CAS Antibody - #AF9032

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Product: CAS Antibody
Catalog: AF9032
Description: Rabbit polyclonal antibody to CAS
Application: WB IF/ICC
Reactivity: Human, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 110kDa; 110kD(Calculated).
Uniprot: P55060
RRID: AB_2843223

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
CAS Antibody detects endogenous levels of total CAS.
RRID:
AB_2843223
Cite Format: Affinity Biosciences Cat# AF9032, RRID:AB_2843223.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAS; Cellular apoptosis susceptibility protein; Chromosome segregation 1 (yeast homolog) like; Chromosome segregation 1 Like; Chromosome segregation 1 like protein; Chromosome segregation 1-like protein; Chromosome segregation gene CSE1; CSE 1; CSE 1 chromosome segregation 1 like; CSE 1 chromosome segregation 1 like protein; CSE 1L; CSE1; CSE1 chromosome segregation 1 like (yeast); CSE1 chromosome segregation 1 like; CSE1 chromosome segregation 1 like protein; CSE1L; Exp 2; Exp2; Exportin 2; Exportin-2; Exportin2; Importin alpha re exporter; Importin-alpha re-exporter; MGC117283; MGC130036; MGC130037; XPO 2; XPO2; XPO2_HUMAN;

Immunogens

Immunogen:
Uniprot:

P55060(XPO2_HUMAN) >>Visit HPA database.

Gene(ID):
CSE1L(1434)
Expression:
P55060 XPO2_HUMAN:

Detected in brain, placenta, ovary, testis and trachea (at protein level) (PubMed:10331944). Widely expressed (PubMed:10331944). Highly expressed in testis and in proliferating cells (PubMed:7479798,PubMed:10331944).

Sequence:
MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYIKRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQSGDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHANDASALRILFSSLILISKLFYSLNFQDLPEFFEDNMETWMNNFHTLLTLDNKLLQTDDEEEAGLLELLKSQICDNAALYAQKYDEEFQRYLPRFVTAIWNLLVTTGQEVKYDLLVSNAIQFLASVCERPHYKNLFEDQNTLTSICEKVIVPNMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTGIFSGYVNSMLQEYAKNPSVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSANVNEFPVLKADGIKYIMIFRNQVPKEHLLVSIPLLINHLQAESIVVHTYAAHALERLFTMRGPNNATLFTAAEIAPFVEILLTNLFKALTLPGSSENEYIMKAIMRSFSLLQEAIIPYIPTLITQLTQKLLAVSKNPSKPHFNHYMFEAICLSIRITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55060 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K16 Ubiquitination
K17 Acetylation
K17 Ubiquitination
T18 Phosphorylation
K31 Ubiquitination
K52 Ubiquitination
K67 Ubiquitination
K71 Ubiquitination
R89 Methylation
S112 Phosphorylation
K158 Acetylation
K158 Ubiquitination
K165 Ubiquitination
S166 Phosphorylation
T185 Phosphorylation
T191 Phosphorylation
T255 Phosphorylation
K268 Ubiquitination
S269 Phosphorylation
C344 S-Nitrosylation
K346 Ubiquitination
R355 Methylation
S366 Phosphorylation
Y369 Phosphorylation
R371 Methylation
S377 Phosphorylation
T381 Phosphorylation
C387 S-Nitrosylation
Y408 Phosphorylation
K425 Ubiquitination
Y432 Phosphorylation
K440 Ubiquitination
K445 Ubiquitination
S471 Phosphorylation
K481 Ubiquitination
T562 Phosphorylation
S566 Phosphorylation
S567 Phosphorylation
Y571 Phosphorylation
K574 Acetylation
T698 Phosphorylation
K726 Ubiquitination
S789 Phosphorylation
Y797 Phosphorylation
K824 Acetylation
K824 Ubiquitination
K832 Ubiquitination
S834 Phosphorylation
K840 Ubiquitination
K848 Ubiquitination
S904 Phosphorylation
K912 Ubiquitination
K925 Ubiquitination
S931 Phosphorylation
K934 Ubiquitination
C939 S-Nitrosylation
S945 Phosphorylation

Research Backgrounds

Function:

Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Shuttles between the nucleus and the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain, placenta, ovary, testis and trachea (at protein level). Widely expressed. Highly expressed in testis and in proliferating cells.

Subunit Structure:

Found in a complex with CSE1L/XPO2, Ran and KPNA2. Binds with high affinity to importin-alpha only in the presence of RanGTP. The complex is dissociated by the combined action of RanBP1 and RanGAP1. Interacts with CFTR.

Family&Domains:

Belongs to the XPO2/CSE1 family.

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