TRF1 Antibody - #AF9217
| Product: | TRF1 Antibody |
| Catalog: | AF9217 |
| Description: | Rabbit polyclonal antibody to TRF1 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Horse, Sheep |
| Mol.Wt.: | 50kDa; 50kD(Calculated). |
| Uniprot: | P54274 |
| RRID: | AB_2843407 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF9217, RRID:AB_2843407.
Fold/Unfold
hTRF1 AS; NIMA interacting protein 2; NIMA-interacting protein 2; PIN 2; PIN2; t TRF1; Telomeric protein Pin2; Telomeric protein Pin2/TRF1; Telomeric repeat binding factor (NIMA interacting) 1; Telomeric repeat binding factor 1; Telomeric repeat binding protein 1; Telomeric repeat-binding factor 1; TERF 1; Terf1; TERF1_HUMAN; TRBF 1; TRBF1; TRF 1; TRF; TTAGGG repeat binding factor 1; TTAGGG repeat-binding factor 1;
Immunogens
- P54274 TERF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEAEAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDAQFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPFKSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSGNDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKKKKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVMLKDRWRTMKKLKLISSDSED
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P54274 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Q8TDX7 (NEK7) | Uniprot |
| T122 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K136 | Ubiquitination | Uniprot | |
| T149 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S219 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K241 | Acetylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| T271 | Phosphorylation | Uniprot | |
| T273 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K295 | Acetylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| K300 | Acetylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| K319 | Ubiquitination | Uniprot | |
| K325 | Ubiquitination | Uniprot | |
| T330 | Phosphorylation | Uniprot | |
| T344 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S347 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T371 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K397 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S437 | Phosphorylation | Uniprot |
Research Backgrounds
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Nucleus. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Telomere.
Note: Colocalizes with telomeric DNA in interphase and prophase cells. Telomeric localization decreases in metaphase, anaphase and telophase. Associates with the mitotic spindle.
Highly expressed and ubiquitous. Isoform Pin2 predominates.
Homodimer; can contain both isoforms. Found in a complex with POT1; TINF2 and TNKS1. Interacts with ATM, TINF2, TNKS1, TNKS2, PINX1, NEK2 and MAPRE1. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Interacts with RLIM (via N-terminus). Interacts with FBXO4. Interaction with TINF2 protects against interaction with FBXO4 and subsequent polyubiquitination and proteasomal degradation. Interacts with GNL3L; this interaction promotes homodimerization. Interacts with TIN2. Interacts with RTEL1. Interactions with GNL3L and TIN2 are mutually exclusive. Interacts with CCDC79/TERB1 (By similarity).
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2.
The HTH domain is an independent structural unit and mediates binding to telomeric DNA.
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