ETL Antibody | Affinity Biosciences

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ETL Antibody - Western blot analysis of ETL using JK whole cell lysates.

Product:	ETL Antibody
Catalog:	AF9063
Description:	Rabbit polyclonal antibody to ETL
Application:	WB IHC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	77kDa; 78kD(Calculated).
Uniprot:	Q9HBW9
RRID:	AB_2843254

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

ETL Antibody detects endogenous levels of total ETL.

RRID:

AB_2843254
Cite Format: Affinity Biosciences Cat# AF9063, RRID:AB_2843254.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADGRL4; Adhesion G protein-coupled receptor L4; EGF; EGF latrophilin and seven transmembrane domain containing 1; EGF TM7 latrophilin related protein; EGF, latrophilin and seven transmembrane domain-containing protein 1; EGF, latrophilin, and 7 transmembrane domain-containing protein 1; EGF-, TM7-, and latrophilin-related protein; EGF-TM7-latrophilin-related protein; EGFTM7 latrophilin related protein; ELTD 1; ELTD1; ELTD1_HUMAN; ETL; ETL protein; KPG_003; latrophilin and seven transmembrane domain-containing protein 1; UNQ202/PRO228; zgc:63629;

Immunogens

Immunogen:

Uniprot:

Q9HBW9(AGRL4_HUMAN) >>Visit HPA database.

Gene(ID):

ADGRL4(64123)

Expression:

Q9HBW9 AGRL4_HUMAN:

Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.

Sequence:

Q9HBW9 AGRL4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKRLPLLVVFSTLLNCSYTQNCTKTPCLPNAKCEIRNGIEACYCNMGFSGNGVTICEDDNECGNLTQSCGENANCTNTEGSYYCMCVPGFRSSSNQDRFITNDGTVCIENVNANCHLDNVCIAANINKTLTKIRSIKEPVALLQEVYRNSVTDLSPTDIITYIEILAESSSLLGYKNNTISAKDTLSNSTLTEFVKTVNNFVQRDTFVVWDKLSVNHRRTHLTKLMHTVEQATLRISQSFQKTTEFDTNSTDIALKVFFFDSYNMKHIHPHMNMDGDYINIFPKRKAAYDSNGNVAVAFVYYKSIGPLLSSSDNFLLKPQNYDNSEEEERVISSVISVSMSSNPPTLYELEKITFTLSHRKVTDRYRSLCAFWNYSPDTMNGSWSSEGCELTYSNETHTSCRCNHLTHFAILMSSGPSIGIKDYNILTRITQLGIIISLICLAICIFTFWFFSEIQSTRTTIHKNLCCSLFLAELVFLVGINTNTNKLFCSIIAGLLHYFFLAAFAWMCIEGIHLYLIVVGVIYNKGFLHKNFYIFGYLSPAVVVGFSAALGYRYYGTTKVCWLSTENNFIWSFIGPACLIILVNLLAFGVIIYKVFRHTAGLKPEVSCFENIRSCARGALALLFLLGTTWIFGVLHVVHASVVTAYLFTVSNAFQGMFIFLFLCVLSRKIQEEYYRLFKNVPCCFGCLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

Rabbit

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBW9 As Substrate

Q9HBW9

Site	PTM Type	Source
T220	Phosphorylation	Uniprot
T223	Phosphorylation	Uniprot
N249	N-Glycosylation	Uniprot
Y301	Phosphorylation	Uniprot
Y302	Phosphorylation	Uniprot
T407	Phosphorylation	Uniprot

Research Backgrounds

Q9HBW9_AGRL4_HUMAN

Function:

Endothelial orphan receptor that acts as a key regulator of angiogenesis.

PTMs:

Glycosylated.

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Detected in the majority of epithelial cells in tumor and normal tissues. Expressed also in human umbilical vein endothelial cells.

Subunit Structure:

Heterodimer of 2 chains generated by proteolytic processing; the large extracellular N-terminal fragment and the membrane-bound C-terminal fragment predominantly remain associated and non-covalently linked.

Family&Domains:

The transmembrane domain is not required for cleavage, but it is required for dimer formation.

Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ETL Antibody - #AF9063