Product: Phospho-OSR1 (Ser339) Antibody
Catalog: AF7168
Description: Rabbit polyclonal antibody to Phospho-OSR1 (Ser339)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 58kDa; 58kD(Calculated).
Uniprot: O95747
RRID: AB_2843608

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 100ul $350 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Phospho-OSR1 (Ser339) Antibody detects endogenous levels of OSR1 only when phosphorylated at Ser339.
RRID:
AB_2843608
Cite Format: Affinity Biosciences Cat# AF7168, RRID:AB_2843608.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2210022N24Rik; 2810422B09Rik; AI462649; AW209236; KIAA1101; mKIAA1101; OSR 1; OSR1; OTTHUMP00000209062; Oxidative stress responsive 1; Oxidative stress responsive 1 protein; Oxidative stress-responsive 1 protein; OXSR 1; OXSR1; OXSR1_HUMAN; Serine/threonine protein kinase OSR1; Serine/threonine-protein kinase OSR1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O95747 OXSR1_HUMAN:

Ubiquitously expressed in all tissue examined.

Sequence:
MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDESTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKTLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESISNSELFPTTDPVGTLLQVPEQISAHLPQPAGQIATQPTQVSLPPTAEPAKTAQALSSGSGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95747 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K42 Acetylation
K46 Acetylation
K52 Ubiquitination
K105 Ubiquitination
K110 Ubiquitination
K114 Ubiquitination
K139 Ubiquitination
K184 Ubiquitination
T185 Phosphorylation Q9H4A3 (WNK1)
T189 Phosphorylation
K254 Ubiquitination
S263 Phosphorylation
S269 Phosphorylation
K278 Ubiquitination
R287 Methylation
K297 Methylation
K303 Ubiquitination
T310 Phosphorylation
S312 Phosphorylation
S324 Phosphorylation
S325 Phosphorylation Q9H4A3 (WNK1)
T331 Phosphorylation
S339 Phosphorylation P42345 (MTOR)
S347 Phosphorylation
S355 Phosphorylation
S359 Phosphorylation
S369 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
T430 Phosphorylation
S435 Phosphorylation
S443 Phosphorylation
K444 Methylation
R451 Methylation
S510 Phosphorylation

PTMs - O95747 As Enzyme

Substrate Site Source
P55011 (SLC12A2) T203 Uniprot
P55011 (SLC12A2) T207 Uniprot
P55011 (SLC12A2) T212 Uniprot
P55011 (SLC12A2) T217 Uniprot
P55011 (SLC12A2) T230 Uniprot
P55017 (SLC12A3) T46 Uniprot
P55017 (SLC12A3) T55 Uniprot
P55017 (SLC12A3) T60 Uniprot
P55017 (SLC12A3) S73 Uniprot
Q13153 (PAK1) T84 Uniprot
Q13621 (SLC12A1) T95 Uniprot
Q13621 (SLC12A1) T100 Uniprot
Q13621 (SLC12A1) T105 Uniprot
Q13621 (SLC12A1) T118 Uniprot
Q13621 (SLC12A1) S120 Uniprot

Research Backgrounds

Function:

Phosphorylates RELL1, RELL2 and RELT. Phosphorylates PAK1. Phosphorylates PLSCR1 in the presence of RELT.

PTMs:

Autophosphorylated.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed in all tissue examined.

Subunit Structure:

Interacts with PAK1. Interacts with chloride channel proteins SLC12A6 isoform 2, SLC12A1 and SLC12A2 but not with SLC12A4 and SLC12A7, possibly establishing sensor/signaling modules that initiate the cellular response to environmental stress (By similarity). Interacts with RELL1, RELL2 and RELT. Interacts with PLSCR1 in the presence of RELT.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Restrictive clause

 

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