EF-Tu Antibody | Affinity Biosciences

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EF-Tu Antibody - Western blot analysis of extracts from various samples, using EF-Tu Antibody.

EF-Tu Antibody - Figure 9 Effects of DOP treatment on gut–brain mediators.

Product:	EF-Tu Antibody
Catalog:	AF9057
Description:	Rabbit polyclonal antibody to EF-Tu
Application:	WB
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.:	50kDa; 50kD(Calculated).
Uniprot:	P49411
RRID:	AB_2843248

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

EF-Tu Antibody detects endogenous levels of total EF-Tu.

RRID:

AB_2843248
Cite Format: Affinity Biosciences Cat# AF9057, RRID:AB_2843248.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COXPD4; EF Tu; EF TuMT; EF-Tu; EF-TuMT; EFTu; EFTU_HUMAN; EFTuMT; Elongation factor Tu; Elongation factor Tu mitochondrial; mitochondrial; P43; Tu translation elongation factor, mitochondrial; Tufm;

Immunogens

Immunogen:

Uniprot:

P49411(EFTU_HUMAN) >>Visit HPA database.

Gene(ID):

TUFM(7284)

Sequence:

P49411 EFTU_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Zebrafish

100

Chicken

100

Rabbit

100

Dog

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49411 As Substrate

P49411

Site	PTM Type	Source
K32	Ubiquitination	Uniprot
K48	Ubiquitination	Uniprot
K55	Ubiquitination	Uniprot
T62	Phosphorylation	Uniprot
T74	Phosphorylation	Uniprot
K79	Acetylation	Uniprot
K79	Ubiquitination	Uniprot
K88	Acetylation	Uniprot
K88	Ubiquitination	Uniprot
K91	Acetylation	Uniprot
K91	Methylation	Uniprot
K91	Ubiquitination	Uniprot
Y92	Phosphorylation	Uniprot
R102	Methylation	Uniprot
R104	Methylation	Uniprot
Y115	Phosphorylation	Uniprot
C127	S-Nitrosylation	Uniprot
Y133	Phosphorylation	Uniprot
Y179	Phosphorylation	Uniprot
T204	Phosphorylation	Uniprot
Y208	Phosphorylation	Uniprot
R227	Methylation	Uniprot
K234	Ubiquitination	Uniprot
K238	Acetylation	Uniprot
K238	Ubiquitination	Uniprot
T245	Phosphorylation	Uniprot
Y246	Phosphorylation	Uniprot
K256	Acetylation	Uniprot
K256	Ubiquitination	Uniprot
Y266	Phosphorylation	Uniprot
S267	Phosphorylation	Uniprot
T278	Phosphorylation	Uniprot
R281	Methylation	Uniprot
K286	Ubiquitination	Uniprot
C290	S-Nitrosylation	Uniprot
S296	Phosphorylation	Uniprot
K297	Ubiquitination	Uniprot
R300	Methylation	Uniprot
T304	Phosphorylation	Uniprot
K311	Acetylation	Uniprot
K311	Ubiquitination	Uniprot
S312	Phosphorylation	Uniprot
K342	Ubiquitination	Uniprot
K351	Acetylation	Uniprot
Y357	Phosphorylation	Uniprot
S360	Phosphorylation	Uniprot
K361	Acetylation	Uniprot
K361	Ubiquitination	Uniprot
T382	Phosphorylation	Uniprot
K405	Acetylation	Uniprot
K418	Acetylation	Uniprot
K418	Ubiquitination	Uniprot
T443	Phosphorylation	Uniprot
K447	Acetylation	Uniprot
K447	Ubiquitination	Uniprot

Research Backgrounds

P49411_EFTU_HUMAN

Function:

Promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays also a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIG-I/DDX58-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.

Subcellular Location:

Mitochondrion.

Subunit Structure:

Interacts with NLRX1. Interacts with ATG16L1.

(Microbial infection) Interacts with human parainfluenza virus 3 matrix protein; this interaction inhibits RLR-mediated type I interferon production while promoting autophagy.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

References

1). Dendrobium officinale polysaccharide ameliorates polycystic ovary syndrome via regulating butyrate dependent gut–brain–ovary axis mechanism. Frontiers in Endocrinology, 2023 (PubMed: 35992123) [IF=3.9]

Application: IHC Species: Rat Sample:

Figure 9 Effects of DOP treatment on gut–brain mediators. (A) Serum levels of PYY, n = 5. (B-C) Colonic GPR41 expression, location, and integrated optical density, n = 3, scale bar = 50 and 25 μm. Note: compared with the PCOS group, **p < 0.01, ***p < 0.001; compared with the NS group, #p < 0.05, ##p < 0.01, ###p < 0.001.

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

EF-Tu Antibody - #AF9057