing 3; Bromodomain containing protein 3; Bromodomain-containing protein 3; KIAA0043; Open reading frame X; ORFX; RING3 like gene; RING3-like protein; RING3L;

Immunogens

Immunogen:
Uniprot:

Q15059(BRD3_HUMAN) >>Visit HPA database.

Gene(ID):
BRD3(8019)
Expression:
Q15059 BRD3_HUMAN:

Ubiquitous.

Sequence:
MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPVEAPALPAPAAPMVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLKAVHEQLAALSQAPVNKPKKKKEKKEKEKKKKDKEKEKEKHKVKAEEEKKAKVAPPAKQAQQKKAPAKKANSTTTAGRQLKKGGKQASASYDSEEEEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQSREPSLRDSNPDEIEIDFETLKPTTLRELERYVKSCLQKKQRKPFSASGKKQAAKSKEELAQEKKKELEKRLQDVSGQLSSSKKPARKEKPGSAPSGGPSRLSSSSSSESGSSSSSGSSSDSSDSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Pig
91
Bovine
91
Dog
91
Xenopus
91
Rabbit
91
Horse
82
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15059 As Substrate

Site PTM Type Enzyme
S2 Acetylation
T15 Phosphorylation
K75 Ubiquitination
T85 Phosphorylation
T250 Phosphorylation
S254 Phosphorylation
T257 Phosphorylation
S259 Phosphorylation
S261 Phosphorylation
S263 Phosphorylation
S268 Phosphorylation
K274 Ubiquitination
S281 Phosphorylation
K304 Acetylation
K304 Methylation
K305 Acetylation
K305 Methylation
K307 Acetylation
K362 Ubiquitination
R411 Methylation
S481 Phosphorylation
K539 Ubiquitination
S558 Phosphorylation
S560 Phosphorylation
S563 Phosphorylation
K591 Ubiquitination
S609 Phosphorylation
K655 Acetylation
K666 Acetylation
S676 Phosphorylation
S680 Phosphorylation
S681 Phosphorylation
S682 Phosphorylation
S703 Phosphorylation
S705 Phosphorylation
S706 Phosphorylation
S707 Phosphorylation
S708 Phosphorylation
S710 Phosphorylation
S712 Phosphorylation
S716 Phosphorylation
S718 Phosphorylation
S719 Phosphorylation
S720 Phosphorylation
S722 Phosphorylation
S723 Phosphorylation
S725 Phosphorylation

Research Backgrounds

Function:

Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).

Subcellular Location:

Nucleus.
Note: Detected on chromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts (via bromo domain 1) with GATA1 acetylated at 'Lys-312' and 'Lys-315'. Interacts (via bromo domain 1) with GATA2 acetylated on lysine residues (By similarity). Interacts (via bromo domains) with acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (in vitro).

Family&Domains:

The Bromo domains specifically recognize and bind acetylated histones.

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