CD171/N-CAML1 Antibody - #AF9123
| Product: | CD171/N-CAML1 Antibody |
| Catalog: | AF9123 |
| Description: | Rabbit polyclonal antibody to CD171/N-CAML1 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 180kDa; 140kD(Calculated). |
| Uniprot: | P32004 |
| RRID: | AB_2843313 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9123, RRID:AB_2843313.
Fold/Unfold
Antigen identified by monoclonal antibody R1; CAML1; CD171; CD171 antigen; HSAS; HSAS1; Hyd; L1; L1 cell adhesion molecule; L1-NCAM; L1cam; L1CAM_HUMAN; MASA; MIC5; N CAML1; N-CAM-L1; NCAM-L1; NCAML1; Nerve-growth factor-inducible large external glycoprotein; Neural cell adhesion molecule L1; NILE; OTTHUMP00000025992; S10; SPG1;
Immunogens
- P32004 L1CAM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVVALRYVWPLLLCSPCLLIQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATEGWFIGFVSAIILLLLVLLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAFGSSQPSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P32004 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| K56 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| Y418 | Phosphorylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| T518 | Phosphorylation | Uniprot | |
| T521 | Phosphorylation | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| K530 | Acetylation | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| K661 | Ubiquitination | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| N671 | N-Glycosylation | Uniprot | |
| K694 | Ubiquitination | Uniprot | |
| K716 | Ubiquitination | Uniprot | |
| Y784 | Phosphorylation | Uniprot | |
| K998 | Ubiquitination | Uniprot | |
| K1150 | Ubiquitination | Uniprot | |
| Y1151 | Phosphorylation | Uniprot | |
| S1152 | Phosphorylation | Uniprot | |
| K1154 | Ubiquitination | Uniprot | |
| K1156 | Ubiquitination | Uniprot | |
| S1163 | Phosphorylation | Uniprot | |
| K1169 | Ubiquitination | Uniprot | |
| T1172 | Phosphorylation | P68400 (CSNK2A1) , P17252 (PRKCA) | Uniprot |
| Y1176 | Phosphorylation | P12931 (SRC) | Uniprot |
| S1181 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S1190 | Phosphorylation | Uniprot | |
| S1191 | Phosphorylation | Uniprot | |
| S1194 | Phosphorylation | Uniprot | |
| S1204 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| Y1211 | Phosphorylation | Uniprot | |
| Y1229 | Phosphorylation | P54753 (EPHB3) | Uniprot |
| K1235 | Ubiquitination | Uniprot | |
| S1243 | Phosphorylation | Uniprot | |
| S1244 | Phosphorylation | Uniprot | |
| T1247 | Phosphorylation | Uniprot | |
| S1248 | Phosphorylation | P28482 (MAPK1) | Uniprot |
Research Backgrounds
Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
Cell membrane>Single-pass type I membrane protein. Cell projection>Growth cone. Cell projection>Axon. Cell projection>Dendrite.
Note: Colocalized with SHTN1 in close apposition with actin filaments in filopodia and lamellipodia of axonalne growth cones of hippocampal neurons (By similarity). In neurons, detected predominantly in axons and cell body, weak localization to dendrites (PubMed:20621658).
Interacts with SHTN1; the interaction occurs in axonal growth cones.
Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Research Fields
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
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