Product: DHHC-9 Antibody
Catalog: AF9054
Description: Rabbit polyclonal antibody to DHHC-9
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit
Mol.Wt.: 41kDa; 41kD(Calculated).
Uniprot: Q9Y397
RRID: AB_2843245

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(86%)
Clonality:
Polyclonal
Specificity:
DHHC-9 Antibody detects endogenous levels of total DHHC-9.
RRID:
AB_2843245
Cite Format: Affinity Biosciences Cat# AF9054, RRID:AB_2843245.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen MMSA 1; Asp His His Cys domain containing protein 9; CGI89; CXorf11; DHHC-9; DHHC9; MMSA1; MRXSZ; Palmitoyltransferase ZDHHC9; ZDHC9_HUMAN; ZDHHC 9; ZDHHC10; ZDHHC9; Zinc finger DHHC domain containing 10; Zinc finger DHHC domain containing protein 9; Zinc finger DHHC domain-containing protein 9; Zinc finger DHHC type containing 9; Zinc finger protein 379; Zinc finger protein 380; ZNF379; ZNF380;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9Y397 ZDHC9_HUMAN:

Highly expressed in kidney, skeletal muscle, brain, lung and liver. Absent in thymus, spleen and leukocytes.

Sequence:
MSVMVVRKKVTRKWEKLPGRNTFCCDGRVMMARQKGIFYLTLFLILGTCTLFFAFECRYLAVQLSPAIPVFAAMLFLFSMATLLRTSFSDPGVIPRALPDEAAFIEMEIEATNGAVPQGQRPPPRIKNFQINNQIVKLKYCYTCKIFRPPRASHCSICDNCVERFDHHCPWVGNCVGKRNYRYFYLFILSLSLLTIYVFAFNIVYVALKSLKIGFLETLKETPGTVLEVLICFFTLWSVVGLTGFHTFLVALNQTTNEDIKGSWTGKNRVQNPYSHGNIVKNCCEVLCGPLPPSVLDRRGILPLEESGSRPPSTQETSSSLLPQSPAPTEHLNSNEMPEDSSTPEEMPPPEPPEPPQEAAEAEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Rabbit
86
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y397 As Substrate

Site PTM Type Enzyme
S79 Phosphorylation
T82 Phosphorylation
K127 Ubiquitination
K137 Ubiquitination
K267 Ubiquitination
K281 Ubiquitination

Research Backgrounds

Function:

The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in kidney, skeletal muscle, brain, lung and liver. Absent in thymus, spleen and leukocytes.

Subunit Structure:

Interacts with GOLGA7.

Family&Domains:

The DHHC domain is required for palmitoyltransferase activity.

Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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