NFRKB Antibody - #AF9129
(1)
| Product: | NFRKB Antibody |
| Catalog: | AF9129 |
| Description: | Rabbit polyclonal antibody to NFRKB |
| Application: | WB IF/ICC |
| Reactivity: | Human, Rat, Monkey |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 110,180kD; 139kD(Calculated). |
| Uniprot: | Q6P4R8 |
| RRID: | AB_2843319 |
Product Info
Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat,Monkey
Prediction:
Pig(86%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
NFRKB Antibody detects endogenous levels of total NFRKB.
RRID:
AB_2843319
Cite Format: Affinity Biosciences Cat# AF9129, RRID:AB_2843319.
Cite Format: Affinity Biosciences Cat# AF9129, RRID:AB_2843319.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
A530090G11Rik; DKFZp547B2013; DNA binding protein R kappa B; INO80 complex subunit G; INO80G; Nuclear factor related to kappa B binding protein; Nuclear factor related to kappaB binding protein;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q6P4R8 NFRKB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDSLDHMLTDPLELGPCGDGHGTRIMEDCLLGGTRVSLPEDLLEDPEIFFDVVSLSTWQEVLSDSQREHLQQFLPQFPEDSAEQQNELILALFSGENFRFGNPLHIAQKLFRDGHFNPEVVKYRQLCFKSQYKRYLNSQQQYFHRLLKQILASRSDLLEMARRSGPALPFRQKRPSPSRTPEEREWRTQQRYLKVLREVKEECGDTALSSDEEDLSSWLPSSPARSPSPAVPLRVVPTLSTTDMKTADKVELGDSDLKIMLKKHHEKRKHQPDHPDLLTGDLTLNDIMTRVNAGRKGSLAALYDLAVLKKKVKEKEEKKKKKIKTIKSEAEDLAEPLSSTEGVAPLSQAPSPLAIPAIKEEPLEDLKPCLGINEISSSFFSLLLEILLLESQASLPMLEERVLDWQSSPASSLNSWFSAAPNWAELVLPALQYLAGESRAVPSSFSPFVEFKEKTQQWKLLGQSQDNEKELAALFQLWLETKDQAFCKQENEDSSDATTPVPRVRTDYVVRPSTGEEKRVFQEQERYRYSQPHKAFTFRMHGFESVVGPVKGVFDKETSLNKAREHSLLRSDRPAYVTILSLVRDAAARLPNGEGTRAEICELLKDSQFLAPDVTSTQVNTVVSGALDRLHYEKDPCVKYDIGRKLWIYLHRDRSEEEFERIHQAQAAAAKARKALQQKPKPPSKVKSSSKESSIKVLSSGPSEQSQMSLSDSSMPPTPVTPVTPTTPALPAIPISPPPVSAVNKSGPSTVSEPAKSSSGVLLVSSPTMPHLGTMLSPASSQTAPSSQAAARVVSHSGSAGLSQVRVVAQPSLPAVPQQSGGPAQTLPQMPAGPQIRVPATATQTKVVPQTVMATVPVKAQTTAATVQRPGPGQTGLTVTSLPATASPVSKPATSSPGTSAPSASTAAVIQNVTGQNIIKQVAITGQLGVKPQTGNSIPLTATNFRIQGKDVLRLPPSSITTDAKGQTVLRITPDMMATLAKSQVTTVKLTQDLFGTGGNTTGKGISATLHVTSNPVHAADSPAKASSASAPSSTPTGTTVVKVTPDLKPTEASSSAFRLMPALGVSVADQKGKSTVASSEAKPAATIRIVQGLGVMPPKAGQTITVATHAKQGASVASGSGTVHTSAVSLPSMNAAVSKTVAVASGAASTPISISTGAPTVRQVPVSTTVVSTSQAGKLPTRITVPLSVISQPMKGKSVVTAPIIKGNLGANLSGLGRNIILTTMPAGTKLIAGNKPVSFLTAQQLQQLQQQGQATQVRIQTVPASHLQQGTASGSSKAVSTVVVTTAPSPKQAPEQQ
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
88
Sheep
88
Pig
86
Chicken
86
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6P4R8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K109 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K148 | Acetylation | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| T180 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| K459 | Ubiquitination | Uniprot | |
| K488 | Sumoylation | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| Y576 | Phosphorylation | Uniprot | |
| T578 | Phosphorylation | Uniprot | |
| K634 | Ubiquitination | Uniprot | |
| S655 | Phosphorylation | Uniprot | |
| T750 | O-Glycosylation | Uniprot | |
| S766 | Phosphorylation | Uniprot | |
| T768 | Phosphorylation | Uniprot | |
| T774 | Phosphorylation | Uniprot | |
| S777 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| S781 | Phosphorylation | Uniprot | |
| S799 | Phosphorylation | Uniprot | |
| S803 | Phosphorylation | Uniprot | |
| T841 | O-Glycosylation | Uniprot | |
| S887 | Phosphorylation | Uniprot | |
| K931 | Ubiquitination | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| K965 | Ubiquitination | Uniprot | |
| K982 | Ubiquitination | Uniprot | |
| K989 | Ubiquitination | Uniprot | |
| K1004 | Ubiquitination | Uniprot | |
| S1022 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| T1035 | Phosphorylation | Uniprot | |
| T1037 | Phosphorylation | Uniprot | |
| T1039 | Phosphorylation | Uniprot | |
| K1049 | Acetylation | Uniprot | |
| K1049 | Ubiquitination | Uniprot | |
| T1076 | Phosphorylation | Uniprot | |
| K1083 | Acetylation | Uniprot | |
| K1083 | Sumoylation | Uniprot | |
| K1083 | Ubiquitination | Uniprot | |
| T1087 | Phosphorylation | Uniprot | |
| S1192 | Phosphorylation | Uniprot | |
| K1196 | Methylation | Uniprot | |
| K1198 | Methylation | Uniprot | |
| K1198 | Ubiquitination | Uniprot | |
| S1199 | Phosphorylation | Uniprot | |
| T1202 | Phosphorylation | Uniprot | |
| K1207 | Ubiquitination | Uniprot | |
| T1230 | Phosphorylation | Uniprot | |
| K1237 | Acetylation | Uniprot | |
| K1237 | Ubiquitination | Uniprot | |
| T1273 | O-Glycosylation | Uniprot | |
| S1291 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'.
Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Modulates the deubiquitinase activity of UCHL5 in the INO80 complex.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in thymus, brain, testes, spleen and liver.
Subunit Structure:
Component of the chromatin remodeling INO80 complex; specifically part of a complex module associated with the N-terminus of INO80. Interacts with UCHL5; NFRKB competes with ADRM1 for interaction with UCHL5.
Family&Domains:
NFRKB seems to be mostly disordered. The wing-helix like domain doesn't bind DNA.
Belongs to the NFRKB family.
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