Nox3 Antibody - #AF9134

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Product: Nox3 Antibody
Catalog: AF9134
Description: Rabbit polyclonal antibody to Nox3
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 65kDa; 65kD(Calculated).
Uniprot: Q9HBY0
RRID: AB_2843324

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(80%), Bovine(80%), Horse(89%), Sheep(80%), Rabbit(80%), Dog(89%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
Nox3 Antibody detects endogenous levels of total Nox3.
RRID:
AB_2843324
Cite Format: Affinity Biosciences Cat# AF9134, RRID:AB_2843324.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 1.6.3.-; GP91 3; GP91-3; gp91phox homolog 3; Mitogenic oxidase 2; MOX 2; MOX-2; MOX2; NADPH oxidase 3; NADPH oxidase catalytic subunit-like 3; NOX 3; Nox3; NOX3_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9HBY0(NOX3_HUMAN) >>Visit HPA database.

Gene(ID):
NOX3(50508)
Sequence:
MMGCWILNEGLSTILVLSWLGINFYLFIDTFYWYEEEESFHYTRVILGSTLAWARASALCLNFNCMLILIPVSRNLISFIRGTSICCRGPWRRQLDKNLRFHKLVAYGIAVNATIHIVAHFFNLERYHWSQSEEAQGLLAALSKLGNTPNESYLNPVRTFPTNTTTELLRTIAGVTGLVISLALVLIMTSSTEFIRQASYELFWYTHHVFIVFFLSLAIHGTGRIVRGQTQDSLSLHNITFCRDRYAEWQTVAQCPVPQFSGKEPSAWKWILGPVVLYACERIIRFWRFQQEVVITKVVSHPSGVLELHMKKRGFKMAPGQYILVQCPAISSLEWHPFTLTSAPQEDFFSVHIRAAGDWTAALLEAFGAEGQALQEPWSLPRLAVDGPFGTALTDVFHYPVCVCVAAGIGVTPFAALLKSIWYKCSEAQTPLKLSKVYFYWICRDARAFEWFADLLLSLETRMSEQGKTHFLSYHIFLTGWDENQALHIALHWDENTDVITGLKQKTFYGRPNWNNEFKQIAYNHPSSSIGVFFCGPKALSRTLQKMCHLYSSADPRGVHFYYNKESF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
89
Dog
89
Chicken
89
Pig
80
Bovine
80
Sheep
80
Rabbit
80
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBY0 As Substrate

Site PTM Type Enzyme
K311 Acetylation
K312 Acetylation

Research Backgrounds

Function:

NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with and stabilizes CYBA/p22phox.

References

1). Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway. Andrology, 2020 (PubMed: 32012485) [IF=3.2]

Application: WB    Species: rat    Sample: testis

FIGURE 3|BB attenuates ROS production in the testis of DM rats. (A) Representative Western blot results for NOX5, p22phox, gp91phox, NOX1, NOX3, and NOX4 in the testes of rats from all four groups.
2). Synergistic effect of elevated glucose levels with SARS-CoV-2 spike protein induced NOX-dependent ROS production in endothelial cells. Molecular Biology Reports, 2023 (PubMed: 37289363) [IF=2.6]

Application: WB    Species: Human    Sample: HUVECs

Fig. 1 Protein expression in different doses of S protein induced HUVECs. A. ACE2 and TMPRSS2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. B. Relative expression of gray values for ACE2. C. Relative expression of gray values for TMPRSS2. D. NOX1 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. E. Relative expression of gray values for NOX1. F. NOX2 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. G. Relative expression of gray values for NOX2. H. NOX3 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. I. Relative expression of gray values for NOX3. J. NOX4 protein expression relative to GAPDH analyzed by western blot in different doses of S protein induced HUVECs. K. Relative expression of gray values for NOX4

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