Phospho-SKI (Ser383) Antibody - #AF7036

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Product: Phospho-SKI (Ser383) Antibody
Catalog: AF7036
Description: Rabbit polyclonal antibody to Phospho-SKI (Ser383)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Dog, Chicken, Xenopus
Mol.Wt.: 80kDa; 80kD(Calculated).
Uniprot: P12755
RRID: AB_2843476

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Phospho-SKI (Ser383) Antibody detects endogenous levels of SKI only when phosphorylated at Ser383.
RRID:
AB_2843476
Cite Format: Affinity Biosciences Cat# AF7036, RRID:AB_2843476.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Avian sarcoma viral (v ski) oncogene homolog; C oncogene; C ski; Proto-oncogene c-Ski; SKI; Ski oncogene; Ski oncoprotein; SKI_HUMAN; SKV; v ski avian sarcoma viral oncogene homolog; v ski sarcoma viral oncogene homolog;

Immunogens

Immunogen:
Uniprot:

P12755(SKI_HUMAN) >>Visit HPA database.

Gene(ID):
SKI(6497)
Sequence:
MEAAAGGRGCFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAAAVPAPVPAATEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINAVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQARLGRCLDDVKEKFDYGNKYKRRVPRVSSEPPASIRPKTDDTSSQSPAPSEKDKPSSWLRTLAGSSNKSLGCVHPRQRLSAFRPWSPAVSASEKELSPHLPALIRDSFYSYKSFETAVAPNVALAPPAQQKVVSSPPCAAAVSRAPEPLATCTQPRKRKLTVDTPGAPETLAPVAAPEEDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLGSPGARALPSAVPDAAAPADAPSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLSAALQAKRSLHQELEFLRVAKKEKLREATEAKRNLRKEIERLRAENEKKMKEANESRLRLKRELEQARQARVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLLREREAREHLEKVVKELQEQLWPRARPEAAGSEGAAELEP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Chicken
100
Xenopus
90
Pig
0
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12755 As Substrate

Site PTM Type Enzyme
T103 Phosphorylation
S214 Phosphorylation
K258 Ubiquitination
K294 Ubiquitination
K316 Ubiquitination
K318 Acetylation
S325 Phosphorylation
S326 Phosphorylation O14965 (AURKA)
K335 Acetylation
S343 Phosphorylation
K351 Ubiquitination
S354 Phosphorylation
K365 Ubiquitination
S366 Phosphorylation
S377 Phosphorylation
S383 Phosphorylation O14965 (AURKA)
S394 Phosphorylation
S404 Phosphorylation
K409 Ubiquitination
S431 Phosphorylation
S432 Phosphorylation
T458 Phosphorylation P31749 (AKT1)
S480 Phosphorylation
S500 Phosphorylation
S515 Phosphorylation
K584 Ubiquitination
K662 Ubiquitination
K673 Ubiquitination
S720 Phosphorylation

Research Backgrounds

Function:

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.

PTMs:

Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Interacts with PRDM16 and SMAD3; the interaction with PRDM16 promotes the recruitment SMAD3-HDAC1 complex on the promoter of TGF-beta target genes.

Family&Domains:

Belongs to the SKI family.

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