NFATc2IP Antibody - #AF9128

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Product: NFATc2IP Antibody
Catalog: AF9128
Description: Rabbit polyclonal antibody to NFATc2IP
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 48kDa; 46kD(Calculated).
Uniprot: Q8NCF5
RRID: AB_2843318

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
NFATc2IP Antibody detects endogenous levels of total NFATc2IP.
RRID:
AB_2843318
Cite Format: Affinity Biosciences Cat# AF9128, RRID:AB_2843318.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

45 kDa NF-AT-interacting protein; 45 kDa NFAT-interacting protein; cytoplasmic 2-interacting protein; ESC2; NF2IP_HUMAN; NFAT-interacting protein, 45-KD; NFATC2-interacting protein; Nfatc2ip; NIP45; Nuclear factor of activated T-cells; Nuclear factor of activated T-cells, cytoplasmic 2-interacting protein; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein; RAD60;

Immunogens

Immunogen:
Uniprot:

Q8NCF5(NF2IP_HUMAN) >>Visit HPA database.

Gene(ID):
NFATC2IP(84901)
Sequence:
MAEPVGKRGRWSGGSGAGRGGRGGWGGRGRRPRAQRSPSRGTLDVVSVDLVTDSDEEILEVATARGAADEVEVEPPEPPGPVASRDNSNSDSEGEDRRPAGPPREPVRRRRRLVLDPGEAPLVPVYSGKVKSSLRLIPDDLSLLKLYPPGDEEEAELADSSGLYHEGSPSPGSPWKTKLRTKDKEEKKKTEFLDLDNSPLSPPSPRTKSRTHTRALKKLSEVNKRLQDLRSCLSPKPPQGQEQQGQEDEVVLVEGPTLPETPRLFPLKIRCRADLVRLPLRMSEPLQSVVDHMATHLGVSPSRILLLFGETELSPTATPRTLKLGVADIIDCVVLTSSPEATETSQQLQLRVQGKEKHQTLEVSLSRDSPLKTLMSHYEEAMGLSGRKLSFFFDGTKLSGRELPADLGMESGDLIEVWG

PTMs - Q8NCF5 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
S37 Phosphorylation
T52 Phosphorylation
S54 Phosphorylation
S84 Phosphorylation
S88 Phosphorylation
S90 Phosphorylation
S92 Phosphorylation
Y126 Phosphorylation
S127 Phosphorylation
K129 Sumoylation
K129 Ubiquitination
K145 Ubiquitination
S168 Phosphorylation
S170 Phosphorylation
S173 Phosphorylation
K176 Ubiquitination
T190 Phosphorylation
S198 Phosphorylation
S201 Phosphorylation
S204 Phosphorylation
K208 Acetylation
K218 Methylation
K218 Sumoylation
S220 Phosphorylation
K224 Ubiquitination
S231 Phosphorylation
S234 Phosphorylation
T261 Phosphorylation
K268 Ubiquitination
S300 Phosphorylation
T311 Phosphorylation
S314 Phosphorylation
T316 Phosphorylation
T318 Phosphorylation
S338 Phosphorylation
T342 Phosphorylation
K357 Sumoylation
S364 Phosphorylation
S366 Phosphorylation
S369 Phosphorylation
T373 Phosphorylation
S385 Phosphorylation
K388 Sumoylation
K388 Ubiquitination
S390 Phosphorylation
K397 Sumoylation

Research Backgrounds

Function:

In T-helper 2 (Th2) cells, regulates the magnitude of NFAT-driven transcription of a specific subset of cytokine genes, including IL3, IL4, IL5 and IL13, but not IL2. Recruits PRMT1 to the IL4 promoter; this leads to enhancement of histone H4 'Arg-3'-methylation and facilitates subsequent histone acetylation at the IL4 locus, thus promotes robust cytokine expression (By similarity). Down-regulates formation of poly-SUMO chains by UBE2I/UBC9 (By similarity).

PTMs:

Methylation at the N-terminus by PRMT1 modulates interaction with the NFAT complex and results in augmented cytokine production.

Subcellular Location:

Nucleus. Cytoplasm.
Note: TRAF1 is associated with a fraction of NFATC2IP in the cytoplasm and prevents its translocation to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with NFATC2, TRAF1, TRAF2 and PRMT1. Interacts with UBE2I/UBC9 (By similarity).

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