USP50 Antibody - #AF9225
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| Product: | USP50 Antibody |
| Catalog: | AF9225 |
| Description: | Rabbit polyclonal antibody to USP50 |
| Application: | WB |
| Cited expt.: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Horse, Rabbit |
| Mol.Wt.: | 38kDa; 39kD(Calculated). |
| Uniprot: | Q70EL3 |
| RRID: | AB_2843415 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Horse(88%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
USP50 Antibody detects endogenous levels of total USP50.
RRID:
AB_2843415
Cite Format: Affinity Biosciences Cat# AF9225, RRID:AB_2843415.
Cite Format: Affinity Biosciences Cat# AF9225, RRID:AB_2843415.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Inactive ubiquitin specific peptidase 50; Ubiquitin specific peptidase 50; Ubiquitin specific protease 50; USP50;
Immunogens
Immunogen:
A synthesized peptide derived from human USP50, corresponding to a region within the internal amino acids.
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q70EL3 UBP50_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSQPSLPADDFDIYHVLAECTDYYDTLPVKEADGNQPHFQGVTGLWNLGNTCCVNAISQCLCSILPLVEYFLTGKYITALQKFLLPSDCSEVATAFAYLMTDMWLGDSDCVSPEIFWSALGNLYPAFTKKMQQDAQEFLICVLNELHEALKKYHYSRRRSYEKGSTQRCCRKWITTETSIITQLFEEQLNYSIVCLKCEKCTYKNEVFTVFSLPIPSKYECSLRDCLQCFFQQDALTWNNEIHCSFCETKQETAVRASISKAPKIIIFHLKRFDIQGTTKRKLRTDIHYPLTNLDLTPYICSIFRKYPKYNLCAVVNHFGDLDGGHYTAFCKNSVTQA
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Horse
88
Pig
75
Sheep
75
Dog
75
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Function:
Has no peptidase activity.
Tissue Specificity:
Weakly expressed in a few tissues.
Family&Domains:
Belongs to the peptidase C19 family.
References
1). Vitamin C promotes ACE2 degradation and protects against SARS-CoV-2 infection. EMBO reports, 2023
(PubMed: 36876523)
[IF=6.5]
Application: WB Species: human Sample: HEK293T cells
Figure 3. VitC‐induced reduction of ACE2 levels largely depends on the deubiquitinase USP50 A. Western blot analysis of ACE2 in HEK293T cells pretreated with PR619 (50 μM, 2 h) and then treated with VitC (5 mM) for 12 h. Data are representative of three biological replicates. B. HEK293T cells were individually transfected with the plasmids from the human DUBs expression library. Western blot was used to identify the key deubiquitinase that significantly increases ACE2 levels. Data were from two biological replicates and were shown as the fold change (the intensity of ACE2/Tubulin bands measured by the Image J) normalized to the empty vector group (CON). C. IP‐IB analysis of the interaction between Flag‐USP50 and Myc‐ACE2 in HEK293T cells cotransfected with these two constructs. Data are representative of three biological replicates. D. Immunoprecipitation analysis of the interaction between endogenous USP50 and ACE2 in 2fTGH cells. Data are representative of three biological replicates. E. Western blot analysis of ACE2 in HEK293T cells transfected with increasing amounts of Flag‐USP50. Data are representative of three biological replicates. F. Western blot analysis of ACE2 in HEK293T cells transfected with shCtrl or shUSP50 (#1 or #2). Data are representative of three biological replicates. G. Western blot analysis of ACE2 in HEK293T cells transfected with Flag‐USP50 and then treated with CHX (50 μM) as indicated. Data are representative of three biological replicates. H. Western blot analysis of ACE2 in Usp50+/+ and Usp50−/− HEK293T cells. Data are representative of three biological replicates. I. Western blot analysis of ACE2 in Usp50+/+ and Usp50−/− HEK293T cells treated with VitC (5 mM) for 12 h. Data are representative of three biological replicates. J. RT–qPCR analysis of SARS‐CoV‐2 GFP/ΔN RNA levels in HEK293T cells transfected with Flag‐USP50 and then infected with the SARS‐CoV‐2 GFP/ΔN virus (MOI = 0.1) for 2 h. Data are shown as mean and s.d. of three biological replicates (n = 3). *P
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