FBP3 Antibody - #AF9065
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| Product: | FBP3 Antibody |
| Catalog: | AF9065 |
| Description: | Rabbit polyclonal antibody to FBP3 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Horse, Sheep, Dog, Chicken |
| Mol.Wt.: | 60kDa; 62kD(Calculated). |
| Uniprot: | Q96I24 |
| RRID: | AB_2843256 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(92%), Sheep(83%), Dog(92%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
FBP3 Antibody detects endogenous levels of total FBP3.
RRID:
AB_2843256
Cite Format: Affinity Biosciences Cat# AF9065, RRID:AB_2843256.
Cite Format: Affinity Biosciences Cat# AF9065, RRID:AB_2843256.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Far upstream element (FUSE) binding protein 3; Far upstream element binding protein 3; Far upstream element-binding protein 3; FBP3; FLJ25229; FUBP 3; FUBP3; FUBP3_HUMAN; FUSE binding protein 3; FUSE-binding protein 3; OTTHUMP00000022369;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q96I24 FUBP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELVQGQSAPVGMKAEGFVDALHRVRQIAAKIDSIPHLNNSTPLVDPSVYGYGVQKRPLDDGVGNQLGALVHQRTVITEEFKVPDKMVGFIIGRGGEQISRIQAESGCKIQIASESSGIPERPCVLTGTPESIEQAKRLLGQIVDRCRNGPGFHNDIDSNSTIQEILIPASKVGLVIGRGGETIKQLQERTGVKMVMIQDGPLPTGADKPLRITGDAFKVQQAREMVLEIIREKDQADFRGVRGDFNSRMGGGSIEVSVPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGISPERAAQVMGPPDRCQHAAHIISELILTAQERDGFGGLAAARGRGRGRGDWSVGAPGGVQEITYTVPADKCGLVIGKGGENIKSINQQSGAHVELQRNPPPNSDPNLRRFTIRGVPQQIEVARQLIDEKVGGTNLGAPGAFGQSPFSQPPAPPHQNTFPPRSSGCFPNMAAKVNGNPHSTPVSGPPAFLTQGWGSTYQAWQQPTQQVPSQQSQPQSSQPNYSKAWEDYYKKQSHAASAAPQASSPPDYTMAWAEYYRQQVAFYGQTLGQAQAHSQEQ
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Chicken
100
Horse
92
Dog
92
Sheep
83
Bovine
75
Xenopus
55
Zebrafish
50
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96I24 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Sumoylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| Y51 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T76 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| R95 | Methylation | Uniprot | |
| C109 | S-Nitrosylation | Uniprot | |
| T130 | Phosphorylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| R147 | Methylation | Uniprot | |
| R149 | Methylation | Uniprot | |
| R180 | Methylation | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| R241 | Methylation | Uniprot | |
| R244 | Methylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| R286 | Methylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| R299 | Methylation | Uniprot | |
| R309 | Methylation | Uniprot | |
| R337 | Methylation | Uniprot | |
| R339 | Methylation | Uniprot | |
| R341 | Methylation | Uniprot | |
| R343 | Methylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| T360 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| R408 | Methylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| S457 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| K518 | Sumoylation | Uniprot | |
| Y523 | Phosphorylation | Uniprot | |
| K525 | Ubiquitination | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| S532 | Phosphorylation | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| Y543 | Phosphorylation | Uniprot | |
| T544 | Phosphorylation | Uniprot | |
| Y550 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression.
Subcellular Location:
Nucleus.
Tissue Specificity:
Detected in a number of cell lines.
References
1). Identification of a key glioblastoma candidate gene, FUBP3, based on weighted gene co-expression network analysis. BMC Neurology, 2022
(PubMed: 35413821)
[IF=2.6]
Application: IHC Species: Human Sample: GBM tissues
Fig. 4A The expression of FUBP3 in normal tissues adjacent to cancer. B The expression of FUBP3 in GBM tissues. C The expression of CD4 + T cell in GBM tissues. D The expression of CD4 + T cell in normal tissues adjacent to cancer. E The expression of CD8 + T cell in GBM tissues. F The expression of CD8 + T cell in normal tissues adjacent to cancer. G The expression of CD68 + macrophages in GBM tissues. H The expression of CD68 + macrophages in normal tissues adjacent to cancer
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