Product: FBP3 Antibody
Catalog: AF9065
Description: Rabbit polyclonal antibody to FBP3
Application: WB IF/ICC
Cited expt.:
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Dog, Chicken
Mol.Wt.: 60kDa; 62kD(Calculated).
Uniprot: Q96I24
RRID: AB_2843256

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(92%), Sheep(83%), Dog(92%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
FBP3 Antibody detects endogenous levels of total FBP3.
RRID:
AB_2843256
Cite Format: Affinity Biosciences Cat# AF9065, RRID:AB_2843256.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Far upstream element (FUSE) binding protein 3; Far upstream element binding protein 3; Far upstream element-binding protein 3; FBP3; FLJ25229; FUBP 3; FUBP3; FUBP3_HUMAN; FUSE binding protein 3; FUSE-binding protein 3; OTTHUMP00000022369;

Immunogens

Immunogen:

A synthesized peptide derived from human FBP3, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q96I24 FUBP3_HUMAN:

Detected in a number of cell lines.

Sequence:
MAELVQGQSAPVGMKAEGFVDALHRVRQIAAKIDSIPHLNNSTPLVDPSVYGYGVQKRPLDDGVGNQLGALVHQRTVITEEFKVPDKMVGFIIGRGGEQISRIQAESGCKIQIASESSGIPERPCVLTGTPESIEQAKRLLGQIVDRCRNGPGFHNDIDSNSTIQEILIPASKVGLVIGRGGETIKQLQERTGVKMVMIQDGPLPTGADKPLRITGDAFKVQQAREMVLEIIREKDQADFRGVRGDFNSRMGGGSIEVSVPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGISPERAAQVMGPPDRCQHAAHIISELILTAQERDGFGGLAAARGRGRGRGDWSVGAPGGVQEITYTVPADKCGLVIGKGGENIKSINQQSGAHVELQRNPPPNSDPNLRRFTIRGVPQQIEVARQLIDEKVGGTNLGAPGAFGQSPFSQPPAPPHQNTFPPRSSGCFPNMAAKVNGNPHSTPVSGPPAFLTQGWGSTYQAWQQPTQQVPSQQSQPQSSQPNYSKAWEDYYKKQSHAASAAPQASSPPDYTMAWAEYYRQQVAFYGQTLGQAQAHSQEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Chicken
100
Horse
92
Dog
92
Sheep
83
Bovine
75
Xenopus
55
Zebrafish
50
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in a number of cell lines.

References

1). Identification of a key glioblastoma candidate gene, FUBP3, based on weighted gene co-expression network analysis. BMC Neurology, 2022 (PubMed: 35413821) [IF=2.6]

Application: IHC    Species: Human    Sample: GBM tissues

Fig. 4A The expression of FUBP3 in normal tissues adjacent to cancer. B The expression of FUBP3 in GBM tissues. C The expression of CD4 + T cell in GBM tissues. D The expression of CD4 + T cell in normal tissues adjacent to cancer. E The expression of CD8 + T cell in GBM tissues. F The expression of CD8 + T cell in normal tissues adjacent to cancer. G The expression of CD68 + macrophages in GBM tissues. H The expression of CD68 + macrophages in normal tissues adjacent to cancer

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.