Centrobin Antibody - #AF9040

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Product: Centrobin Antibody
Catalog: AF9040
Description: Rabbit polyclonal antibody to Centrobin
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 100kDa; 101kD(Calculated).
Uniprot: Q8N137
RRID: AB_2843231

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Centrobin Antibody detects endogenous levels of total Centrobin.
RRID:
AB_2843231
Cite Format: Affinity Biosciences Cat# AF9040, RRID:AB_2843231.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

9830165K03Rik; Centrobin; centrobin, centrosomal BRCA2 interacting protein; Centrosomal BRCA2 interacting protein; Centrosomal BRCA2-interacting protein; CNTRB_HUMAN; Cntrob; LIP8; LYST interacting protein 8; LYST-interacting protein 8; LYST-interacting protein LIP8; PP1221; RGD1307488; RP23 26L6.9;

Immunogens

Immunogen:
Uniprot:

Q8N137(CNTRB_HUMAN) >>Visit HPA database.

Gene(ID):
CNTROB(116840)
Expression:
Q8N137 CNTRB_HUMAN:

Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

Sequence:
MATSADSPSSPLGAEDLLSDSSEPPGLNQVSSEVTSQLYASLRLSRQAEATARAQLYLPSTSPPHEGLDGFAQELSRSLSVGLEKNLKKKDGSKHIFEMESVRGQLQTMLQTSRDTAYRDPLIPGAGSERREEDSFDSDSTATLLNTRPLQDLSPSSSAQALEELFPRYTSLRPGPPLNPPDFQGLRDALDSEHTRRKHCERHIQSLQTRVLELQQQLAVAVAADRKKDTMIEQLDKTLARVVEGWNRHEAERTEVLRGLQEEHQAAELTRSKQQETVTRLEQSLSEAMEALNREQESARLQQRERETLEEERQALTLRLEAEQQRCCVLQEERDAARAGQLSEHRELETLRAALEEERQTWAQQEHQLKEHYQALQEESQAQLEREKEKSQREAQAAWETQHQLALVQSEVRRLEGELDTARRERDALQLEMSLVQARYESQRIQLESELAVQLEQRVTERLAQAQESSLRQAASLREHHRKQLQDLSGQHQQELASQLAQFKVEMAEREERQQQVAEDYELRLAREQARVCELQSGNQQLEEQRVELVERLQAMLQAHWDEANQLLSTTLPPPNPPAPPAGPSSPGPQEPEKEERRVWTMPPMAVALKPVLQQSREARDELPGAPPVLCSSSSDLSLLLGPSFQSQHSFQPLEPKPDLTSSTAGAFSALGAFHPDHRAERPFPEEDPGPDGEGLLKQGLPPAQLEGLKNFLHQLLETVPQNNENPSVDLLPPKSGPLTVPSWEEAPQVPRIPPPVHKTKVPLAMASSLFRVPEPPSSHSQGSGPSSGSPERGGDGLTFPRQLMEVSQLLRLYQARGWGALPAEDLLLYLKRLEHSGTDGRGDNVPRRNTDSRLGEIPRKEIPSQAVPRRLATAPKTEKPPARKKSGHPAPSSMRSRGGVWR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8N137 As Substrate

Site PTM Type Enzyme
T35 Phosphorylation P51955 (NEK2)
S36 Phosphorylation P51955 (NEK2)
S41 Phosphorylation P51955 (NEK2)
S45 Phosphorylation P51955 (NEK2)
S62 Phosphorylation
S78 Phosphorylation
S80 Phosphorylation
K94 Ubiquitination
S128 Phosphorylation
Y169 Phosphorylation
S171 Phosphorylation
K227 Methylation
K228 Methylation
T230 Phosphorylation
K237 Ubiquitination
R258 Methylation
K504 Ubiquitination
K610 Ubiquitination
K698 Ubiquitination
S743 Phosphorylation
K761 Ubiquitination
S781 Phosphorylation
S790 Phosphorylation
Y814 Phosphorylation
K832 Ubiquitination
S837 Phosphorylation
K861 Ubiquitination
S887 Phosphorylation

Research Backgrounds

Function:

Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Centriole-associated, asymmetrically localizes to the daughter centriole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.

Subunit Structure:

Interacts with LYST.

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