TCFL5 Antibody - #AF9211

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Product: TCFL5 Antibody
Catalog: AF9211
Description: Rabbit polyclonal antibody to TCFL5
Application: WB IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 48kDa; 53kD(Calculated).
Uniprot: Q9UL49
RRID: AB_2843401

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
TCFL5 Antibody detects endogenous levels of total TCFL5.
RRID:
AB_2843401
Cite Format: Affinity Biosciences Cat# AF9211, RRID:AB_2843401.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHe82; CHA; Cha transcription factor; E2BP-1; E2BP1; Figlb; HPV-16 E2-binding protein 1; MGC46135; TCFL 5; TCFL5; TCFL5_HUMAN; transcription factor-like 5 (basic helix-loop-helix); Transcription factor-like 5 protein;

Immunogens

Immunogen:

A synthesized peptide derived from human TCFL5, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9UL49(TCFL5_HUMAN) >>Visit HPA database.

Gene(ID):
TCFL5(10732)
Expression:
Q9UL49 TCFL5_HUMAN:

Isoform 3 is testis specific. Isoform 2 is pancreas specific.

Sequence:
MSGPGPREPPPEAGAAGGEAAVEGAGGGDAALGEPGLSFTTTDLSLVEMTEVEYTQLQHILCSHMEAAADGELETRLNSALLAAAGPGAGAGGFAAGGQGGAAPVYPVLCPSALAADAPCLGHIDFQELRMMLLSEAGAAEKTSGGGDGARARADGAAKEGAGAAAAAAGPDGAPEARAKPAVRVRLEDRFNSIPAEPPPAPRGPEPPEPGGALNNLVTLIRHPSELMNVPLQQQNKCTALVKNKTAATTTALQFTYPLFTTNACSTSGNSNLSQTQSSSNSCSVLEAAKHQDIGLPRAFSFCYQQEIESTKQTLGSRNKVLPEQVWIKVGEAALCKQALKRNRSRMRQLDTNVERRALGEIQNVGEGATATQGAWQSSESSQANLGEQAQSGPQGGRSQRRERHNRMERDRRRRIRICCDELNLLVPFCNAETDKATTLQWTTAFLKYIQERHGDSLKKEFESVFCGKTGRRLKLTRPDSLVTCPAQGSLQSSPSMEIK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Chicken
91
Rabbit
91
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 3 is testis specific. Isoform 2 is pancreas specific.

References

1). Single cell epigenomic and transcriptomic analysis uncovers potential transcription factors regulating mitotic/meiotic switch. Cell death & disease, 2023 (PubMed: 36797258) [IF=8.1]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5: Identification of TFs in female germ cells associated with mitotic/meiotic switch. A Workflow of the selected framework of potential core TFs in meiotic initiation. B Final acquired core TFs of meiotic initiation. C Dot plot shows the expression trend of final acquired potential core TFs. The upper panel shows Pearson’s correlation between core TFs and Stra8. D Sankey diagram of TFs interaction network; Blue represents strong interactions, black represents weak interactions. E Function enrichment network of TCFL5 potential bound genes. F The epigenetic characteristics of Tcfl5; Volcano plot shows the gene activity score of Stra8 in cell subtypes. G TCFL5 immunolocalization on chromosomes of germ cells at different stages of meiotic prophase I; bar = 1 μm, n ≥ 11; Red shows SYCP3, green showsTCFL5, and blue shows nuclei; H The barplot showing the expression level of TCFL5 protein at different stages of meiotic prophase I. ** means p value 

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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