Nek9 Antibody - #AF9125

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Product: Nek9 Antibody
Catalog: AF9125
Description: Rabbit polyclonal antibody to Nek9
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 107kDa; 107kD(Calculated).
Uniprot: Q8TD19
RRID: AB_2843315

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Nek9 Antibody detects endogenous levels of total Nek9.
RRID:
AB_2843315
Cite Format: Affinity Biosciences Cat# AF9125, RRID:AB_2843315.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA1995; APUG; DKFZp434D0935; LCCS10; MGC138306; MGC16714; NC; NEK 9; Nek8; nek9; NEK9_HUMAN; Nercc 1 kinase; NERCC; NERCC1; Nercc1 kinase; Never in mitosis A related kinase 9; Never in mitosis A-related kinase 9; NIMA (never in mitosis gene a) related kinase 9; NIMA related kinase 8; NIMA related kinase 9; NIMA related kinase Nek8; NimA related protein kinase 9; NIMA-related kinase 8; NimA-related protein kinase 9; Serine/threonine protein kinase Nek9; Serine/threonine-protein kinase Nek9;

Immunogens

Immunogen:
Uniprot:

Q8TD19(NEK9_HUMAN) >>Visit HPA database.

Gene(ID):
NEK9(91754)
Expression:
Q8TD19 NEK9_HUMAN:

Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

Sequence:
MSVLGEYERHCDSINSDFGSESGGCGDSSPGPSASQGPRAGGGAAEQEELHYIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQMVHSCLDQDPEQRPTADELLDRPLLRKRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIRQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVAGPEVLEPMQLNFFLSNPVEQVSCGDNHVVVLTRNKEVYSWGCGEYGRLGLDSEEDYYTPQKVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNEFNKLGLNQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGLSIGTVFQSSSPGGGGGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAMGNSNGASSSCPGWLRKELENAEFIPMPDSPSPLSAAFSESEKDTLPYEELQGLKVASEAPLEHKPQVEASSPRLNPAVTCAGKGTPLTPPACACSSLQVEVERLQGLVLKCLAEQQKLQQENLQIFTQLQKLNKKLEGGQQVGMHSKGTQTAKEEMEMDPKPDLDSDSWCLLGTDSCRPSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TD19 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
C11 S-Nitrosylation
S13 Phosphorylation
S16 Phosphorylation
S20 Phosphorylation
S22 Phosphorylation
C25 S-Nitrosylation
S28 Phosphorylation
S29 Phosphorylation Q8TD19 (NEK9)
S35 Phosphorylation
Y52 Phosphorylation
R60 Methylation
T72 Phosphorylation
S76 Phosphorylation
K81 Ubiquitination
K191 Ubiquitination
K199 Ubiquitination
K200 Ubiquitination
S206 Phosphorylation
T210 Phosphorylation Q8TD19 (NEK9)
T214 Phosphorylation
T254 Phosphorylation
K262 Ubiquitination
K318 Ubiquitination
T326 Phosphorylation
K327 Ubiquitination
S331 Phosphorylation
S332 Phosphorylation Q8TD19 (NEK9)
T333 Phosphorylation Q8TD19 (NEK9)
T335 Phosphorylation Q8TD19 (NEK9)
Y350 Phosphorylation
K356 Ubiquitination
S357 Phosphorylation
T358 Phosphorylation Q8TD19 (NEK9)
K361 Ubiquitination
K366 Ubiquitination
K411 Ubiquitination
S413 Phosphorylation
K422 Ubiquitination
K499 Ubiquitination
Y509 Phosphorylation
S516 Phosphorylation
Y520 Phosphorylation
K525 Ubiquitination
K563 Ubiquitination
K606 Ubiquitination
K625 Ubiquitination
K635 Ubiquitination
K650 Ubiquitination
T685 Phosphorylation
K731 Acetylation
K731 Ubiquitination
T732 Phosphorylation
S735 Phosphorylation Q8TD19 (NEK9)
S741 Phosphorylation
S748 Phosphorylation
S749 Phosphorylation
S750 Phosphorylation
S765 Phosphorylation
S769 Phosphorylation
S793 Phosphorylation
T795 Phosphorylation
S801 Phosphorylation
S805 Phosphorylation
S827 Phosphorylation
S829 Phosphorylation
S832 Phosphorylation
S836 Phosphorylation
S838 Phosphorylation
T842 Phosphorylation
Y845 Phosphorylation
K852 Ubiquitination
S855 Phosphorylation
K862 Ubiquitination
S868 Phosphorylation Q8TD19 (NEK9)
S869 Phosphorylation
T877 Phosphorylation
T883 Phosphorylation
T886 Phosphorylation Q8TD19 (NEK9)
K929 Ubiquitination
K933 Ubiquitination
S944 Phosphorylation Q8TD19 (NEK9)
K945 Ubiquitination
S964 Phosphorylation
S974 Phosphorylation
S978 Phosphorylation

PTMs - Q8TD19 As Enzyme

Substrate Site Source
Q8NHV4 (NEDD1) S377 Uniprot
Q8TD19 (NEK9) S29 Uniprot
Q8TD19 (NEK9) T210 Uniprot
Q8TD19 (NEK9) S332 Uniprot
Q8TD19 (NEK9) T333 Uniprot
Q8TD19 (NEK9) T335 Uniprot
Q8TD19 (NEK9) T358 Uniprot
Q8TD19 (NEK9) S735 Uniprot
Q8TD19 (NEK9) S868 Uniprot
Q8TD19 (NEK9) T886 Uniprot
Q8TD19 (NEK9) S944 Uniprot
Q8TDX7-1 (NEK7) S195 Uniprot
Q9HC98-1 (NEK6) S206 Uniprot

Research Backgrounds

Function:

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.

PTMs:

Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

Subunit Structure:

Homodimer. Binds to Ran GTPase. Has a greater affinity for Ran-GDP over Ran-GTP. Interacts with NEK6, NEK7 and BICD2. Interacts with SSRP1 and SUPT16H, the 2 subunits of the FACT complex. Interacts with DYNLL1; phosphorylation at Ser-944 strongly reduces DYNLL1 binding.

Family&Domains:

Dimerizes through its coiled-coil domain.

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

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