YEATS2 Antibody | Affinity Biosciences

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YEATS2 Antibody - Western blot analysis of extracts from Raji cells, using YEATS2 Antibody.

Product:	YEATS2 Antibody
Catalog:	AF9233
Description:	Rabbit polyclonal antibody to YEATS2
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	150kDa; 151kD(Calculated).
Uniprot:	Q9ULM3
RRID:	AB_2843423

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%), Bovine(100%), Horse(88%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

YEATS2 Antibody detects endogenous levels of total YEATS2.

RRID:

AB_2843423
Cite Format: Affinity Biosciences Cat# AF9233, RRID:AB_2843423.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ10201; FLJ12841; FLJ13308; FLJ33401; FLJ34335; KIAA1197; YEATS domain containing 2; YEATS domain-containing protein 2; Yeats2; YETS2_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9ULM3(YETS2_HUMAN) >>Visit HPA database.

Gene(ID):

YEATS2(55689)

Sequence:

Q9ULM3 YETS2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGIKRTIKETDPDYEDVSVALPNKRHKAIENSARDAAVQKIETIIKEQFALEMKNKEHEIEVIDQRLIEARRMMDKLRACIVANYYASAGLLKVSEGSKTCDTMVFNHPAIKKFLESPSRSSSPANQRAETPSANHSESDSLSQHNDFLSDKDNNSNMDIEERLSNNMEQRPSRNTGRDTSRITGSHKTEQRNADLTDETSRLFVKKTIVVGNVSKYIPPDKREENDQSTHKWMVYVRGSRREPSINHFVKKVWFFLHPSYKPNDLVEVREPPFHLTRRGWGEFPVRVQVHFKDSQNKRIDIIHNLKLDRTYTGLQTLGAETVVDVELHRHSLGEDCIYPQSSESDISDAPPSLPLTIPAPVKASSPIKQSHEPVPDTSVEKGFPASTEAERHTPFYALPSSLERTPTKMTTSQKVTFCSHGNSAFQPIASSCKIVPQSQVPNPESPGKSFQPITMSCKIVSGSPISTPSPSPLPRTPTSTPVHVKQGTAGSVINNPYVIMDKQPGQVIGATTPSTGSPTNKISTASQVSQGTGSPVPKIHGSSFVTSTVKQEDSLFASMPPLCPIGSHPKVQSPKPITGGLGAFTKVIIKQEPGEAPHVPATGAASQSPLPQYVTVKGGHMIAVSPQKQVITPGEGIAQSAKVQPSKVVGVPVGSALPSTVKQAVAISGGQILVAKASSSVSKAVGPKQVVTQGVAKAIVSGGGGTIVAQPVQTLTKAQVTAAGPQKSGSQGSVMATLQLPATNLANLANLPPGTKLYLTTNSKNPSGKGKLLLIPQGAILRATNNANLQSGSAASGGSGAGGGGGGGGGGGSGSGGGGSTGGGGGTAGGGTQSTAGPGGISQHLTYTSYILKQTPQGTFLVGQPSPQTSGKQLTTGSVVQGTLGVSTSSAQGQQTLKVISGQKTTLFTQAAHGGQASLMKISDSTLKTVPATSQLSKPGTTMLRVAGGVITTATSPAVALSANGPAQQSEGMAPVSSSTVSSVTKTSGQQQVCVSQATVGTCKAATPTVVSATSLVPTPNPISGKATVSGLLKIHSSQSSPQQAVLTIPSQLKPLSVNTSGGVQTILMPVNKVVQSFSTSKPPAILPVAAPTPVVPSSAPAAVAKVKTEPETPGPSCLSQEGQTAVKTEESSELGNYVIKIDHLETIQQLLTAVVKKIPLITAKSEDASCFSAKSVEQYYGWNIGKRRAAEWQRAMTMRKVLQEILEKNPRFHHLTPLKTKHIAHWCRCHGYTPPDPESLRNDGDSIEDVLTQIDSEPECPSSFSSADNLCRKLEDLQQFQKREPENEEEVDILSLSEPVKINIKKEQEEKQEEVKFYLPPTPGSEFIGDVTQKIGITLQPVALHRNVYASVVEDMILKATEQLVNDILRQALAVGYQTASHNRIPKEITVSNIHQAICNIPFLDFLTNKHMGILNEDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Pig

Horse

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULM3 As Substrate

Q9ULM3

Site	PTM Type	Source
T11	Phosphorylation	Uniprot
Y15	Phosphorylation	Uniprot
K25	Ubiquitination	Uniprot
K28	Ubiquitination	Uniprot
K41	Ubiquitination	Uniprot
K57	Acetylation	Uniprot
K100	Ubiquitination	Uniprot
S118	Phosphorylation	Uniprot
S120	Phosphorylation	Uniprot
S122	Phosphorylation	Uniprot
S123	Phosphorylation	Uniprot
S124	Phosphorylation	Uniprot
T132	Phosphorylation	Uniprot
S134	Phosphorylation	Uniprot
S144	Phosphorylation	Uniprot
S157	Phosphorylation	Uniprot
T209	Phosphorylation	Uniprot
S216	Phosphorylation	Uniprot
S246	Phosphorylation	Uniprot
K308	Ubiquitination	Uniprot
S366	Phosphorylation	Uniprot
S367	Phosphorylation	Uniprot
T379	Phosphorylation	Uniprot
S380	Phosphorylation	Uniprot
K383	Ubiquitination	Uniprot
T395	Phosphorylation	Uniprot
S402	Phosphorylation	Uniprot
S403	Phosphorylation	Uniprot
T407	Phosphorylation	Uniprot
T409	Phosphorylation	Uniprot
S447	Phosphorylation	Uniprot
K450	Acetylation	Uniprot
S451	Phosphorylation	Uniprot
S463	Phosphorylation	Uniprot
S465	Phosphorylation	Uniprot
S468	Phosphorylation	Uniprot
T469	Phosphorylation	Uniprot
S471	Phosphorylation	Uniprot
S473	Phosphorylation	Uniprot
T478	Phosphorylation	Uniprot
T480	Phosphorylation	Uniprot
S481	Phosphorylation	Uniprot
T482	Phosphorylation	Uniprot
T514	Phosphorylation	Uniprot
S516	Phosphorylation	Uniprot
S519	Phosphorylation	Uniprot
T521	Phosphorylation	Uniprot
S531	Phosphorylation	Uniprot
T534	Phosphorylation	Uniprot
S536	Phosphorylation	Uniprot
K552	Sumoylation	Uniprot
S575	Phosphorylation	Uniprot
K592	Sumoylation	Uniprot
S610	Phosphorylation	Uniprot
S627	Phosphorylation	Uniprot
T634	Phosphorylation	Uniprot
K644	Ubiquitination	Uniprot
K649	Ubiquitination	Uniprot
S657	Phosphorylation	Uniprot
K685	Ubiquitination	Uniprot
K690	Ubiquitination	Uniprot
K719	Ubiquitination	Uniprot
S765	Phosphorylation	Uniprot
K766	Ubiquitination	Uniprot
K773	Sumoylation	Uniprot
K773	Ubiquitination	Uniprot
S868	Phosphorylation	Uniprot
S903	Phosphorylation	Uniprot
T911	Phosphorylation	Uniprot
K923	Ubiquitination	Uniprot
K930	Ubiquitination	Uniprot
K940	Ubiquitination	Uniprot
T1021	Phosphorylation	Uniprot
S1039	Phosphorylation	Uniprot
S1040	Phosphorylation	Uniprot
S1043	Phosphorylation	Uniprot
T1068	Phosphorylation	Uniprot
K1084	Ubiquitination	Uniprot
K1110	Sumoylation	Uniprot
T1115	Phosphorylation	Uniprot
S1122	Phosphorylation	Uniprot
T1131	Phosphorylation	Uniprot
S1134	Phosphorylation	Uniprot
S1135	Phosphorylation	Uniprot
T1165	Phosphorylation	Uniprot
S1168	Phosphorylation	Uniprot
S1172	Phosphorylation	Uniprot
T1219	Phosphorylation	Uniprot
K1222	Ubiquitination	Uniprot
S1259	Phosphorylation	Uniprot
K1276	Ubiquitination	Uniprot
K1285	Ubiquitination	Uniprot
K1304	Ubiquitination	Uniprot
K1308	Acetylation	Uniprot
K1309	Acetylation	Uniprot

Research Backgrounds

Q9ULM3_YETS2_HUMAN

Function:

Chromatin reader component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. YEATS2 specifically recognizes and binds histone H3 crotonylated at 'Lys-27' (H3K27cr). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors.

Subcellular Location:

Nucleus.

Subunit Structure:

Component of the ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, SGF29 and DR1.

Family&Domains:

The YEATS domain specifically recognizes and binds crotonylated histones.

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For Research Use Only.
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YEATS2 Antibody - #AF9233