CEP152 Antibody - #AF9041

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Product: CEP152 Antibody
Catalog: AF9041
Description: Rabbit polyclonal antibody to CEP152
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 140kDa; 196kD(Calculated).
Uniprot: O94986
RRID: AB_2843232

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(90%), Bovine(83%), Horse(91%), Sheep(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
CEP152 Antibody detects endogenous levels of total CEP152.
RRID:
AB_2843232
Cite Format: Affinity Biosciences Cat# AF9041, RRID:AB_2843232.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Asterless; CE152_HUMAN; Centrosomal protein 152kDa; Centrosomal protein of 152 kDa; Cep152; FLJ21594; KIAA0912; MCPH4; MCPH9; Microcephaly primary autosomal recessive 4; SCKL5;

Immunogens

Immunogen:
Uniprot:

O94986(CE152_HUMAN) >>Visit HPA database.

Gene(ID):
CEP152(22995)
Sequence:
MSLDFGSVALPVQNEDEEYDEEDYEREKELQQLLTDLPHDMLDDDLSSPELQYSDCSEDGTDGQPHHPEQLEMSWNEQMLPKSQSVNGYNEIQSLYAGEKCGNVWEENRSKTEDRHPVYHPEEGGDEGGSGYSPPSKCEQTDLYHLPENFRPYTNGQKQEFNNQATNVIKFSDPQWNHFQGPSCQGLEPYNKVTYKPYQSSAQNNGSPAQEITGSDTFEGLQQQFLGANENSAENMQIIQLQVLNKAKERQLENLIEKLNESERQIRYLNHQLVIIKDEKDGLTLSLRESQKLFQNGKEREIQLEAQIKALETQIQALKVNEEQMIKKSRTTEMALESLKQQLVDLHHSESLQRAREQHESIVMGLTKKYEEQVLSLQKNLDATVTALKEQEDICSRLKDHVKQLERNQEAIKLEKTEIINKLTRSLEESQKQCAHLLQSGSVQEVAQLQFQLQQAQKAHAMSANMNKALQEELTELKDEISLYESAAKLGIHPSDSEGELNIELTESYVDLGIKKVNWKKSKVTSIVQEEDPNEELSKDEFILKLKAEVQRLLGSNSMKRHLVSQLQNDLKDCHKKIEDLHQVKKDEKSIEVETKTDTSEKPKNQLWPESSTSDVVRDDILLLKNEIQVLQQQNQELKETEGKLRNTNQDLCNQMRQMVQDFDHDKQEAVDRCERTYQQHHEAMKTQIRESLLAKHALEKQQLFEAYERTHLQLRSELDKLNKEVTAVQECYLEVCREKDNLELTLRKTTEKEQQTQEKIKEKLIQQLEKEWQSKLDQTIKAMKKKTLDCGSQTDQVTTSDVISKKEMAIMIEEQKCTIQQNLEQEKDIAIKGAMKKLEIELELKHCENITKQVEIAVQNAHQRWLGELPELAEYQALVKAEQKKWEEQHEVSVNKRISFAVSEAKEKWKSELENMRKNILPGKELEEKIHSLQKELELKNEEVPVVIRAELAKARSEWNKEKQEEIHRIQEQNEQDYRQFLDDHRNKINEVLAAAKEDFMKQKTELLLQKETELQTCLDQSRREWTMQEAKRIQLEIYQYEEDILTVLGVLLSDTQKEHISDSEDKQLLEIMSTCSSKWMSVQYFEKLKGCIQKAFQDTLPLLVENADPEWKKRNMAELSKDSASQGTGQGDPGPAAGHHAQPLALQATEAEADKKKVLEIKDLCCGHCFQELEKAKQECQDLKGKLEKCCRHLQHLERKHKAVVEKIGEENNKVVEELIEENNDMKNKLEELQTLCKTPPRSLSAGAIENACLPCSGGALEELRGQYIKAVKKIKCDMLRYIQESKERAAEMVKAEVLRERQETARKMRKYYLICLQQILQDDGKEGAEKKIMNAASKLATMAKLLETPISSKSQSKTTQSALPLTSEMLIAVKKSKRNDVNQKIPCCIESKSNSVNTITRTLCEQAPKRRAACNLQRLLENSEHQSIKHVGSKETHLEFQFGDGSCKHLNSLPRNVSPEFVPCEGEGGFGLHKKKDLLSDNGSESLPHSAAYPFLGTLGNKPSPRCTPGPSESGCMHITFRDSNERLGLKVYKCNPLMESENAASEKSQGLDVQEPPVKDGGDLSDCLGWPSSSATLSFDSREASFVHGRPQGTLEIPSESVKSKQFSPSGYLSDTEESNMICQTMKCQRYQTPYLSEETTYLEPGKISVNCGHPSRHKADRLKSDFKKLSSTLPSSVCQQPSRKLIVPLSSQQDSGFDSPFVNLD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Pig
90
Bovine
83
Sheep
83
Dog
83
Rabbit
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94986 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
Y24 Phosphorylation
S85 Phosphorylation
Y89 Phosphorylation
K100 Ubiquitination
S133 Phosphorylation
Y144 Phosphorylation
K170 Ubiquitination
T367 Phosphorylation
K379 Ubiquitination
K389 Ubiquitination
K413 Ubiquitination
K416 Ubiquitination
T417 Phosphorylation
K422 Ubiquitination
K432 Ubiquitination
K458 Ubiquitination
K468 Ubiquitination
K478 Ubiquitination
S497 Phosphorylation
K523 Ubiquitination
K539 Ubiquitination
K545 Ubiquitination
K547 Ubiquitination
K560 Ubiquitination
K572 Ubiquitination
K577 Ubiquitination
K585 Ubiquitination
S590 Phosphorylation
T595 Phosphorylation
K596 Ubiquitination
K625 Ubiquitination
K639 Ubiquitination
K644 Ubiquitination
K667 Ubiquitination
T677 Phosphorylation
Y678 Phosphorylation
K686 Ubiquitination
K696 Ubiquitination
K701 Ubiquitination
K740 Ubiquitination
K764 Ubiquitination
K771 Ubiquitination
K776 Ubiquitination
K782 Ubiquitination
K787 Ubiquitination
S805 Phosphorylation
K806 Ubiquitination
K828 Ubiquitination
K833 Ubiquitination
K853 Ubiquitination
K881 Ubiquitination
K886 Ubiquitination
K897 Ubiquitination
S900 Phosphorylation
K907 Ubiquitination
K911 Ubiquitination
K919 Ubiquitination
K925 Ubiquitination
K930 Ubiquitination
K936 Ubiquitination
K941 Ubiquitination
K964 Ubiquitination
K989 Ubiquitination
K998 Ubiquitination
K1005 Ubiquitination
T1006 Phosphorylation
K1012 Ubiquitination
K1033 Ubiquitination
K1068 Ubiquitination
K1091 Ubiquitination
K1096 Ubiquitination
T1101 Phosphorylation
K1114 Ubiquitination
K1164 Ubiquitination
K1179 Ubiquitination
K1229 Ubiquitination
K1231 Ubiquitination
K1240 Ubiquitination
T1241 Phosphorylation
S1247 Phosphorylation
K1272 Acetylation
K1272 Ubiquitination
K1276 Acetylation
K1278 Acetylation
K1278 Ubiquitination
K1289 Ubiquitination
K1297 Ubiquitination
Y1314 Phosphorylation
K1328 Ubiquitination
K1341 Ubiquitination
K1347 Ubiquitination
T1351 Phosphorylation
K1356 Ubiquitination
K1360 Ubiquitination
K1377 Ubiquitination
K1387 Ubiquitination
K1395 Ubiquitination
K1412 Ubiquitination
S1426 Phosphorylation
S1430 Phosphorylation
K1432 Ubiquitination
K1437 Ubiquitination
K1451 Ubiquitination
S1455 Phosphorylation
S1461 Phosphorylation
K1477 Ubiquitination
K1505 Ubiquitination
S1507 Phosphorylation
K1534 Ubiquitination
K1537 Ubiquitination
S1552 Phosphorylation
K1563 Ubiquitination
S1589 Phosphorylation
T1598 Phosphorylation
K1607 Ubiquitination
S1618 Phosphorylation
K1631 Ubiquitination
Y1646 Phosphorylation
K1651 Ubiquitination
K1673 Ubiquitination
K1689 Ubiquitination

Research Backgrounds

Function:

Necessary for centrosome duplication; the function seems also to involve CEP63, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication. Acts as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, 2 molecules involved in centriole formation. Proposed to snatch PLK4 away from PLK4:CEP92 complexes in early G1 daughter centriole and to reposition PLK4 at the outer boundary of a newly forming CEP152 ring structure. Also plays a key role in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles (By similarity). Overexpression of CEP152 can drive amplification of centrioles.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Note: Colocalizes with CDK5RAP2, WDR62 and CEP63 in a discrete ring around the proximal end of the parental centriole. At this site, a cohesive structure is predicted to engage parental centrioles and procentrioles (PubMed:21983783, PubMed:26297806). Localizes to the deuterosome (By similarity). Localizes to pericentriolar material (PCM) (PubMed:26337392).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via N-terminus) with PLK4; the interaction is mutally exclusive with a PLK4:CEP192 interaction. Interacts (via C-terminus) with CENPJ (via-N-terminus). Interacts with CINP. Interacts with CDK5RAP2, WDR62, CEP63 and CEP131. CEP63, CDK5RAP2, CEP152, WDR62 are proposed to form a stepwise assembled complex at the centrosome forming a ring near parental centrioles. Interacts with DEUP1; this interaction recruits CEP152 to the deuterosome. The interactions with CEP63 and DEUP1 are mutually exclusive (By similarity).

Family&Domains:

Belongs to the CEP152 family.

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