Nidogen Antibody - #AF9131
(3)
| Product: | Nidogen Antibody |
| Catalog: | AF9131 |
| Description: | Rabbit polyclonal antibody to Nidogen |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 136kDa; 136kD(Calculated). |
| Uniprot: | P14543 |
| RRID: | AB_2843321 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
Nidogen Antibody detects endogenous levels of total Nidogen.
RRID:
AB_2843321
Cite Format: Affinity Biosciences Cat# AF9131, RRID:AB_2843321.
Cite Format: Affinity Biosciences Cat# AF9131, RRID:AB_2843321.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Enactin; Ent; Entactin 1; Entactin; NID 1; NID; NID-1; Nid1; NID1_HUMAN; Nidogen (enactin); Nidogen 1; Nidogen; Nidogen-1;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P14543 NID1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLASSSRIRAAWTRALLLPLLLAGPVGCLSRQELFPFGPGQGDLELEDGDDFVSPALELSGALRFYDRSDIDAVYVTTNGIIATSEPPAKESHPGLFPPTFGAVAPFLADLDTTDGLGKVYYREDLSPSITQRAAECVHRGFPEISFQPSSAVVVTWESVAPYQGPSRDPDQKGKRNTFQAVLASSDSSSYAIFLYPEDGLQFHTTFSKKENNQVPAVVAFSQGSVGFLWKSNGAYNIFANDRESVENLAKSSNSGQQGVWVFEIGSPATTNGVVPADVILGTEDGAEYDDEDEDYDLATTRLGLEDVGTTPFSYKALRRGGADTYSVPSVLSPRRAATERPLGPPTERTRSFQLAVETFHQQHPQVIDVDEVEETGVVFSYNTDSRQTCANNRHQCSVHAECRDYATGFCCSCVAGYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVPIVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPVGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFVGHPGNLVIKQRFSGIDEHGHLTIDTELEGRVPQIPFGSSVHIEPYTELYHYSTSVITSSSTREYTVTEPERDGASPSRIYTYQWRQTITFQECVHDDSRPALPSTQQLSVDSVFVLYNQEEKILRYALSNSIGPVREGSPDALQNPCYIGTHGCDTNAACRPGPRTQFTCECSIGFRGDGRTCYDIDECSEQPSVCGSHTICNNHPGTFRCECVEGYQFSDEGTCVAVVDQRPINYCETGLHNCDIPQRAQCIYTGGSSYTCSCLPGFSGDGQACQDVDECQPSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCVPGEVEKTRCQHEREHILGAAGATDPQRPIPPGLFVPECDAHGHYAPTQCHGSTGYCWCVDRDGREVEGTRTRPGMTPPCLSTVAPPIHQGPAVPTAVIPLPPGTHLLFAQTGKIERLPLEGNTMRKTEAKAFLHVPAKVIIGLAFDCVDKMVYWTDITEPSIGRASLHGGEPTTIIRQDLGSPEGIAVDHLGRNIFWTDSNLDRIEVAKLDGTQRRVLFETDLVNPRGIVTDSVRGNLYWTDWNRDNPKIETSYMDGTNRRILVQDDLGLPNGLTFDAFSSQLCWVDAGTNRAECLNPSQPSRRKALEGLQYPFAVTSYGKNLYFTDWKMNSVVALDLAISKETDAFQPHKQTRLYGITTALSQCPQGHNYCSVNNGGCTHLCLATPGSRTCRCPDNTLGVDCIEQK
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
92
Xenopus
75
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P14543 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T100 | Phosphorylation | Uniprot | |
| T113 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| T310 | O-Glycosylation | Uniprot | |
| T311 | O-Glycosylation | Uniprot | |
| R320 | Methylation | Uniprot | |
| Y326 | Phosphorylation | Uniprot | |
| S327 | O-Glycosylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S330 | O-Glycosylation | Uniprot | |
| S333 | O-Glycosylation | Uniprot | |
| S333 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T339 | O-Glycosylation | Uniprot | |
| T347 | O-Glycosylation | Uniprot | |
| T350 | O-Glycosylation | Uniprot | |
| R423 | Methylation | Uniprot | |
| T517 | Phosphorylation | Uniprot | |
| Y832 | Phosphorylation | Uniprot | |
| T909 | Phosphorylation | Uniprot | |
| T922 | O-Glycosylation | Uniprot | |
| T935 | O-Glycosylation | Uniprot | |
| T1053 | Phosphorylation | Uniprot | |
| T1098 | Phosphorylation | Uniprot | |
| S1181 | Phosphorylation | Uniprot | |
| T1184 | Phosphorylation | Uniprot | |
| T1193 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
PTMs:
N- and O-glycosylated.
Subcellular Location:
Secreted>Extracellular space>Extracellular matrix>Basement membrane.
Subunit Structure:
Interacts with FBLN1 and LGALS3BP (By similarity). Interacts with PLXDC1.
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