Phospho-Synapsin I (Ser9) Antibody | Affinity Biosciences

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Phospho-Synapsin I (Ser9) Antibody - AF3201 at 1/100 staining Mouse brain tissue by IHC-P.

Phospho-Synapsin I (Ser9) Antibody - peptide-ELISA analysis of AF3201.

Phospho-Synapsin I (Ser9) Antibody - FIGURE 5 | Ubiquitin and synaptic protein expression changed in isoflurane-exposed aged mice.

Product:	Phospho-Synapsin I (Ser9) Antibody
Catalog:	AF3201
Description:	Rabbit polyclonal antibody to Phospho-Synapsin I (Ser9)
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Sheep, Rabbit, Xenopus
Mol.Wt.:	77kDa; 74kD(Calculated).
Uniprot:	P17600
RRID:	AB_2834633

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-Synapsin I (Ser9) Antibody detects endogenous levels of Synapsin I only when phosphorylated at Serine 9.

RRID:

AB_2834633
Cite Format: Affinity Biosciences Cat# AF3201, RRID:AB_2834633.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Brain protein 4.1; SYN 1; SYN 1a; SYN 1b; SYN I; SYN1; SYN1_HUMAN; SYN1a; SYN1b; Synapsin 1; Synapsin I; Synapsin-1; Synapsin1; SynapsinI; SYNI;

Immunogens

Immunogen:

A synthesized peptide derived from human Synapsin I around the phosphorylation site of Ser9.

Uniprot:

P17600(SYN1_HUMAN) >>Visit HPA database.

Gene(ID):

SYN1(6853)

Description:

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases.

Sequence:

P17600 SYN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNYLRRRLSDSNFMANLPNGYMTDLQRPQPPPPPPGAHSPGATPGPGTATAERSSGVAPAASPAAPSPGSSGGGGFFSSLSNAVKQTTAAAAATFSEQVGGGSGGAGRGGAASRVLLVIDEPHTDWAKYFKGKKIHGEIDIKVEQAEFSDLNLVAHANGGFSVDMEVLRNGVKVVRSLKPDFVLIRQHAFSMARNGDYRSLVIGLQYAGIPSVNSLHSVYNFCDKPWVFAQMVRLHKKLGTEEFPLIDQTFYPNHKEMLSSTTYPVVVKMGHAHSGMGKVKVDNQHDFQDIASVVALTKTYATAEPFIDAKYDVRVQKIGQNYKAYMRTSVSGNWKTNTGSAMLEQIAMSDRYKLWVDTCSEIFGGLDICAVEALHGKDGRDHIIEVVGSSMPLIGDHQDEDKQLIVELVVNKMAQALPRQRQRDASPGRGSHGQTPSPGALPLGRQTSQQPAGPPAQQRPPPQGGPPQPGPGPQRQGPPLQQRPPPQGQQHLSGLGPPAGSPLPQRLPSPTSAPQQPASQAAPPTQGQGRQSRPVAGGPGAPPAARPPASPSPQRQAGPPQATRQTSVSGPAPPKASGAPPGGQQRQGPPQKPPGPAGPTRQASQAGPVPRTGPPTTQQPRPSGPGPAGRPKPQLAQKPSQDVPPPATAAAGGPPHPQLNKSQSLTNAFNLPEPAPPRPSLSQDEVKAETIRSLRKSFASLFSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Xenopus

100

Zebrafish

100

Rabbit

100

Horse

Dog

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P17600_SYN1_HUMAN

Function:

Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

PTMs:

Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal.

Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles.

Subcellular Location:

Cell junction>Synapse. Golgi apparatus.

Family&Domains:

The A region binds phospholipids with a preference for negatively charged species.

Belongs to the synapsin family.

References

1). CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice. Frontiers in Neuroscience, 2022 (PubMed: 35368262) [IF=4.3]

Application: WB Species: mouse Sample: brain

FIGURE 5 | Ubiquitin and synaptic protein expression changed in isoflurane-exposed aged mice. (B) In the brain tissue of cortex,there was no significant difference in the expression level of synapsin I and the phosphorylation level of synapsin I S9 and S605 between CON group, ISO-12.5 group, and ISO-15 group. In the brain tissue of the hippocampus, the expression level of synapsin I and the phosphorylation level of synapsin I S9 and S605 were decreased in both the ISO-12.5 group and ISO-15 group, compared with the CON group, and the expression level of CHIP had a dramatic decline in the ISO-15 group (n = 6, *p < 0.05).

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Phospho-Synapsin I (Ser9) Antibody - #AF3201