USP36 Antibody - #AF9223

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Product: USP36 Antibody
Catalog: AF9223
Description: Rabbit polyclonal antibody to USP36
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 125kDa; 123kD(Calculated).
Uniprot: Q9P275
RRID: AB_2843413

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(92%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
USP36 Antibody detects endogenous levels of total USP36.
RRID:
AB_2843413
Cite Format: Affinity Biosciences Cat# AF9223, RRID:AB_2843413.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Deubiquitinating enzyme 1; Deubiquitinating enzyme 36; DUB1; FLJ12851; KIAA1453; Ubiquitin carboxyl-terminal hydrolase 36; Ubiquitin specific peptidase 36; Ubiquitin specific processing protease 36; Ubiquitin thioesterase 36; Ubiquitin thiolesterase 36; Ubiquitin-specific-processing protease 36; UBP36_HUMAN; Usp36;

Immunogens

Immunogen:
Uniprot:

Q9P275(UBP36_HUMAN) >>Visit HPA database.

Gene(ID):
USP36(57602)
Expression:
Q9P275 UBP36_HUMAN:

Broadly expressed.

Sequence:
MPIVDKLKEALKPGRKDSADDGELGKLLASSAKKVLLQKIEFEPASKSFSYQLEALKSKYVLLNPKTEGASRHKSGDDPPARRQGSEHTYESCGDGVPAPQKVLFPTERLSLRWERVFRVGAGLHNLGNTCFLNATIQCLTYTPPLANYLLSKEHARSCHQGSFCMLCVMQNHIVQAFANSGNAIKPVSFIRDLKKIARHFRFGNQEDAHEFLRYTIDAMQKACLNGCAKLDRQTQATTLVHQIFGGYLRSRVKCSVCKSVSDTYDPYLDVALEIRQAANIVRALELFVKADVLSGENAYMCAKCKKKVPASKRFTIHRTSNVLTLSLKRFANFSGGKITKDVGYPEFLNIRPYMSQNNGDPVMYGLYAVLVHSGYSCHAGHYYCYVKASNGQWYQMNDSLVHSSNVKVVLNQQAYVLFYLRIPGSKKSPEGLISRTGSSSLPGRPSVIPDHSKKNIGNGIISSPLTGKRQDSGTMKKPHTTEEIGVPISRNGSTLGLKSQNGCIPPKLPSGSPSPKLSQTPTHMPTILDDPGKKVKKPAPPQHFSPRTAQGLPGTSNSNSSRSGSQRQGSWDSRDVVLSTSPKLLATATANGHGLKGNDESAGLDRRGSSSSSPEHSASSDSTKAPQTPRSGAAHLCDSQETNCSTAGHSKTPPSGADSKTVKLKSPVLSNTTTEPASTMSPPPAKKLALSAKKASTLWRATGNDLRPPPPSPSSDLTHPMKTSHPVVASTWPVHRARAVSPAPQSSSRLQPPFSPHPTLLSSTPKPPGTSEPRSCSSISTALPQVNEDLVSLPHQLPEASEPPQSPSEKRKKTFVGEPQRLGSETRLPQHIREATAAPHGKRKRKKKKRPEDTAASALQEGQTQRQPGSPMYRREGQAQLPAVRRQEDGTQPQVNGQQVGCVTDGHHASSRKRRRKGAEGLGEEGGLHQDPLRHSCSPMGDGDPEAMEESPRKKKKKKRKQETQRAVEEDGHLKCPRSAKPQDAVVPESSSCAPSANGWCPGDRMGLSQAPPVSWNGERESDVVQELLKYSSDKAYGRKVLTWDGKMSAVSQDAIEDSRQARTETVVDDWDEEFDRGKEKKIKKFKREKRRNFNAFQKLQTRRNFWSVTHPAKAASLSYRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Pig
92
Xenopus
92
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P275 As Substrate

Site PTM Type Enzyme
S18 Phosphorylation
K26 Ubiquitination
S31 Phosphorylation
K33 Ubiquitination
K47 Ubiquitination
S50 Phosphorylation
K230 Acetylation
K230 Ubiquitination
Y268 Phosphorylation
K304 Ubiquitination
K329 Ubiquitination
K338 Ubiquitination
K341 Ubiquitination
K428 Ubiquitination
S429 Phosphorylation
S439 Phosphorylation
S447 Phosphorylation
S453 Phosphorylation
S464 Phosphorylation
T467 Phosphorylation
S494 Phosphorylation
K508 Methylation
S513 Phosphorylation
S515 Phosphorylation
T521 Phosphorylation
T523 Phosphorylation
S546 Phosphorylation
S571 Phosphorylation
S580 Phosphorylation
T581 Phosphorylation
S582 Phosphorylation
S602 Phosphorylation
S610 Phosphorylation
S612 Phosphorylation
S613 Phosphorylation
S614 Phosphorylation
S618 Phosphorylation
S620 Phosphorylation
S621 Phosphorylation
T629 Phosphorylation
S632 Phosphorylation
S640 Phosphorylation
S651 Phosphorylation
T653 Phosphorylation
S656 Phosphorylation
S667 Phosphorylation
S671 Phosphorylation
T675 Phosphorylation
S679 Phosphorylation
T680 Phosphorylation
S682 Phosphorylation
S692 Phosphorylation
S713 Phosphorylation
S742 Phosphorylation
S748 Phosphorylation
S756 Phosphorylation
T760 Phosphorylation
S763 Phosphorylation
S764 Phosphorylation
T765 Phosphorylation
S807 Phosphorylation
S825 Phosphorylation
S871 Phosphorylation
S937 Phosphorylation
S939 Phosphorylation
S952 Phosphorylation
S1118 Phosphorylation

Research Backgrounds

Function:

Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability. Functions as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4). Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm. Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions. Deubiquitinates SOD2, regulates SOD2 protein stability. Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins.

PTMs:

Polyubiquitinated by NEDD4L, no effect on USP36 protein levels. Both proteins interact with and regulate each other's ubiquitination levels.

Subcellular Location:

Nucleus>Nucleolus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Broadly expressed.

Subunit Structure:

Interacts with isoform 3 of FBXW7; the interaction inhibits MYC degradation induced by SCF(FBW7) complex. Interacts with NTRK1; USP36 does not deubiquitinate NTRK1. Interacts with NEDD4L (via domains WW1, 3 and 4); the interaction inhibits ubiquitination of, at least, NTRK1, KCNQ2 and KCNQ3 by NEDD4L.

Family&Domains:

Belongs to the peptidase C19 family.

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