RHOBTB3 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

RHOBTB3 Antibody - Western blot analysis of RHOBTB3 using K562 whole cell lysates.

RHOBTB3 Antibody - AF9183 staining Hela cells by IF/ICC.

Product:	RHOBTB3 Antibody
Catalog:	AF9183
Description:	Rabbit polyclonal antibody to RHOBTB3
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	70kDa; 69kD(Calculated).
Uniprot:	O94955
RRID:	AB_2843373

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

RHOBTB3 Antibody detects endogenous levels of total RHOBTB3.

RRID:

AB_2843373
Cite Format: Affinity Biosciences Cat# AF9183, RRID:AB_2843373.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KIAA0878; Rho related BTB domain containing 3; Rho related BTB domain containing protein 3; RHOBTB 3;

Immunogens

Immunogen:

Uniprot:

O94955(RHBT3_HUMAN) >>Visit HPA database.

Gene(ID):

RHOBTB3(22836)

Expression:

O94955 RHBT3_HUMAN:

Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis.

Sequence:

O94955 RHBT3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSIHIVALGNEGDTFHQDNRPSGLIRTYLGRSPLVSGDESSLLLNAASTVARPVFTEYQASAFGNVKLVVHDCPVWDIFDSDWYTSRNLIGGADIIVIKYNVNDKFSFHEVKDNYIPVIKRALNSVPVIIAAVGTRQNEELPCTCPLCTSDRGSCVSTTEGIQLAKELGATYLELHSLDDFYIGKYFGGVLEYFMIQALNQKTSEKMKKRKMSNSFHGIRPPQLEQPEKMPVLKAEASHYNSDLNNLLFCCQCVDVVFYNPNLKKVVEAHKIVLCAVSHVFMLLFNVKSPTDIQDSSIIRTTQDLFAINRDTAFPGASHESSGNPPLRVIVKDALFCSCLSDILRFIYSGAFQWEELEEDIRKKLKDSGDVSNVIEKVKCILKTPGKINCLRNCKTYQARKPLWFYNTSLKFFLNKPMLADVVFEIQGTTVPAHRAILVARCEVMAAMFNGNYMEAKSVLIPVYGVSKETFLSFLEYLYTDSCCPAGIFQAMCLLICAEMYQVSRLQHICELFIITQLQSMPSRELASMNLDIVDLLKKAKFHHSDCLSTWLLHFIATNYLIFSQKPEFQDLSVEERSFVEKHRWPSNMYLKQLAEYRKYIHSRKCRCLVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Xenopus

100

Rabbit

100

Chicken

Bovine

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94955 As Substrate

O94955

Site	PTM Type	Source
K105	Ubiquitination	Uniprot
K112	Ubiquitination	Uniprot
K120	Ubiquitination	Uniprot
S125	Phosphorylation	Uniprot
T135	Phosphorylation	Uniprot
T144	Phosphorylation	Uniprot
T149	Phosphorylation	Uniprot
S150	Phosphorylation	Uniprot
K209	Acetylation	Uniprot
K211	Acetylation	Uniprot
K211	Ubiquitination	Uniprot
S215	Phosphorylation	Uniprot
K366	Ubiquitination	Uniprot
K377	Ubiquitination	Uniprot
K379	Acetylation	Uniprot
K383	Ubiquitination	Uniprot
K387	Ubiquitination	Uniprot
K401	Ubiquitination	Uniprot
S528	Phosphorylation	Uniprot
K538	Ubiquitination	Uniprot
K582	Ubiquitination	Uniprot
K592	Ubiquitination	Uniprot

Research Backgrounds

O94955_RHBT3_HUMAN

Function:

Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9.

Subcellular Location:

Golgi apparatus.

Tissue Specificity:

Ubiquitous. Highly expressed in neural and cardiac tissues, pancreas, placenta and testis.

Subunit Structure:

Interacts with RAB9A and RAB9B (at lower level compared to RAB9A-binding). Interacts with M6PRBP1/TIP47.

Family&Domains:

Although predicted to be a GTP-binding protein because of the presence of a Rho-like region, binds and hydrolyzes ATP. In contrast to Rho-like proteins, the conserved Asp residue in position 138 in the G4 region is replaced by an Asn, decreasing the ability to bind GTP.

References

1). MicroRNA-142-3p promotes renal cell carcinoma progression by targeting RhoBTB3 to regulate HIF-1 signaling and GGT/GSH pathways. Scientific reports, 2023 (PubMed: 37045834) [IF=3.8]

Application: WB Species: Human Sample: 786-O cells

Figure 4. miR-142-3p regulates RCC migration and invasion via hypoxia and GSH pathways by targeting RhoBTB3. 786-O cells were transfected with miR-control, miR-142-3p-mimics, and, miR-142-3p-inhibitors. Protein expression of RhoBTB3, hypoxia, and GSH pathway-related biomarkers, including HIF1A, VEGFA, and GGT1 using Western blot (A) (the original blots/gels are presented in Supplementary Fig. 3, the samples derive from the same experiment and that gels/blots were processed in parallel). The mRNA and protein expression of RhoBTB3, HIF1A, VEGFA, and GGT1 in 786-O cells was determined using qRT-PCR and Western blot, respectively (B,C). Expression of GGT1 differed significantly between RCC and the normal adjacent tissues (enhanced in KIRC, D, P

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

RHOBTB3 Antibody - #AF9183