Phospho-TFAP2C (Ser434) Antibody | Affinity Biosciences

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Phospho-TFAP2C (Ser434) Antibody - peptide-ELISA analysis of AF7082.

Product:	Phospho-TFAP2C (Ser434) Antibody
Catalog:	AF7082
Description:	Rabbit polyclonal antibody to Phospho-TFAP2C (Ser434)
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	Q92754
RRID:	AB_2843522

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$350	In stock
200ul	$450	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(90%)

Clonality:

Polyclonal

Specificity:

Phospho-TFAP2C (Ser434) Antibody detects endogenous levels of TFAP2C only when phosphorylated at Ser434.

RRID:

AB_2843522
Cite Format: Affinity Biosciences Cat# AF7082, RRID:AB_2843522.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activating enhancer binding protein 2 gamma; Activating enhancer-binding protein 2 gamma; AP2-gamma; AP2C_HUMAN; AP2g; AP2gamma; ERF 1; ERF1; Estrogen receptor factor 1; hAP 2g; hAP2g; TFAP 2C; TFAP 2G; Tfap2c; TFAP2G; Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma); Transcription factor AP 2 gamma; Transcription factor AP-2 gamma; Transcription factor ERF 1; Transcription factor ERF-1; Transcription factor ERF1;

Immunogens

Immunogen:

A synthesized peptide derived from human TFAP2C around the phosphorylation site of Ser434.

Uniprot:

Q92754(AP2C_HUMAN) >>Visit HPA database.

Gene(ID):

TFAP2C(7022)

Sequence:

Q92754 AP2C_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLWKITDNVKYEEDCEDRHDGSSNGNPRVPHLSSAGQHLYSPAPPLSHTGVAEYQPPPYFPPPYQQLAYSQSADPYSHLGEAYAAAINPLHQPAPTGSQQQAWPGRQSQEGAGLPSHHGRPAGLLPHLSGLEAGAVSARRDAYRRSDLLLPHAHALDAAGLAENLGLHDMPHQMDEVQNVDDQHLLLHDQTVIRKGPISMTKNPLNLPCQKELVGAVMNPTEVFCSVPGRLSLLSSTSKYKVTVAEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLREKLDKIGLNLPAGRRKAAHVTLLTSLVEGEAVHLARDFAYVCEAEFPSKPVAEYLTRPHLGGRNEMAARKNMLLAAQQLCKEFTELLSQDRTPHGTSRLAPVLETNIQNCLSHFSLITHGFGSQAICAAVSALQNYIKEALIVIDKSYMNPGDQSPADSNKTLEKMEKHRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Xenopus

Pig

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q92754_AP2C_HUMAN

Function:

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

PTMs:

Sumoylated on Lys-10; which inhibits transcriptional activity.

Subcellular Location:

Nucleus.

Family&Domains:

The PPxY motif mediates interaction with WWOX.

Belongs to the AP-2 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Phospho-TFAP2C (Ser434) Antibody - #AF7082