USP50 Antibody - #AF9225
(1)
| Product: | USP50 Antibody |
| Catalog: | AF9225 |
| Description: | Rabbit polyclonal antibody to USP50 |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Prediction: | Horse, Rabbit |
| Mol.Wt.: | 38kDa; 39kD(Calculated). |
| Uniprot: | Q70EL3 |
| RRID: | AB_2843415 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse
Prediction:
Horse(88%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
USP50 Antibody detects endogenous levels of total USP50.
RRID:
AB_2843415
Cite Format: Affinity Biosciences Cat# AF9225, RRID:AB_2843415.
Cite Format: Affinity Biosciences Cat# AF9225, RRID:AB_2843415.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Inactive ubiquitin specific peptidase 50; Ubiquitin specific peptidase 50; Ubiquitin specific protease 50; USP50;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q70EL3 UBP50_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSQPSLPADDFDIYHVLAECTDYYDTLPVKEADGNQPHFQGVTGLWNLGNTCCVNAISQCLCSILPLVEYFLTGKYITALQKFLLPSDCSEVATAFAYLMTDMWLGDSDCVSPEIFWSALGNLYPAFTKKMQQDAQEFLICVLNELHEALKKYHYSRRRSYEKGSTQRCCRKWITTETSIITQLFEEQLNYSIVCLKCEKCTYKNEVFTVFSLPIPSKYECSLRDCLQCFFQQDALTWNNEIHCSFCETKQETAVRASISKAPKIIIFHLKRFDIQGTTKRKLRTDIHYPLTNLDLTPYICSIFRKYPKYNLCAVVNHFGDLDGGHYTAFCKNSVTQA
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Horse
88
Pig
75
Sheep
75
Dog
75
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q70EL3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y24 | Phosphorylation | Uniprot | |
| Y25 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Has no peptidase activity.
Tissue Specificity:
Weakly expressed in a few tissues.
Family&Domains:
Belongs to the peptidase C19 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.