TRIM32 Antibody - #AF7574

Product: TRIM32 Antibody
Catalog: AF7574
Description: Rabbit polyclonal antibody to TRIM32
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 72kDa; 72kD(Calculated).
Uniprot: Q13049
RRID: AB_2843938

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TRIM32 Antibody detects endogenous levels of total TRIM32.
RRID:
AB_2843938
Cite Format: Affinity Biosciences Cat# AF7574, RRID:AB_2843938.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

72 kda Tat interacting Protein; 72 kDa Tat-interacting protein; BBS11; E3 ubiquitin-protein ligase TRIM32; HT2A; LGMD2H; Limb girdle muscular dystrophy 2H (autosomal recessive); Limb girdle muscular dystrophy 2H; Muscular dystrophy Hutterite type; TAT interactive protein 72KD; TATIP; TRI32_HUMAN; Trim32; Tripartite Motif Containing Protein 32; Tripartite motif-containing protein 32; Zinc finger protein HT2A;

Immunogens

Immunogen:
Uniprot:

Q13049(TRI32_HUMAN) >>Visit HPA database.

Gene(ID):
TRIM32(22954)
Expression:
Q13049 TRI32_HUMAN:

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

Sequence:
MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGLVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLTRLRELMGELQRRKAALEGVSKDLQARYKAVLQEYGHEERRVQDELARSRKFFTGSLAEVEKSNSQVVEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAVKKPRTVNVEDSWAMEATASAASTSVTFREMDMSPEEVVASPRASPAKQRGPEAASNIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCQGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGSGVVKYSCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQLLVLDCWDHCIKIYSYHLRRYSTP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13049 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
K50 Ubiquitination
K66 Ubiquitination
S72 Phosphorylation
K139 Ubiquitination
K152 Ubiquitination
K167 Ubiquitination
K175 Ubiquitination
K182 Ubiquitination
K204 Ubiquitination
K215 Ubiquitination
Y241 Phosphorylation
K245 Ubiquitination
K247 Ubiquitination
T274 Phosphorylation
K282 Ubiquitination
S306 Phosphorylation
T321 Phosphorylation
S328 Phosphorylation
S335 Phosphorylation
S339 Phosphorylation
K342 Ubiquitination
K359 Ubiquitination
K401 Ubiquitination
K405 Ubiquitination
K471 Ubiquitination
K506 Ubiquitination
S570 Phosphorylation
K604 Ubiquitination
T617 Phosphorylation
K627 Ubiquitination
S651 Phosphorylation

Research Backgrounds

Function:

Has an E3 ubiquitin ligase activity. Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation. May ubiquitinate BBS2. May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo. Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo.

PTMs:

Ubiquitinated.

Subcellular Location:

Cytoplasm.
Note: Localized in cytoplasmic bodies, often located around the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Spleen, thymus, prostate, testis, ovary, intestine, colon and skeletal muscle.

Subunit Structure:

Interacts with DTNBP1. It self-associates.

Family&Domains:

Belongs to the TRIM/RBCC family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

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