WAVE1 Antibody - #AF9229

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Product: WAVE1 Antibody
Catalog: AF9229
Description: Rabbit polyclonal antibody to WAVE1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 70kDa; 62kD(Calculated).
Uniprot: Q92558
RRID: AB_2843419

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
WAVE1 Antibody detects endogenous levels of total WAVE1.
RRID:
AB_2843419
Cite Format: Affinity Biosciences Cat# AF9229, RRID:AB_2843419.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

;WASP family, verprolin homology domain-containing protein 1; WASP family member 1; FLJ31482; homology of dictyostelium scar 1; KIAA0269; Protein WAVE-1; Protein WAVE1; scar, Dictyostelium, homology of, 1; SCAR1; Similar to a plant extensin like protein; Verprolin homology domain containing protein 1; Verprolin homology domain-containing protein 1; WAS protein family, member 1; Wasf1; WASF1_HUMAN; WASL; WASP family 1; WASP family protein member 1; WASP family, verprolin homology domain-containing protein; WAVE; WAVE1; Wiskott Aldrich syndrome protein family member 1; Wiskott-Aldrich syndrome protein family member 1;

Immunogens

Immunogen:
Uniprot:

Q92558(WASF1_HUMAN) >>Visit HPA database.

Gene(ID):
WASF1(8936)
Expression:
Q92558 WASF1_HUMAN:

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Sequence:
MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPYRDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQGPELAEDDANLLHKHIEVANGPASHFETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPPPPPMHGAGDAKPIPTCISSATGLIENRPQSPATGRTPVFVSPTPPPPPPPLPSALSTSSLRASMTSTPPPPVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEVQGLPPPPPPPPLPPPGIRPSSPVTVTALAHPPSGLHPTPSTAPGPHVPLMPPSPPSQVIPASEPKRHPSTLPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
86
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92558 As Substrate

Site PTM Type Enzyme
S91 Phosphorylation
T96 Phosphorylation
Y125 Phosphorylation P12931 (SRC)
Y151 Phosphorylation
T169 Phosphorylation
K182 Acetylation
K192 Acetylation
S298 Phosphorylation
R307 Methylation
S310 Phosphorylation Q00535 (CDK5)
R315 Methylation
S321 Phosphorylation
T323 Phosphorylation
S339 Phosphorylation
S397 Phosphorylation Q00535 (CDK5)
S441 Phosphorylation Q00535 (CDK5)
S489 Phosphorylation
T490 Phosphorylation
Y543 Phosphorylation
S546 Phosphorylation
S550 Phosphorylation

Research Backgrounds

Function:

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity).

PTMs:

Phosphorylated on tyrosine residues by ABL1 and dephosphorylated by PTPN12.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Synapse. Cell junction>Focal adhesion.
Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (PubMed:9889097). Partial translocation to focal adhesion sites might be mediated by interaction with SORBS2 (PubMed:18559503). In neurons, colocalizes with activated NTRK2 after BDNF addition in endocytic sites through the association with TMEM108 (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.

Subunit Structure:

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.

Family&Domains:

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.

Belongs to the SCAR/WAVE family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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