Phospho-VE-Cadherin (Tyr731) Antibody - #AF3265
|Product:||Phospho-VE-Cadherin (Tyr731) Antibody|
|Description:||Rabbit polyclonal antibody to Phospho-VE-Cadherin (Tyr731)|
|Application:||WB IHC IF/ICC|
|Reactivity:||Human, Mouse, Rat|
|Prediction:||Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken|
*The optimal dilutions should be determined by the end user.
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3265, RRID:AB_2834691.
7B 4; 7B4; 7B4 antigen; CADH5_HUMAN; Cadherin 5; Cadherin 5 type 2; Cadherin 5, type 2 (vascular endothelium); Cadherin 5, type 2, VE cadherin (vascular epithelium); cadherin, vascular endothelial; cadherin, vascular endothelial, 1; Cadherin-5; Cadherin5; CD 144; CD144; CD144 antigen; CDH 5; CDH5; CDH5 protein; Endothelial specific cadherin; FLJ17376; OTTHUMP00000174777; Vascular endothelial cadherin; Vascular epithelium cadherin; VE Cad; VE-cadherin; VEC;
Endothelial tissues and brain.
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P33151 As Substrate
Cadherins are calcium-dependent cell adhesion proteins (By similarity). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions (By similarity). It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 and MPP5 to establish and maintain correct endothelial cell polarity and vascular lumen (By similarity). These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB (By similarity).
Cell junction. Cell membrane>Single-pass type I membrane protein.
Note: Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions.
Endothelial tissues and brain.
Interacts (via cadherin 5 domain) with PTPRB (By similarity). Interacts with TRPC4. Interacts with KRIT1. Interacts with PARD3 (By similarity). Interacts with RTN4 (isoform B). Interacts with MPP5; the interaction promotes MPP5 localization to cell junctions and is required for CDH5-mediated vascular lumen formation and endothelial cell.
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Application: WB Species: Human Sample: HMVEC cells
Application: WB Species: Human Sample: HUVECs
Application: IHC Species: Mouse Sample: aortic endothelial cells
Application: WB Species: Mouse Sample: aortic endothelial cells
Application: IF/ICC Species: Mouse Sample: aortic endothelial cells
Application: WB Species: human Sample: HUVECs
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