OTUB2 Antibody - #AF9147

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Product: OTUB2 Antibody
Catalog: AF9147
Description: Rabbit polyclonal antibody to OTUB2
Application: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 27kDa; 27kD(Calculated).
Uniprot: Q96DC9
RRID: AB_2843337

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%)
Clonality:
Polyclonal
Specificity:
OTUB2 Antibody detects endogenous levels of total OTUB2.
RRID:
AB_2843337
Cite Format: Affinity Biosciences Cat# AF9147, RRID:AB_2843337.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C14orf137; Deubiquitinating enzyme OTUB2; FLJ21916; MGC3102; OTB2; OTU domain containing ubiquitin aldehyde binding protein 2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; OTU2; Otub2; OTUB2_HUMAN; Otubain 2; Otubain-2; Ubiquitin specific processing protease OTUB2; Ubiquitin specific protease otubain 2; Ubiquitin thioesterase OTUB2; Ubiquitin-specific-processing protease OTUB2;

Immunogens

Immunogen:

A synthesized peptide derived from human OTUB2, corresponding to a region within the internal amino acids.

Uniprot:

Q96DC9(OTUB2_HUMAN) >>Visit HPA database.

Gene(ID):
OTUB2(78990)
Expression:
Q96DC9 OTUB2_HUMAN:

Widely expressed. Expressed at higher level in brain.

Sequence:
MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
89
Rabbit
89
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.

Tissue Specificity:

Widely expressed. Expressed at higher level in brain.

Family&Domains:

Belongs to the peptidase C65 family.

References

1). Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1. Nature communications, 2024 (PubMed: 38167274) [IF=16.6]
2). OTUB2 Facilitates Tumorigenesis of Gastric Cancer Through Promoting KDM1A-Mediated Stem Cell-Like Properties. Frontiers in Oncology, 2023 (PubMed: 34646768) [IF=3.5]

Application: WB    Species: Human    Sample: GC cells

Figure 2 Otubain 2 (OTUB2) promotes the maintenance of gastric cancer (GC) stem cell properties. (A) Relative protein expression levels of OTUB2 in GC cells (MKN-45, SGC-7901, SNU-1, HGC-27, and AGS) and gastric epithelial cells-1 (GES-1) were detected by Western blot. Actin served as internal control. (B) The protein levels of OTUB2, Nanog, ALDH, and Oct4 in GC cells were measured by Western blot after transfected with OTUB2 overexpression vector (MKN-45 and SGC-7901 cells) or shRNA targeting OTUB2 (HGC-27 and AGS cells). Actin served as internal control. (C) The effects of OTUB2 overexpression (MKN-45 and SGC-7901 cells) or knockdown (HGC-27 and AGS cells) on spheroid-forming ability were detected by sphere formation assay. (D) After cells were transfected with OTUB2 overexpression vector (MKN-45 and SGC-7901 cells) or shRNA targeting OTUB2 (HGC-27 and AGS cells), percentage of CD44 and CD133 double positive cells was detected by flow cytometry. Data was represented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.

Application: IF/ICC    Species: Human    Sample: GC cells

Figure 3 Otubain 2 (OTUB2) promotes the proliferation, invasion, and migration of gastric cancer (GC) cells. (A) Cell viability was detected in OTUB2-overexpressed (MKN-45 and SGC-7901 cells) or downregulated GC cells (HGC-27 and AGS cells). (B) Proliferating OTUB2-overexpressed (MKN-45 and SGC-7901 cells) or downregulated GC cells (HGC-27 and AGS cells) were labeled with EdU (red); cell nuclei were stained with DAPI (blue). Wound healing assays (C) and Transwell assays (D) were used to determine the changes in migratory and invasive abilities of GC cells transfected with OTUB2 overexpression vector (MKN-45 and SGC-7901 cells) or shRNA targeting OTUB2 (HGC-27 and AGS cells). Data was represented as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.

Application: IHC    Species: Human    Sample: GC cells

Figure 4 Otubain 2 (OTUB2) promotes xenograft tumor growth in vivo. (A) The nude mice were injected with GC cells, which were transfected with OTUB2 overexpression vector (SGC-7901 cells) or sh-OTUB2 (HGC-27 cells), respectively. Tumor volumes were calculated after injection every 5 days, tumor weight was measured 35 days after injection. (B) The tumor sections were underimmumohistochemical staining using antibodies against OTUB2 or Ki-67. Data was represented as mean ± SD. **P < 0.01.

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