Product: Phospho-CDC25C (Ser216) Antibody
Catalog: AF3258
Description: Rabbit polyclonal antibody to Phospho-CDC25C (Ser216)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Horse, Dog, Xenopus
Mol.Wt.: 60kDa; 53kD(Calculated).
Uniprot: P30307
RRID: AB_2834684

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(90%), Dog(90%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Phospho-CDC25C (Ser216) Antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216.
RRID:
AB_2834684
Cite Format: Affinity Biosciences Cat# AF3258, RRID:AB_2834684.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDC 25; Cdc 25C; CDC25; CDC25C; Cell division cycle 25 homolog C; Cell division cycle 25C; Cell division cycle 25C protein; Dual specificity phosphatase Cdc25C; M phase inducer phosphatase 3; M-phase inducer phosphatase 3; Mitosis inducer CDC25; MPIP3; MPIP3_HUMAN; Phosphotyrosine phosphatase; PPP1R60; protein phosphatase 1, regulatory subunit 60;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals.
Sequence:
MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKRCLDLSNLSSGEITATQLTTSADLDETGHLDSSGLQEVHLAGMNHDQHLMKCSPAQLLCSTPNGLDRGHRKRDAMCSSSANKENDNGNLVDSEMKYLGSPITTVPKLDKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSGLYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQGKLRKGLCLKKTVSLCDITITQMLEEDSNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETVAALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYSQEELFNFFLKKPIVPLDTQKRIIIVFHCEFSSERGPRMCRCLREEDRSLNQYPALYYPELYILKGGYRDFFPEYMELCEPQSYCPMHHQDHKTELLRCRSQSKVQEGERQLREQIALLVKDMSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
90
Dog
90
Xenopus
90
Pig
60
Bovine
60
Sheep
60
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30307 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S14 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
S20 Phosphorylation
K27 Ubiquitination
T37 Phosphorylation
S38 Phosphorylation
T48 Phosphorylation P06493 (CDK1)
K52 Ubiquitination
S57 Phosphorylation
S61 Phosphorylation
S64 Phosphorylation
T67 Phosphorylation P06493 (CDK1)
K69 Ubiquitination
S75 Phosphorylation
S122 Phosphorylation P06493 (CDK1)
S129 Phosphorylation
T130 Phosphorylation P06493 (CDK1)
S146 Phosphorylation
S148 Phosphorylation
K151 Ubiquitination
Y165 Phosphorylation
S168 Phosphorylation P45984 (MAPK9) , P53779 (MAPK10) , P45983 (MAPK8) , P06493 (CDK1)
T171 Phosphorylation
T172 Phosphorylation
S191 Phosphorylation Q9H4B4 (PLK3)
S198 Phosphorylation Q9H4B4 (PLK3) , PR:P53350 (hPLK1) , P53350 (PLK1)
S207 Phosphorylation P53778 (MAPK12)
S209 Phosphorylation
Y212 Phosphorylation
S214 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S216 Phosphorylation P27361 (MAPK3) , Q13237 (PRKG2) , Q13535 (ATR) , Q683Z8 (CHK2) , P28482 (MAPK1) , Q16539 (MAPK14) , P49137 (MAPKAPK2) , Q16512 (PKN1) , O14757 (CHEK1) , Q9H4B4 (PLK3) , P27448 (MARK3) , P43405 (SYK) , Q8TDC3 (BRSK1) , O96017 (CHEK2)
K233 Ubiquitination
T236 Phosphorylation P68400 (CSNK2A1)
K240 Ubiquitination
K244 Ubiquitination
S247 Phosphorylation Q15418 (RPS6KA1)
K251 Ubiquitination
K260 Ubiquitination
S263 Phosphorylation
S287 Phosphorylation
K288 Ubiquitination
K298 Ubiquitination
K303 Ubiquitination
K317 Ubiquitination
K360 Ubiquitination
Y401 Phosphorylation
K413 Ubiquitination
Y416 Phosphorylation
S449 Phosphorylation
K452 Ubiquitination
K469 Ubiquitination
S472 Phosphorylation

Research Backgrounds

Function:

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

PTMs:

Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216. However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated on Ser-129 and/or Thr-130, interacts with PLK1.

(Microbial infection) Interacts with HIV-1 Vpr; this interaction inactivates CDC25C phosphatase activity.

Family&Domains:

Belongs to the MPI phosphatase family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

References

1). Yuexian Li et al. ITGB1 enhances the Radioresistance of human Non-small Cell Lung Cancer Cells by modulating the DNA damage response and YAP1-induced Epithelial-mesenchymal Transition. International Journal of Biological Sciences 2021 Jan 18;17(2):635-650. (PubMed: 33613118) [IF=9.2]

Application: WB    Species: Human    Sample: NSCLC cell

Figure 7 ITGB1 expression correlates with the expression of proteins related to the ATM/CHK2/CDC25c pathway. A. The relationship between ITGB1 expression and that of 34 genes in the DNA-DSB response pathways. B-D. Protein levels of ATM (p-ATM), CHK2 (p-CHK2), and CDC25c (p-CDC25c) were detected by western blotting in shITGB1 and ITGB1 overexpression groups.

Application: WB    Species: human    Sample: NSCLC cells

Figure 7.| ITGB1 expression correlates with the expression of proteins related to the ATM/CHK2/CDC25c pathway. A. The relationship between ITGB1 expression and that of 34 genes in the DNA-DSB response pathways. B-D. Protein levels of ATM (p-ATM), CHK2 (p-CHK2), and CDC25c (p-CDC25c) were detected by western blotting in shITGB1 and ITGB1 overexpression groups.

2). Li M et al. ATM deficiency aggravates the progression of liver fibrosis induced by carbon tetrachloride in mice. Toxicology 2022 Dec 13; (PubMed: 36526012) [IF=4.5]

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