PHF3 Antibody - #AF9154

(2)  
Product: PHF3 Antibody
Catalog: AF9154
Description: Rabbit polyclonal antibody to PHF3
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 230kDa; 229kD(Calculated).
Uniprot: Q92576
RRID: AB_2843344

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PHF3 Antibody detects endogenous levels of total PHF3.
RRID:
AB_2843344
Cite Format: Affinity Biosciences Cat# AF9154, RRID:AB_2843344.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PHD finger protein 3; PHF3; PHF3_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q92576(PHF3_HUMAN) >>Visit HPA database.

Gene(ID):
PHF3(23469)
Expression:
Q92576 PHF3_HUMAN:

Ubiquitous. Expression is significantly reduced or lost in glioblastomas, glioblastoma cell lines, anaplastic astrocytomas, and astrocytomas.

Sequence:
MDIVDTFNHLIPTEHLDDALFLGSNLENEVCEDFSASQNVLEDSLKNMLSDKDPMLGSASNQFCLPVLDSNDPNFQMPCSTVVGLDDIMDEGVVKESGNDTIDEEELILPNRNLRDKVEENSVRSPRKSPRLMAQEQVRSLRQSTIAKRSNAAPLSNTKKASGKTVSTAKAGVKQPERSQVKEEVCMSLKPEYHKENRRCSRNSGQIEVVPEVSVSSSHSSVSSCLEMKDEDGLDSKHKCNNPGEIDVPSHELNCSLLSETCVTIGEKKNEALMECKAKPVGSPLFKFSDKEEHEQNDSISGKTGETVVEEMIATRKVEQDSKETVKLSHEDDHILEDAGSSDISSDAACTNPNKTENSLVGLPSCVDEVTECNLELKDTMGIADKTENTLERNKIEPLGYCEDAESNRQLESTEFNKSNLEVVDTSTFGPESNILENAICDVPDQNSKQLNAIESTKIESHETANLQDDRNSQSSSVSYLESKSVKSKHTKPVIHSKQNMTTDAPKKIVAAKYEVIHSKTKVNVKSVKRNTDVPESQQNFHRPVKVRKKQIDKEPKIQSCNSGVKSVKNQAHSVLKKTLQDQTLVQIFKPLTHSLSDKSHAHPGCLKEPHHPAQTGHVSHSSQKQCHKPQQQAPAMKTNSHVKEELEHPGVEHFKEEDKLKLKKPEKNLQPRQRRSSKSFSLDEPPLFIPDNIATIRREGSDHSSSFESKYMWTPSKQCGFCKKPHGNRFMVGCGRCDDWFHGDCVGLSLSQAQQMGEEDKEYVCVKCCAEEDKKTEILDPDTLENQATVEFHSGDKTMECEKLGLSKHTTNDRTKYIDDTVKHKVKILKRESGEGRNSSDCRDNEIKKWQLAPLRKMGQPVLPRRSSEEKSEKIPKESTTVTCTGEKASKPGTHEKQEMKKKKVEKGVLNVHPAASASKPSADQIRQSVRHSLKDILMKRLTDSNLKVPEEKAAKVATKIEKELFSFFRDTDAKYKNKYRSLMFNLKDPKNNILFKKVLKGEVTPDHLIRMSPEELASKELAAWRRRENRHTIEMIEKEQREVERRPITKITHKGEIEIESDAPMKEQEAAMEIQEPAANKSLEKPEGSEKQKEEVDSMSKDTTSQHRQHLFDLNCKICIGRMAPPVDDLSPKKVKVVVGVARKHSDNEAESIADALSSTSNILASEFFEEEKQESPKSTFSPAPRPEMPGTVEVESTFLARLNFIWKGFINMPSVAKFVTKAYPVSGSPEYLTEDLPDSIQVGGRISPQTVWDYVEKIKASGTKEICVVRFTPVTEEDQISYTLLFAYFSSRKRYGVAANNMKQVKDMYLIPLGATDKIPHPLVPFDGPGLELHRPNLLLGLIIRQKLKRQHSACASTSHIAETPESAPPIALPPDKKSKIEVSTEEAPEEENDFFNSFTTVLHKQRNKPQQNLQEDLPTAVEPLMEVTKQEPPKPLRFLPGVLIGWENQPTTLELANKPLPVDDILQSLLGTTGQVYDQAQSVMEQNTVKEIPFLNEQTNSKIEKTDNVEVTDGENKEIKVKVDNISESTDKSAEIETSVVGSSSISAGSLTSLSLRGKPPDVSTEAFLTNLSIQSKQEETVESKEKTLKRQLQEDQENNLQDNQTSNSSPCRSNVGKGNIDGNVSCSENLVANTARSPQFINLKRDPRQAAGRSQPVTTSESKDGDSCRNGEKHMLPGLSHNKEHLTEQINVEEKLCSAEKNSCVQQSDNLKVAQNSPSVENIQTSQAEQAKPLQEDILMQNIETVHPFRRGSAVATSHFEVGNTCPSEFPSKSITFTSRSTSPRTSTNFSPMRPQQPNLQHLKSSPPGFPFPGPPNFPPQSMFGFPPHLPPPLLPPPGFGFAQNPMVPWPPVVHLPGQPQRMMGPLSQASRYIGPQNFYQVKDIRRPERRHSDPWGRQDQQQLDRPFNRGKGDRQRFYSDSHHLKRERHEKEWEQESERHRRRDRSQDKDRDRKSREEGHKDKERARLSHGDRGTDGKASRDSRNVDKKPDKPKSEDYEKDKEREKSKHREGEKDRDRYHKDRDHTDRTKSKR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
67
Xenopus
44
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92576 As Substrate

Site PTM Type Enzyme
S97 Phosphorylation
T101 Phosphorylation
S122 Phosphorylation
S125 Phosphorylation
S156 Phosphorylation
K159 Methylation
T168 Phosphorylation
S204 Phosphorylation
S256 Phosphorylation
S283 Phosphorylation
S289 Phosphorylation
S299 Phosphorylation
S342 Phosphorylation
S345 Phosphorylation
S359 Phosphorylation
T387 Phosphorylation
K395 Ubiquitination
R471 Methylation
S473 Phosphorylation
S476 Phosphorylation
K498 Acetylation
K513 Acetylation
Y514 Phosphorylation
K520 Ubiquitination
K566 Acetylation
T579 Phosphorylation
T584 Phosphorylation
K590 Acetylation
S677 Phosphorylation
S678 Phosphorylation
S680 Phosphorylation
S682 Phosphorylation
S702 Phosphorylation
T715 Phosphorylation
S752 Phosphorylation
Y818 Phosphorylation
T822 Phosphorylation
S834 Phosphorylation
S840 Phosphorylation
K858 Methylation
S868 Phosphorylation
S869 Phosphorylation
K872 Acetylation
K875 Acetylation
K878 Acetylation
K921 Acetylation
K961 Ubiquitination
K964 Sumoylation
K964 Ubiquitination
K989 Ubiquitination
T1006 Phosphorylation
S1014 Phosphorylation
K1021 Ubiquitination
T1034 Phosphorylation
T1054 Phosphorylation
S1063 Phosphorylation
S1084 Phosphorylation
S1133 Phosphorylation
S1148 Phosphorylation
S1154 Phosphorylation
S1163 Phosphorylation
S1168 Phosphorylation
S1178 Phosphorylation
S1181 Phosphorylation
T1182 Phosphorylation
S1184 Phosphorylation
T1194 Phosphorylation
Y1298 Phosphorylation
Y1312 Phosphorylation
T1319 Phosphorylation
S1356 Phosphorylation
S1401 Phosphorylation
T1516 Phosphorylation
S1588 Phosphorylation
S1613 Phosphorylation
S1614 Phosphorylation
S1618 Phosphorylation
S1630 Phosphorylation
T1639 Phosphorylation
S1642 Phosphorylation
K1649 Ubiquitination
S1703 Phosphorylation
S1708 Phosphorylation
S1722 Phosphorylation
S1724 Phosphorylation
S1731 Phosphorylation
R1756 Methylation
S1758 Phosphorylation
S1763 Phosphorylation
S1784 Phosphorylation
R1785 Methylation
R1790 Methylation
S1796 Phosphorylation
R1799 Methylation
K1809 Methylation
R1867 Methylation
R1877 Methylation
S1898 Phosphorylation
R1911 Methylation
R1915 Methylation
K1917 Methylation
R1922 Methylation
Y1924 Phosphorylation
S1925 Phosphorylation
S1927 Phosphorylation
S1952 Phosphorylation
S1975 Phosphorylation

Research Backgrounds

Tissue Specificity:

Ubiquitous. Expression is significantly reduced or lost in glioblastomas, glioblastoma cell lines, anaplastic astrocytomas, and astrocytomas.

Restrictive clause

 

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