Nicalin Antibody - #AF9130
| Product: | Nicalin Antibody |
| Catalog: | AF9130 |
| Description: | Rabbit polyclonal antibody to Nicalin |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Dog, Xenopus |
| Mol.Wt.: | 63kDa, 70 kDa; 63kD(Calculated). |
| Uniprot: | Q969V3 |
| RRID: | AB_2843320 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9130, RRID:AB_2843320.
Fold/Unfold
NCLN; NCLN_HUMAN; NET59; Nicalin; Nicalin homolog (zebrafish); Nicastrin like protein; Nicastrin-like protein;
Immunogens
- Q969V3 NCLN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLEEAGEVLENMLKASCLPLGFIVFLPAVLLLVAPPLPAADAAHEFTVYRMQQYDLQGQPYGTRNAVLNTEARTMAAEVLSRRCVLMRLLDFSYEQYQKALRQSAGAVVIILPRAMAAVPQDVVRQFMEIEPEMLAMETAVPVYFAVEDEALLSIYKQTQAASASQGSASAAEVLLRTATANGFQMVTSGVQSKAVSDWLIASVEGRLTGLGGEDLPTIVIVAHYDAFGVAPWLSLGADSNGSGVSVLLELARLFSRLYTYKRTHAAYNLLFFASGGGKFNYQGTKRWLEDNLDHTDSSLLQDNVAFVLCLDTVGRGSSLHLHVSKPPREGTLQHAFLRELETVAAHQFPEVRFSMVHKRINLAEDVLAWEHERFAIRRLPAFTLSHLESHRDGQRSSIMDVRSRVDSKTLTRNTRIIAEALTRVIYNLTEKGTPPDMPVFTEQMQIQQEQLDSVMDWLTNQPRAAQLVDKDSTFLSTLEHHLSRYLKDVKQHHVKADKRDPEFVFYDQLKQVMNAYRVKPAVFDLLLAVGIAAYLGMAYVAVQHFSLLYKTVQRLLVKAKTQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q969V3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K99 | Ubiquitination | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| K286 | Ubiquitination | Uniprot | |
| K326 | Ubiquitination | Uniprot | |
| K359 | Ubiquitination | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| T423 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| K511 | Ubiquitination | Uniprot |
Research Backgrounds
May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning.
Endoplasmic reticulum membrane>Single-pass membrane protein.
Highly expressed in pancreas and skeletal muscle and, at lower levels, in heart.
Forms a complex with NOMO2 and TMEM147, resulting in a stabilization of the 3 proteins, which are otherwise quickly degraded by the proteasome. Due to the strong similarity between NOMO1, NOMO2 and NOMO3, probably also interacts with NOMO1 and NOMO3. Participates in a large protein complex, which is not related to the gamma-secretase complex.
Belongs to the nicastrin family.
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