WDHD1 Antibody - #AF9232

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Product: WDHD1 Antibody
Catalog: AF9232
Description: Rabbit polyclonal antibody to WDHD1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 126kDa; 126kD(Calculated).
Uniprot: O75717
RRID: AB_2843422

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
WDHD1 Antibody detects endogenous levels of total WDHD1.
RRID:
AB_2843422
Cite Format: Affinity Biosciences Cat# AF9232, RRID:AB_2843422.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acidic nucleoplasmic DNA binding protein 1; And 1; And1; WD repeat and HMG box DNA binding protein 1; WDHD 1;

Immunogens

Immunogen:
Uniprot:

O75717(WDHD1_HUMAN) >>Visit HPA database.

Gene(ID):
WDHD1(11169)
Sequence:
MPATRKPMRYGHTEGHTEVCFDDSGSFIVTCGSDGDVRIWEDLDDDDPKFINVGEKAYSCALKSGKLVTAVSNNTIQVHTFPEGVPDGILTRFTTNANHVVFNGDGTKIAAGSSDFLVKIVDVMDSSQQKTFRGHDAPVLSLSFDPKDIFLASASCDGSVRVWQISDQTCAISWPLLQKCNDVINAKSICRLAWQPKSGKLLAIPVEKSVKLYRRESWSHQFDLSDNFISQTLNIVTWSPCGQYLAAGSINGLIIVWNVETKDCMERVKHEKGYAICGLAWHPTCGRISYTDAEGNLGLLENVCDPSGKTSSSKVSSRVEKDYNDLFDGDDMSNAGDFLNDNAVEIPSFSKGIINDDEDDEDLMMASGRPRQRSHILEDDENSVDISMLKTGSSLLKEEEEDGQEGSIHNLPLVTSQRPFYDGPMPTPRQKPFQSGSTPLHLTHRFMVWNSIGIIRCYNDEQDNAIDVEFHDTSIHHATHLSNTLNYTIADLSHEAILLACESTDELASKLHCLHFSSWDSSKEWIIDLPQNEDIEAICLGQGWAAAATSALLLRLFTIGGVQKEVFSLAGPVVSMAGHGEQLFIVYHRGTGFDGDQCLGVQLLELGKKKKQILHGDPLPLTRKSYLAWIGFSAEGTPCYVDSEGIVRMLNRGLGNTWTPICNTREHCKGKSDHYWVVGIHENPQQLRCIPCKGSRFPPTLPRPAVAILSFKLPYCQIATEKGQMEEQFWRSVIFHNHLDYLAKNGYEYEESTKNQATKEQQELLMKMLALSCKLEREFRCVELADLMTQNAVNLAIKYASRSRKLILAQKLSELAVEKAAELTATQVEEEEEEEDFRKKLNAGYSNTATEWSQPRFRNQVEEDAEDSGEADDEEKPEIHKPGQNSFSKSTNSSDVSAKSGAVTFSSQGRVNPFKVSASSKEPAMSMNSARSTNILDNMGKSSKKSTALSRTTNNEKSPIIKPLIPKPKPKQASAASYFQKRNSQTNKTEEVKEENLKNVLSETPAICPPQNTENQRPKTGFQMWLEENRSNILSDNPDFSDEADIIKEGMIRFRVLSTEERKVWANKAKGETASEGTEAKKRKRVVDESDETENQEEKAKENLNLSKKQKPLDFSTNQKLSAFAFKQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75717 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
T30 Phosphorylation
S33 Phosphorylation
K49 Ubiquitination
K56 Ubiquitination
K63 Ubiquitination
K66 Ubiquitination
K108 Ubiquitination
K119 Ubiquitination
S126 Phosphorylation
S127 Phosphorylation
K130 Ubiquitination
T131 Phosphorylation
K187 Ubiquitination
K197 Ubiquitination
K200 Ubiquitination
K208 Ubiquitination
K272 Ubiquitination
S307 Phosphorylation
K309 Ubiquitination
K314 Ubiquitination
Y323 Phosphorylation
S333 Phosphorylation
S348 Phosphorylation
S350 Phosphorylation
S367 Phosphorylation
S374 Phosphorylation
S383 Phosphorylation
S387 Phosphorylation
K390 Ubiquitination
S393 Phosphorylation
S394 Phosphorylation
K397 Sumoylation
S407 Phosphorylation
T591 Phosphorylation
K611 Ubiquitination
K671 Acetylation
K744 Ubiquitination
K759 Ubiquitination
K767 Ubiquitination
K774 Ubiquitination
Y799 Phosphorylation
S801 Phosphorylation
S803 Phosphorylation
K805 Ubiquitination
K811 Ubiquitination
S813 Phosphorylation
K819 Ubiquitination
T824 Phosphorylation
T826 Phosphorylation Q13535 (ATR)
K840 Sumoylation
K840 Ubiquitination
S846 Phosphorylation
T848 Phosphorylation
S853 Phosphorylation
S868 Phosphorylation
S886 Phosphorylation
K899 Ubiquitination
S906 Phosphorylation
S907 Phosphorylation
K915 Methylation
K915 Ubiquitination
S917 Phosphorylation
S919 Phosphorylation
S926 Phosphorylation
S932 Phosphorylation
S958 Phosphorylation
K962 Acetylation
K971 Ubiquitination
S977 Phosphorylation
Y978 Phosphorylation
S984 Phosphorylation
T986 Phosphorylation
S1041 Phosphorylation
S1058 Phosphorylation
K1063 Acetylation
K1068 Acetylation
K1070 Acetylation
S1090 Phosphorylation
K1101 Acetylation
K1127 Acetylation
K1127 Sumoylation

Research Backgrounds

Function:

Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication.

Subcellular Location:

Nucleus>Nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with the polymerase alpha catalytic subunit POLA1. Interacts with MCM10. Interacts with DNA2.

Restrictive clause

 

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