EF-Tu Antibody | Affinity Biosciences

WB
Cites

1/2

EF-Tu Antibody - Western blot analysis of extracts from various samples, using EF-Tu Antibody.

EF-Tu Antibody - Figure 9 Effects of DOP treatment on gut–brain mediators.

Product:	EF-Tu Antibody
Catalog:	AF9057
Description:	Rabbit polyclonal antibody to EF-Tu
Application:	WB
Cited expt.:
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.:	40-50kD; 50kD(Calculated).
Uniprot:	P49411
RRID:	AB_2843248

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

EF-Tu Antibody detects endogenous levels of total EF-Tu.

RRID:

AB_2843248
Cite Format: Affinity Biosciences Cat# AF9057, RRID:AB_2843248.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COXPD4; EF Tu; EF TuMT; EF-Tu; EF-TuMT; EFTu; EFTU_HUMAN; EFTuMT; Elongation factor Tu; Elongation factor Tu mitochondrial; mitochondrial; P43; Tu translation elongation factor, mitochondrial; Tufm;

Immunogens

Immunogen:

A synthesized peptide derived from human EF-Tu, corresponding to a region within the internal amino acids.

Uniprot:

P49411(EFTU_HUMAN) >>Visit HPA database.

Gene(ID):

TUFM(7284)

Sequence:

P49411 EFTU_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Zebrafish

100

Chicken

100

Rabbit

100

Dog

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P49411_EFTU_HUMAN

Function:

Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.

Subcellular Location:

Mitochondrion.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

References

1). Dendrobium officinale polysaccharide ameliorates polycystic ovary syndrome via regulating butyrate dependent gut–brain–ovary axis mechanism. Frontiers in Endocrinology, 2023 (PubMed: 35992123) [IF=3.9]

Application: IHC Species: Rat Sample:

Figure 9 Effects of DOP treatment on gut–brain mediators. (A) Serum levels of PYY, n = 5. (B-C) Colonic GPR41 expression, location, and integrated optical density, n = 3, scale bar = 50 and 25 μm. Note: compared with the PCOS group, **p < 0.01, ***p < 0.001; compared with the NS group, #p < 0.05, ##p < 0.01, ###p < 0.001.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

EF-Tu Antibody - #AF9057