SCAF1 Antibody - #AF9193

(2)  
Product: SCAF1 Antibody
Catalog: AF9193
Description: Rabbit polyclonal antibody to SCAF1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Rat, Pig, Bovine, Sheep, Dog
Mol.Wt.: 139kDa; 139kD(Calculated).
Uniprot: Q9H7N4
RRID: AB_2843383

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Rat(%), Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SCAF1 Antibody detects endogenous levels of total SCAF1.
RRID:
AB_2843383
Cite Format: Affinity Biosciences Cat# AF9193, RRID:AB_2843383.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:

Q9H7N4(SFR19_HUMAN) >>Visit HPA database.

Gene(ID):
SCAF1(58506)
Expression:
Q9H7N4 SFR19_HUMAN:

Ubiquitous. Highly expressed in fetal brain and liver, poorly expressed in salivary gland, heart, skin and ovary. Expressed in colorectal carcinomas and ovarian cancers. Overexpressed in colorectal carcinomas as compared to normal colonic mucosa.

Sequence:
MEEEDESRGKTEESGEDRGDGPPDRDPTLSPSAFILRAIQQAVGSSLQGDLPNDKDGSRCHGLRWRRCRSPRSEPRSQESGGTDTATVLDMATDSFLAGLVSVLDPPDTWVPSRLDLRPGESEDMLELVAEVRIGDRDPIPLPVPSLLPRLRAWRTGKTVSPQSNSSRPTCARHLTLGTGDGGPAPPPAPSSASSSPSPSPSSSSPSPPPPPPPPAPPAPPAPRFDIYDPFHPTDEAYSPPPAPEQKYDPFEPTGSNPSSSAGTPSPEEEEEEEEEEEEEEEDEEEEEGLSQSISRISETLAGIYDDNSLSQDFPGDESPRPDAQPTQPTPAPGTPPQVDSTRADGAMRRRVFVVGTEAEACREGKVSVEVVTAGGAALPPPLLPPGDSEIEEGEIVQPEEEPRLALSLFRPGGRAARPTPAASATPTAQPLPQPPAPRAPEGDDFLSLHAESDGEGALQVDLGEPAPAPPAADSRWGGLDLRRKILTQRRERYRQRSPSPAPAPAPAAAAGPPTRKKSRRERKRSGEAKEAASSSSGTQPAPPAPASPWDSKKHRSRDRKPGSHASSSARRRSRSRSRSRSTRRRSRSTDRRRGGSRRSRSREKRRRRRRSASPPPATSSSSSSRRERHRGKHRDGGGSKKKKKRSRSRGEKRSGDGSEKAPAPAPPPSGSTSCGDRDSRRRGAVPPSIQDLTDHDLFAIKRTITVGRLDKSDPRGPSPAPASSPKREVLYDSEGLSGEERGGKSSQKDRRRSGAASSSSSSREKGSRRKALDGGDRDRDRDRDRDRDRSSKKARPPKESAPSSGPPPKPPVSSGSGSSSSSSSCSSRKVKLQSKVAVLIREGVSSTTPAKDAASAGLGSIGVKFSRDRESRSPFLKPDERAPTEMAKAAPGSTKPKKTKVKAKAGAKKTKGTKGKTKPSKTRKKVRSGGGSGGSGGQVSLKKSKADSCSQAAGTKGAEETSWSGEERAAKVPSTPPPKAAPPPPALTPDSQTVDSSCKTPEVSFLPEEATEEAGVRGGAEEEEEEEEEEEEEEEEEEQQPATTTATSTAAAAPSTAPSAGSTAGDSGAEDGPASRVSQLPTLPPPMPWNLPAGVDCTTSGVLALTALLFKMEEANLASRAKAQELIQATNQILSHRKPPSSLGMTPAPVPTSLGLPPGPSSYLLPGSLPLGGCGSTPPTPTGLAATSDKREGSSSSEGRGDTDKYLKKLHTQERAVEEVKLAIKPYYQKKDITKEEYKDILRKAVHKICHSKSGEINPVKVSNLVRAYVQRYRYFRKHGRKPGDPPGPPRPPKEPGPPDKGGPGLPLPPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H7N4 As Substrate

Site PTM Type Enzyme
K10 Ubiquitination
S30 Phosphorylation
S32 Phosphorylation
K55 Ubiquitination
S146 Phosphorylation
K158 Ubiquitination
T159 Phosphorylation
S161 Phosphorylation
Y228 Phosphorylation
T234 Phosphorylation
Y238 Phosphorylation
S239 Phosphorylation
S309 Phosphorylation
S311 Phosphorylation
S319 Phosphorylation
T327 Phosphorylation
T330 Phosphorylation
T335 Phosphorylation
S408 Phosphorylation
S448 Phosphorylation
S453 Phosphorylation
Y494 Phosphorylation
S498 Phosphorylation
S500 Phosphorylation
T515 Phosphorylation
S519 Phosphorylation
S526 Phosphorylation
S548 Phosphorylation
S552 Phosphorylation
K553 Methylation
K554 Methylation
S564 Phosphorylation
S567 Phosphorylation
S569 Phosphorylation
S589 Phosphorylation
T590 Phosphorylation
S612 Phosphorylation
S614 Phosphorylation
T619 Phosphorylation
R635 Methylation
S655 Phosphorylation
S659 Phosphorylation
S689 Phosphorylation
T706 Phosphorylation
S713 Phosphorylation
S719 Phosphorylation
S724 Phosphorylation
S725 Phosphorylation
Y732 Phosphorylation
S734 Phosphorylation
S738 Phosphorylation
S746 Phosphorylation
S747 Phosphorylation
S754 Phosphorylation
S760 Phosphorylation
K794 Acetylation
K799 Acetylation
S814 Phosphorylation
S846 Phosphorylation
T849 Phosphorylation
S856 Phosphorylation
K865 Ubiquitination
S867 Phosphorylation
S872 Phosphorylation
S874 Phosphorylation
T885 Phosphorylation
K909 Acetylation
T911 Phosphorylation
S921 Phosphorylation
S929 Phosphorylation
S933 Phosphorylation
S936 Phosphorylation
S941 Phosphorylation
S951 Phosphorylation
T962 Phosphorylation
S963 Phosphorylation
S965 Phosphorylation
S975 Phosphorylation
T976 Phosphorylation
T989 Phosphorylation
S992 Phosphorylation
T994 Phosphorylation
S997 Phosphorylation
S998 Phosphorylation
C999 S-Nitrosylation
T1001 Phosphorylation
S1005 Phosphorylation
S1063 Phosphorylation
S1068 Phosphorylation
S1079 Phosphorylation
T1083 Phosphorylation
K1123 Ubiquitination
S1136 Phosphorylation
S1169 Phosphorylation
S1177 Phosphorylation
T1181 Phosphorylation
S1197 Phosphorylation
K1210 Ubiquitination
K1222 Ubiquitination
K1226 Ubiquitination
K1240 Ubiquitination
K1254 Ubiquitination
S1255 Phosphorylation
S1264 Phosphorylation

Research Backgrounds

Function:

May function in pre-mRNA splicing.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed in fetal brain and liver, poorly expressed in salivary gland, heart, skin and ovary. Expressed in colorectal carcinomas and ovarian cancers. Overexpressed in colorectal carcinomas as compared to normal colonic mucosa.

Subunit Structure:

Interacts with POLR2A.

Family&Domains:

Belongs to the splicing factor SR family.

Restrictive clause

 

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