Product: PRPF31 Antibody
Catalog: AF9169
Description: Rabbit polyclonal antibody to PRPF31
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Rabbit, Xenopus
Mol.Wt.: 55kDa; 55kD(Calculated).
Uniprot: Q8WWY3
RRID: AB_2843359

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Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PRPF31 Antibody detects endogenous levels of total PRPF31.
RRID:
AB_2843359
Cite Format: Affinity Biosciences Cat# AF9169, RRID:AB_2843359.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp566J153; hPrp 31; hPrp31; NY BR 99; Pre mRNA processing factor 31; Pre mRNA processing factor 31 homolog (yeast); Pre mRNA processing factor 31 homolog; Pre-mRNA-processing factor 31; Precursor mRNA-processing factor 31, S. cerevisiae, homolog of; Protein 61K; PRP 31; PRP31; PRP31 pre mRNA processing factor 31 homolog (yeast); PRP31 pre mRNA processing factor 31 homolog; PRP31_HUMAN; PRPF 31; prpf31; RP 11; RP11; Serologically defined breast cancer antigen NY BR 99; Serologically defined breast cancer antigen NY-BR-99; SNRNP61; U4/U6 small nuclear ribonucleoprotein Prp31; U4/U6 snRNP 61 kDa protein;

Immunogens

Immunogen:

A synthesized peptide derived from human PRPF31, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q8WWY3 PRP31_HUMAN:

Ubiquitously expressed.

Sequence:
MSLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSGDSVKTIAKLWDSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLVAAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSSMAEFLKVKGEKSGLMST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
36
Pig
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Involved in pre-mRNA splicing as component of the spliceosome. Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.

Subcellular Location:

Nucleus. Nucleus speckle. Nucleus>Cajal body.
Note: Predominantly found in speckles and in Cajal bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Family&Domains:

Interacts with the snRNP via the Nop domain.

The coiled coil domain is formed by two non-contiguous helices.

Belongs to the PRP31 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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