Elongin A1 Antibody - #AF9059

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Product: Elongin A1 Antibody
Catalog: AF9059
Description: Rabbit polyclonal antibody to Elongin A1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 87kDa; 90kD(Calculated).
Uniprot: Q14241
RRID: AB_2843250

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(91%), Horse(91%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
Elongin A1 Antibody detects endogenous levels of total Elongin A1.
RRID:
AB_2843250
Cite Format: Affinity Biosciences Cat# AF9059, RRID:AB_2843250.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ela1; Elo A; EloA; ELOA1_HUMAN; Elongin 110 kDa subunit; Elongin-A; ElonginA; RNA polymerase II transcription factor SIII subunit A1; SIII; SIII p110; TCEB 3; Tceb3; TCEB3A; Transcription elongation factor B (SIII) polypeptide 3 (110kD); Transcription elongation factor B (SIII) polypeptide 3; Transcription elongation factor B alpha subunit; Transcription elongation factor B polypeptide 3;

Immunogens

Immunogen:
Uniprot:

Q14241(ELOA1_HUMAN) >>Visit HPA database.

Gene(ID):
ELOA(6924)
Sequence:
MHGGRSCGPRTRREPSSGEEAAPVTAMAAESALQVVEKLQARLAANPDPKKLLKYLKKLSTLPITVDILAETGVGKTVNSLRKHEHVGSFARDLVAQWKKLVPVERNAEPDEQDFEKSNSRKRPRDALQKEEEMEGDYQETWKATGSRSYSPDHRQKKHRKLSELERPHKVSHGHERRDERKRCHRMSPTYSSDPESSDYGHVQSPPSCTSPHQMYVDHYRSLEEDQEPIVSHQKPGKGHSNAFQDRLGASQERHLGEPHGKGVVSQNKEHKSSHKDKRPVDAKSDEKASVVSREKSHKALSKEENRRPPSGDNAREKPPSSGVKKEKDREGSSLKKKCLPPSEAASDNHLKKPKHRDPEKAKLDKSKQGLDSFDTGKGAGDLLPKVKEKGSNNLKTPEGKVKTNLDRKSLGSLPKVEETDMEDEFEQPTMSFESYLSYDQPRKKKKKIVKTSATALGDKGLKKNDSKSTGKNLDSVQKLPKVNKTKSEKPAGADLAKLRKVPDVLPVLPDLPLPAIQANYRPLPSLELISSFQPKRKAFSSPQEEEEAGFTGRRMNSKMQVYSGSKCAYLPKMMTLHQQCIRVLKNNIDSIFEVGGVPYSVLEPVLERCTPDQLYRIEEYNHVLIEETDQLWKVHCHRDFKEERPEEYESWREMYLRLQDAREQRLRVLTKNIQFAHANKPKGRQAKMAFVNSVAKPPRDVRRRQEKFGTGGAAVPEKIKIKPAPYPMGSSHASASSISFNPSPEEPAYDGPSTSSAHLAPVVSSTVSYDPRKPTVKKIAPMMAKTIKAFKNRFSRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
91
Bovine
91
Dog
91
Rabbit
91
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14241 As Substrate

Site PTM Type Enzyme
S31 Phosphorylation
K38 Methylation
T72 Phosphorylation
K83 Ubiquitination
S118 Phosphorylation
Y138 Phosphorylation
T141 Phosphorylation
S147 Phosphorylation
S151 Phosphorylation
S163 Phosphorylation
K170 Ubiquitination
S188 Phosphorylation
T190 Phosphorylation
S193 Phosphorylation
S205 Phosphorylation
S208 Phosphorylation
S211 Phosphorylation
Y220 Phosphorylation
S222 Phosphorylation
S232 Phosphorylation
K235 Ubiquitination
R247 Methylation
S251 Phosphorylation
K262 Ubiquitination
S311 Phosphorylation
S343 Phosphorylation
S347 Phosphorylation
S373 Phosphorylation
T397 Phosphorylation
S410 Phosphorylation
S413 Phosphorylation
T420 Phosphorylation
K472 Acetylation
S476 Phosphorylation
K479 Ubiquitination
K538 Ubiquitination
S541 Phosphorylation
S542 Phosphorylation
T552 Phosphorylation
Y563 Phosphorylation
S564 Phosphorylation
K681 Acetylation
T711 Phosphorylation

Research Backgrounds

Function:

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotrimer of an A (ELOA, ELOA2 or ELOA3), ELOB and ELOC subunit (By similarity). Interacts with ERCC6; the interaction is induced by DNA damaging agents or inhibitors of RNA polymerase II elongation (By similarity). Interacts (via BC-box) with CUL5 (By similarity).

Family&Domains:

The BC-box, which mediates binding to the elongin BC complex, has the consensus sequence [APST]-L-x(3)-C-x(3)-[AILV].

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