Uba5 Antibody - #AF9220

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Product: Uba5 Antibody
Catalog: AF9220
Description: Rabbit polyclonal antibody to Uba5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 45kDa; 45kD(Calculated).
Uniprot: Q9GZZ9
RRID: AB_2843410

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(89%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
Uba5 Antibody detects endogenous levels of total Uba5.
RRID:
AB_2843410
Cite Format: Affinity Biosciences Cat# AF9220, RRID:AB_2843410.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5730525G14Rik; AW240750; FLJ23251; FLJ23251UBA5; MGC105565; ThiFP1; UBA5; UBA5 ubiquitin activating enzyme E1 homolog; UBA5_HUMAN; UBE1DC; UBE1DC1; Ubiquitin activating enzyme 5; Ubiquitin activating enzyme E1 domain containing 1; Ubiquitin activating enzyme E1 domain containing protein 1; Ubiquitin like modifier activating enzyme 5; Ubiquitin-activating enzyme 5; Ubiquitin-activating enzyme E1 domain-containing protein 1; Ubiquitin-like modifier-activating enzyme 5; UFM1 activating enzyme; UFM1-activating enzyme;

Immunogens

Immunogen:
Uniprot:

Q9GZZ9(UBA5_HUMAN) >>Visit HPA database.

Gene(ID):
UBA5(79876)
Expression:
Q9GZZ9 UBA5_HUMAN:

Widely expressed.

Sequence:
MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Bovine
89
Dog
89
Chicken
88
Xenopus
78
Zebrafish
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZZ9 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K42 Ubiquitination
S44 Phosphorylation
S45 Phosphorylation
Y53 Phosphorylation
S66 Phosphorylation
Y68 Phosphorylation
K70 Ubiquitination
K99 Ubiquitination
K107 Ubiquitination
K127 Ubiquitination
K173 Ubiquitination
S358 Phosphorylation
Y372 Phosphorylation
T373 Phosphorylation
K376 Ubiquitination
S381 Phosphorylation
T383 Phosphorylation
T386 Phosphorylation
S390 Phosphorylation
S393 Phosphorylation

Research Backgrounds

Function:

E1-like enzyme which activates UFM1 and SUMO2.

Subcellular Location:

Cytoplasm. Nucleus. Golgi apparatus.
Note: Localizes mainly in the cytoplasm, while it localizes to the nucleus in presence of SUMO2 (PubMed:18442052).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts (via C-terminus) with UFC1. Interacts with UFM1.

Family&Domains:

Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.

References

1). Heat-induced antigen retrieval utilizing modified Tris-EDTA buffer for reprobing of Western blots on nitrocellulose paper. Analytical Biochemistry, 2023 (PubMed: 37150424) [IF=2.6]

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