Rab 34 Antibody - #AF9174

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Product: Rab 34 Antibody
Catalog: AF9174
Description: Rabbit polyclonal antibody to Rab 34
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 29kDa; 29kD(Calculated).
Uniprot: Q9BZG1
RRID: AB_2843364

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
Rab 34 Antibody detects endogenous levels of total Rab 34.
RRID:
AB_2843364
Cite Format: Affinity Biosciences Cat# AF9174, RRID:AB_2843364.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Rab-39; RAB34; RAB34, member RAS oncogene family; RAB34_HUMAN; RAB39; RAH; Ras-related protein Rab-34; Ras-related protein Rab-39; Ras-related protein Rah;

Immunogens

Immunogen:
Uniprot:

Q9BZG1(RAB34_HUMAN) >>Visit HPA database.

Gene(ID):
RAB34(83871)
Sequence:
MNILAPVRRDRVLAELPQCLRKEAALHGHKDFHPRVTCACQEHRTGTVGFKISKVIVVGDLSVGKTCLINRFCKDTFDKNYKATIGVDFEMERFEVLGIPFSLQLWDTAGQERFKCIASTYYRGAQAIIIVFNLNDVASLEHTKQWLADALKENDPSSVLLFLVGSKKDLSTPAQYALMEKDALQVAQEMKAEYWAVSSLTGENVREFFFRVAALTFEANVLAELEKSGARRIGDVVRINSDDSNLYLTASKKKPTCCP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZG1 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K22 Ubiquitination
K30 Ubiquitination
K51 Ubiquitination
S62 Phosphorylation
K74 Ubiquitination
T76 Phosphorylation
K79 Ubiquitination
Y81 Phosphorylation
T108 Phosphorylation
K168 Ubiquitination
K181 Ubiquitination
S241 Phosphorylation
S244 Phosphorylation
Y247 Phosphorylation
K252 Ubiquitination

Research Backgrounds

Function:

Protein transport. Involved in the redistribution of lysosomes to the peri-Golgi region (By similarity). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Acts also as a positive regulator of hedgehog signaling and regulates ciliary function (By similarity).

Subcellular Location:

Cytoplasm. Golgi apparatus. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side. Cell projection>Cilium.
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with RILP.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

References

1). The Role of RAB GTPases and Its Potential in Predicting Immunotherapy Response and Prognosis in Colorectal Cancer. Frontiers in Genetics, 2022 (PubMed: 35154286) [IF=3.7]

Application: IHC    Species: Human    Sample: HCT16 cells and SW480 cells

FIGURE 8 Overexpression of RAB34 increased PD-L1 and PD-L2 expression of CRC. (A) Immunohistochemical staining of patients with colorectal cancer. (B) Overexpression of RAB34 analyzed the protein levels of PDL-1 and PD-L2 in the HCT16 cells and SW480 cells. (C) Overexpression of RAB34 analyzed the migration and invasion in the HCT16 cells and SW480 cells. * p < .05, ** p < .01, *** p < .001.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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