TLE4 Antibody - #AF9214
| Product: | TLE4 Antibody |
| Catalog: | AF9214 |
| Description: | Rabbit polyclonal antibody to TLE4 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 83kDa; 84kD(Calculated). |
| Uniprot: | Q04727 |
| RRID: | AB_2843404 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9214, RRID:AB_2843404.
Fold/Unfold
B lymphocyte gene 1; E(sp1) homolog Drosophila; E(spI); enhancer of split groucho 4; ESG; ESG4; GRG4; KIAA1261; TIM; TIM1; timeless (Drosophila) homolog; TLE4; Transducin like enhancer protein 4; transducin-like enhancer of split 4;
Immunogens
- Q04727 TLE4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIRDLSKMYPQTRHPAPHQPAQPFKFTISESCDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNAICAQVIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQAQHLSHGHGLPVPLTPHPSGLQPPAIPPIGSSAGLLALSSALGGQSHLPIKDEKKHHDNDHQRDRDSIKSSSVSPSASFRGAEKHRNSADYSSESKKQKTEEKEIAARYDSDGEKSDDNLVVDVSNEDPSSPRGSPAHSPRENGLDKTRLLKKDAPISPASIASSSSTPSSKSKELSLNEKSTTPVSKSNTPTPRTDAPTPGSNSTPGLRPVPGKPPGVDPLASSLRTPMAVPCPYPTPFGIVPHAGMNGELTSPGAAYAGLHNISPQMSAAAAAAAAAAAYGRSPVVGFDPHHHMRVPAIPPNLTGIPGGKPAYSFHVSADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCRLLPDGRTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMENSNVEVLHVTKPDKYQLHLHESCVLSLKFAHCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q04727 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| R13 | Methylation | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| Y225 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S227 | Phosphorylation | Uniprot | |
| K237 | Acetylation | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K281 | Acetylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| K308 | Acetylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K322 | Acetylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| T334 | Phosphorylation | Uniprot | |
| R431 | Methylation | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| R644 | Methylation | Uniprot | |
| Y695 | Phosphorylation | Uniprot | |
| S702 | Phosphorylation | Uniprot | |
| S706 | Phosphorylation | Uniprot | |
| K721 | Acetylation | Uniprot | |
| K721 | Ubiquitination | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| K765 | Sumoylation | Uniprot |
Research Backgrounds
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).
Phosphorylated. PAX5 binding increases phosphorylation (By similarity).
Ubiquitinated by XIAP/BIRC4.
Nucleus.
In all tissues examined, mostly in brain, and muscle.
Homooligomer and heterooligomer with other family members. Binds PAX5, LEF1, TCF7, TCF7L1 and TCF7L2. Interacts with ZNF703; TLE4 may mediate ZNF703 transcriptional repression. Interacts with SIX3 and SIX6 (By similarity). Interacts with PAX2.
WD repeat Groucho/TLE family members are characterized by 5 regions, a glutamine-rich Q domain, a glycine/proline-rich GP domain, a central CcN domain, containing a nuclear localization signal, and a serine/proline-rich SP domain. The most highly conserved are the N-terminal Q domain and the C-terminal WD-repeat domain.
Belongs to the WD repeat Groucho/TLE family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.