Phospho-MLKL (Ser358) Antibody - #AF7420

Fig. 1: CD47 is overexpressed on necroptotic hepatocytes in the damaged liver of ALF models. A Biochemical evaluation (AST and ALT levels) of the ALF model after APAP induction (left). Representative images of H&E and CD47 staining of liver samples from an ALF model induced by APAP (right) (n = 4). B CD47 expression levels of cell populations within liver tissue (n = 4). C CD47 expression in hepatocytes from normal and APAP-ALF livers (n = 3). D Graphical representation of early apoptotic (Annexin V+/7-AAD−), late apoptotic/necroptotic (Annexin V+/7-AAD+), and necrotic (Annexin V−/7-AAD+) cell populations in liver hepatocytes from normal (n = 4) and APAP-ALF (n = 3) mice. E Expression of RIP3 and pMLKL in liver samples from normal and APAP-ALF groups. F Representative confocal images of liver sections from normal and APAP-ALF mice. Scale bar, 50 μm. G Scatter plot visualization of the Spearman correlation (R) between 121 necroptosis gene scoring and RNA levels of CD47 across samples. H STopover analysis to map the spatial overlap and interactions between cell types in liver tissue from normal and APAP-ALF mice. Highlights include 121 necroptosis gene scores (yellow) and macrophage RNA levels (blue), with overlapping areas shown in green. The plots below represent the three regions. I Violin plot visualizing SIRPα expression levels in different regions based on spatial correlation results. The numbers marked on the plot represent the median values of the SIRPα expression. Bar graph data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t test (A), two-way ANOVA with Sidak’s post hoc test (B, D), and two-tailed test (G). Hepa hepatocytes, Immune immune cells, Endo endothelial cells. Source data are provided as a Source Data file.